Genome-wide CRISPR screening coupled with ATAC-see uncovered modulators that influence global chromatin accessibility. Notably, TFDP1 emerged as a pivotal modulator of chromatin accessibility that acts by controlling histone transcription. Depletion of TFDP1 induced a global elevation in accessibility, enhancing the efficiency of genome editing and iPS cell reprogramming.
This is a preview of subscription content, access via your institution
Access options
Access Nature and 54 other Nature Portfolio journals
Get Nature+, our best-value online-access subscription
$29.99 / 30 days
cancel any time
Subscribe to this journal
Receive 12 print issues and online access
$209.00 per year
only $17.42 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Klemm, S. L., Shipony, Z. & Greenleaf, W. J. Chromatin accessibility and the regulatory epigenome. Nat. Rev. Genet. 20, 207–220 (2019). A review article that describes how chromatin accessibility is measured and regulated.
Chen, X. et al. ATAC-see reveals the accessible genome by transposase-mediated imaging and sequencing. Nat. Methods 13, 1013–1020 (2016). This paper presents the ATAC-see method.
Replogle, J. M. et al. Mapping information-rich genotype-phenotype landscapes with genome-scale Perturb-seq. Cell 185, 2559–2575.e28 (2022). This paper presents the genome-wide single-cell CRISPR screen.
Pierce, S. E., Granja, J. M. & Greenleaf, W. J. High-throughput single-cell chromatin accessibility CRISPR screens enable unbiased identification of regulatory networks in cancer. Nat. Commun. 12, 2969 (2021). This paper presents simultaneous detection of chromatin accessibility and integrated sgRNA sequences at the single-cell level.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This is a summary of: Ishii, S. et al. Genome-wide ATAC-see screening identifies TFDP1 as a modulator of global chromatin accessibility. Nat. Genet. https://doi.org/10.1038/s41588-024-01658-1 (2024).
Rights and permissions
About this article
Cite this article
Proteins shaping global chromatin accessibility. Nat Genet 56, 367–368 (2024). https://doi.org/10.1038/s41588-024-01667-0
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41588-024-01667-0
