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BATTLES: high-throughput screening of antigen recognition under force

In vivo, forces applied to molecular interactions between T cells and antigen-presenting cells are essential for specific foreign antigen recognition. A new technology, BATTLES, applies force to thousands of T cells interacting with tens of candidate antigens to identify antigens capable of efficient T cell activation. The method improves throughput over current methods that profile force-dependent interactions.

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Fig. 1: Applying BATTLEs to map the sequence specificity of T cell activation under load.

References

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This is a summary of: Feng, Y., Zhao, X., White, A. K., Garcia, K. C. & Fordyce, P. M. A bead-based method for high-throughput mapping of the sequence- and force-dependence of T cell activation. Nat. Methods https://doi.org/10.1038/s41592-022-01592-2 (2022).

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BATTLES: high-throughput screening of antigen recognition under force. Nat Methods (2022). https://doi.org/10.1038/s41592-022-01593-1

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  • DOI: https://doi.org/10.1038/s41592-022-01593-1

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