Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

SENSORS AND PROBES

Detecting intracellular PPIs with CLEM

Cell Chem. Biol. 26, 1407–1416 (2019).

Visualizing protein–protein interactions (PPIs) with correlative light and electron microscopy (CLEM) has mostly been restricted to extracellular interactions. To overcome this limitation, Boassa et al. have generated a split-miniSOG reporter. miniSOG has the attractive property of being both fluorescent and able to generate reactive oxygen species upon illumination. These species can then be harnessed to precipitate 3,3′-diaminobenzidine, which reacts with osmium tetroxide to form an EM-visible precipitate. To detect PPIs, the researchers split miniSOG and fused the resulting fragments to the proteins of interest. As the two fragments exhibit low affinity for each other, they only reconstitute in the presence of a PPI, and the reconstitution is reversible when the PPI is no longer present. The researchers illustrate the utility of their split-miniSOG by visualizing the interaction between Fos and Jun in cultured cells, as well as other PPIs, using both light and electron microscopy.

Author information

Affiliations

Authors

Corresponding author

Correspondence to Nina Vogt.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Vogt, N. Detecting intracellular PPIs with CLEM. Nat Methods 16, 1205 (2019). https://doi.org/10.1038/s41592-019-0681-7

Download citation

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing