Stimulated Raman scattering (SRS) microscopy has the capability to simultaneously visualize the spatial distribution of different biomolecules, but it remains challenging to reach super-resolution. To achieve this goal, a deconvolution algorithm, A-PoD, was developed and combined with SRS microscopy, enabling examination of nanoscopic biomolecular distribution and subcellular metabolic activity in cells and tissues.
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References
Freudiger, C. W. et al. Label-free biomedical imaging with high sensitivity by stimulated Raman scattering microscopy. Science 322, 1857–1861 (2008). This article reported SRS microscopy.
Shi, L. et al. Optical imaging of metabolic dynamics in animals. Nat. Commun. 9, 2995 (2018). The article introduces DO-SRS microscopy.
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This is a summary of: Jang, H. et al. Super-resolution SRS microscopy with A-PoD. Nat. Methods https://doi.org/10.1038/s41592-023-01779-1 (2023).
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A deconvolution algorithm to achieve super-resolution stimulated Raman scattering imaging. Nat Methods 20, 361–362 (2023). https://doi.org/10.1038/s41592-023-01780-8
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DOI: https://doi.org/10.1038/s41592-023-01780-8
