Ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC–HRMS) variants currently represent the best tools to tackle the challenges of complexity and lack of comprehensive coverage of the metabolome. UHPLC offers flexible and efficient separation coupled with high-sensitivity detection via HRMS, allowing for the detection and identification of a broad range of metabolites. Here we discuss current common strategies for UHPLC–HRMS-based metabolomics, with a focus on expanding metabolome coverage.
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A.R.F. and S.A. are supported by the European Union’s Horizon 2020 research and innovation program, project PlantaSYST (SGA-CSA 739582 under FPA 664620). L.P.d.S. is supported by the European Union’s Horizon 2020 research and innovation program, project INCREASE (GA 862862).
The authors declare no competing interests.
Peer review information Nature Methods thanks Warwick Dunn, Albert Fornace and the other, anonymous, reviewer(s) for their contribution to the peer review of this work. Allison Doerr was the primary editor on this article and managed its editorial process and peer review in collaboration with the rest of the editorial team.
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Perez de Souza, L., Alseekh, S., Scossa, F. et al. Ultra-high-performance liquid chromatography high-resolution mass spectrometry variants for metabolomics research. Nat Methods (2021). https://doi.org/10.1038/s41592-021-01116-4