Monitoring molecular jumps

Eilers, Y. et al. Proc. Natl Acad. Sci. USA 115, 6117–6122 (2018).

The recently described MINFLUX nanoscopy method allows for high-resolution monitoring of single molecules with a small fraction of the photons needed for conventional super-resolution microscopy. Although the method was shown to work well for high-resolution imaging of DNA origami structures and for single-particle tracking with high spatiotemporal resolution, its benefits for the investigation of single-particle movements were not fully explored. Eilers et al. now show that MINFLUX can be used to monitor molecular movements of a few nanometers at rates that far surpass the spatiotemporal resolution of conventional approaches. They demonstrated their approach by studying the thermal fluctuation of fluorophore-labeled DNA strands, and achieved ~2-nanometer localization precision with measurements as short as 400 microseconds. These results highlight the power of this approach for studying the dynamics of single molecules.

Author information



Corresponding author

Correspondence to Rita Strack.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Strack, R. Monitoring molecular jumps. Nat Methods 15, 572 (2018). https://doi.org/10.1038/s41592-018-0091-2

Download citation


Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing