The ability to measure protein complexes in single cells is currently limited to a very small number of targets. Combining a proximity ligation assay with single-cell sequencing creates the ability to measure hundreds of extracellular protein complexes and thousands of mRNAs in individual cells.
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References
Stoeckius, M. et al. Simultaneous epitope and transcriptome measurement in single cells. Nat. Methods 14, 865–868 (2017). This paper, alongside ref. 2, was among the first to show protein measurements combined with single-cell sequencing.
Peterson, V. M. et al. Multiplexed quantification of proteins and transcripts in single cells. Nat. Biotechnol. 35, 936–939 (2017). This paper, alongside ref. 1, was among the first to show protein measurements combined with single-cell sequencing.
Budnik, B. et al. SCoPE-MS: mass spectrometry of single mammalian cells quantifies proteome heterogeneity during cell differentiation. Genome Biol. 19, Article number: 161 (2018). This paper showed quantification of hundreds of proteins in a single cell by mass spectrometry.
Fredriksson, S. et al. Protein detection using proximity-dependent DNA ligation assays. Nat. Biotechnol. 20, 473–477 (2002). This paper presents the foundational assay for Prox-seq: the proximity ligation assay.
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This is a summary of: Vistain, L. et al. Quantification of extracellular proteins, protein complexes and mRNAs in single cells by proximity sequencing. Nat. Methods https://doi.org/10.1038/s41592-022-01684-z (2022)
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Proximity sequencing for single-cell measurement of protein complexes. Nat Methods 19, 1528–1529 (2022). https://doi.org/10.1038/s41592-022-01686-x
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DOI: https://doi.org/10.1038/s41592-022-01686-x
