Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Proximity sequencing for single-cell measurement of protein complexes

The ability to measure protein complexes in single cells is currently limited to a very small number of targets. Combining a proximity ligation assay with single-cell sequencing creates the ability to measure hundreds of extracellular protein complexes and thousands of mRNAs in individual cells.

This is a preview of subscription content, access via your institution

Access options

Buy article

Get time limited or full article access on ReadCube.

$32.00

All prices are NET prices.

Fig. 1: Proximity sequencing.

References

  1. Stoeckius, M. et al. Simultaneous epitope and transcriptome measurement in single cells. Nat. Methods 14, 865–868 (2017). This paper, alongside ref. 2, was among the first to show protein measurements combined with single-cell sequencing.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Peterson, V. M. et al. Multiplexed quantification of proteins and transcripts in single cells. Nat. Biotechnol. 35, 936–939 (2017). This paper, alongside ref. 1, was among the first to show protein measurements combined with single-cell sequencing.

    Article  CAS  PubMed  Google Scholar 

  3. Budnik, B. et al. SCoPE-MS: mass spectrometry of single mammalian cells quantifies proteome heterogeneity during cell differentiation. Genome Biol. 19, Article number: 161 (2018). This paper showed quantification of hundreds of proteins in a single cell by mass spectrometry.

    Article  PubMed  PubMed Central  Google Scholar 

  4. Fredriksson, S. et al. Protein detection using proximity-dependent DNA ligation assays. Nat. Biotechnol. 20, 473–477 (2002). This paper presents the foundational assay for Prox-seq: the proximity ligation assay.

    Article  CAS  PubMed  Google Scholar 

Download references

Additional information

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

This is a summary of: Vistain, L. et al. Quantification of extracellular proteins, protein complexes and mRNAs in single cells by proximity sequencing. Nat. Methods https://doi.org/10.1038/s41592-022-01684-z (2022)

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Proximity sequencing for single-cell measurement of protein complexes. Nat Methods 19, 1528–1529 (2022). https://doi.org/10.1038/s41592-022-01686-x

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41592-022-01686-x

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing