A combination of light-sheet fluorescence microscopy (LSFM) with structured illumination doubles resolving power over LSFM alone. We show a practical implementation using a single objective for illumination and fluorescence detection and demonstrate its use for rapid volumetric imaging.
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References
Stelzer, E. H. K. et al. Light sheet fluorescence microscopy. Nat. Rev. Methods Primers 1, 73 (2021). A primer of the current state of the art in light-sheet fluorescence microscopy (LSFM).
Gustafsson, M. G. L. Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy. J. Microsc. 198, 82–87 (2000). Seminal paper for structured illumination microscopy, which was used here to improve the resolution in LSFM.
Dunsby, C. Optically sectioned imaging by oblique plane microscopy. Opt. Express 16, 20306–20316 (2008). Seminal paper for oblique plane microscopy, which was used here as a starting point for combining LSFM with structured illumination.
Chang, B.-J. et al. Real-time multi-angle projection imaging of biological dynamics. Nat. Methods 18, 829–834 (2021). A study describing a method to form projections from a 3D volume, which conceptually can be applied to the work presented here.
Chang, B.-J., Meza, V. D. P. & Stelzer, E. H. K. csiLSFM combines light-sheet fluorescence microscopy and coherent structured illumination for a lateral resolution below 100 nm. Proc. Natl Acad. Sci. USA 114, 4869–4874 (2017). A previous effort to combine structured illumination with LSFM using two illumination and one detection objective.
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This is a summary of: Chen, B. et al. Resolution doubling in light-sheet microscopy via oblique plane structured illumination. Nat. Methods https://doi.org/10.1038/s41592-022-01635-8 (2022).
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Doubling the resolution of light-sheet fluorescence microscopy. Nat Methods 19, 1355–1356 (2022). https://doi.org/10.1038/s41592-022-01636-7
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DOI: https://doi.org/10.1038/s41592-022-01636-7
