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An in situ method for tagging chromatin-associated RNAs

RNA comprises a substantial fraction of eukaryotic chromatin, but techniques to identify and map RNAs are cumbersome. We adapted existing tagmentation-based profiling techniques to enable chromatin-associated RNAs to be profiled in a simple workflow, enhancing the capability to identify regulatory RNAs.

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Fig. 1: RT&Tag procedure.

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This is a summary of: Khyzha, N., Henikoff, S. & Ahmad, K. Profiling RNA at chromatin targets in situ by antibody-targeted tagmentation. Nat. Methods https://doi.org/10.1038/s41592-022-01618-9 (2022)

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An in situ method for tagging chromatin-associated RNAs. Nat Methods 19, 1351–1352 (2022). https://doi.org/10.1038/s41592-022-01619-8

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  • DOI: https://doi.org/10.1038/s41592-022-01619-8

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