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PROTEOMICS

Towards resolving proteomes in single cells

Nature Methods volume 18, page 856 (2021)Cite this article

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A Publisher Correction to this article was published on 16 September 2021

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Development of methods for single-cell proteomics increases depth and throughput

Single-cell RNA sequencing approaches provide an indirect readout for protein levels in a given cell, but these measurements tend to be sparse for many transcripts. Additionally, protein stability influences the proteome, and this information cannot be derived from RNA-seq, making it necessary to directly quantify the proteome in single cells. Antibody-based methods have been employed to quantify proteomes in single cells, but these have limited specificity. Recently, liquid chromatography–mass spectrometry (LC–MS)-based proteomics approaches have made headway in increasing the throughput and depth of single-cell measurements.

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Research papers

  • Specht, H. et al. Single-cell proteomic and transcriptomic analysis of macrophage heterogeneity using SCoPE2. Genome Biol. 22, 50 (2021).

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  • Schoof, E. M. et al. Quantitative single-cell proteomics as a tool to characterize cellular hierarchies. Nat. Commun. 12, 3341 (2021).

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  1. Nature Methods http://www.nature.com/nmeth

    Arunima Singh

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  1. Arunima Singh
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Correspondence to Arunima Singh.

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Singh, A. Towards resolving proteomes in single cells. Nat Methods 18, 856 (2021). https://doi.org/10.1038/s41592-021-01243-y

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  • DOI: https://doi.org/10.1038/s41592-021-01243-y

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