Chemical Biology

Controlling protein function with HCV protease

Two methods for controlling protein activities with small molecules provide a general solution to a long-standing challenge in mammalian synthetic biology.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

Fig. 1: In LInC and StaPLs, a linker using HCV protease and its cognate recognition sequence is installed between a protein of interest and a regulatory domain.

Marina Corral Spence/Springer Nature

References

  1. 1.

    Tague, E. P., Dotson, H. L., Tunney, S. N., Sloas, D. C. & Ngo, J. T. Nat. Methods https://doi.org/10.1038/s41592-018-0042-y (2018).

  2. 2.

    Jacobs, C. L., Badiee, R. K. & Lin, M. Z. Nat. Methods https://doi.org/10.1038/s41592-018-0041-z (2018).

  3. 3.

    Ruder, W. C., Lu, T. & Collins, J. J. Science 333, 1248–1252 (2011).

    Article  PubMed  CAS  Google Scholar 

  4. 4.

    Lienert, F., Lohmueller, J. J., Garg, A. & Silver, P. A. Nat. Rev. Mol. Cell Biol. 15, 95–107 (2014).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  5. 5.

    Spencer, D. M., Wandless, T. J., Schreiber, S. L. & Crabtree, G. R. Science 262, 1019–1024 (1993).

    Article  PubMed  CAS  Google Scholar 

  6. 6.

    Rakhit, R., Navarro, R. & Wandless, T. J. Chem. Biol. 21, 1238–1252 (2014).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  7. 7.

    McCauley, J. A. & Rudd, M. T. Curr. Opin. Pharmacol. 30, 84–92 (2016).

    Article  PubMed  CAS  Google Scholar 

  8. 8.

    Lin, M. Z., Glenn, J. S. & Tsien, R. Y. Proc. Natl Acad. Sci. USA 105, 7744–7749 (2008).

    Article  PubMed  Google Scholar 

  9. 9.

    Chung, H. K. et al. Nat. Chem. Biol. 11, 713–720 (2015).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  10. 10.

    Pu, J., Chronis, I., Ahn, D. & Dickinson, B. C. J. Am. Chem. Soc. 137, 15996–15999 (2015).

    Article  PubMed  PubMed Central  CAS  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to Bryan C. Dickinson.

Ethics declarations

Competing interests

The author declares no competing interests.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Dickinson, B.C. Controlling protein function with HCV protease. Nat Methods 15, 489–490 (2018). https://doi.org/10.1038/s41592-018-0043-x

Download citation

Further reading

Search

Sign up for the Nature Briefing newsletter for a daily update on COVID-19 science.
Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing