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DNA modification

Direct methylation sequencing

Nature Chemical Biology volume 19, pages 932–933 (2023)Cite this article

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Sodium bisulfite is widely used in methylation sequencing, yet it degrades DNA, and on its own, it does not discriminate methylated cytosine from its oxidized derivative, 5-hydroxymethylcytosine. A new bisulfite-free technique uses enzymatic modification of DNA for direct and accurate methylation mapping.

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Fig. 1: Direct methylation sequencing by enzymatic modification of DNA.

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Authors and Affiliations

  1. Altos Labs Cambridge Institute of Science, Granta Park, Cambridge, UK

    Abdulkadir Abakir & Wolf Reik

  2. Epigenetics Programme, Babraham Institute, Cambridge, UK

    Wolf Reik

  3. Wellcome Sanger Institute, Wellcome Genome Campus, Cambridge, UK

    Wolf Reik

  4. Wellcome Trust, Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge, UK

    Wolf Reik

  5. Centre for Trophoblast Research, University of Cambridge, Cambridge, UK

    Wolf Reik

Authors
  1. Abdulkadir Abakir
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  2. Wolf Reik
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Correspondence to Abdulkadir Abakir.

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Competing interests

A.A. and W.R. are employed by Altos Labs. W.R. is a consultant and shareholder of CEGX.

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Abakir, A., Reik, W. Direct methylation sequencing. Nat Chem Biol 19, 932–933 (2023). https://doi.org/10.1038/s41589-023-01356-9

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