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BRD4 interacts with NIPBL and BRD4 is mutated in a Cornelia de Lange–like syndrome

A Publisher Correction to this article was published on 12 February 2018

This article has been updated


We found that the clinical phenotype associated with BRD4 haploinsufficiency overlapped with that of Cornelia de Lange syndrome (CdLS), which is most often caused by mutation of NIPBL. More typical CdLS was observed with a de novo BRD4 missense variant, which retained the ability to coimmunoprecipitate with NIPBL, but bound poorly to acetylated histones. BRD4 and NIPBL displayed correlated binding at super-enhancers and appeared to co-regulate developmental gene expression.

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Fig. 1: BRD4 mutations in CdLS and CdLS-like disorders.
Fig. 2: Binding of wild-type BRD4 and the BRD4 Tyr430Cys variant to histone and non-histone proteins.

Change history

  • 03 June 2019

    An amendment to this paper has been published and can be accessed via a link at the top of the paper.


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We thank the CdLS Foundation of the UK and Ireland and particularly the families of the affected children for their time and support for the research. G.O., M.A., H.B., W.A.B., M.M.P. and D.R.F. were funded by the MRC University Unit award to the University of Edinburgh for the MRC Human Genetics Unit. The work of A.v.K. was supported by Carnegie Trust Research Incentive Grant 70382. The DDD study presents independent research commissioned by the Health Innovation Challenge Fund (grant HICF-1009-003), a parallel funding partnership between the Wellcome Trust and the Department of Health, and the Wellcome Trust Sanger Institute (grant WT098051). The views expressed in this publication are those of the authors and not necessarily those of the Wellcome Trust or the Department of Health. The research team acknowledges the support of the National Institute for Health Research through the Comprehensive Clinical Research Network.

Author information





W.A.B., M.M.P. and D.R.F. conceived the study. D.R.F., W.A.B. and M.M.P. wrote the manuscript. All of the authors read and commented on the manuscript. G.O., M.A., H.B., N.C., M.M.P. and the DDD study generated the molecular biology and animal model data. A.v.K. generated and analyzed the mass spectrometry data. F.J.S., E.W., A.R. and S.M.P. provided expert clinical interpretation and details of the phenotype for each affected individual. A.B. performed the meta-analysis of the reported deletion cases. J.R. provided expert technical advice and cell reagents. G.R.G. performed the genomic and transcriptomic informatic analysis.

Corresponding authors

Correspondence to Wendy A. Bickmore or Madapura M. Pradeepa or David R. FitzPatrick.

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The authors declare no competing financial interests.

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Supplementary information

Supplementary Text and Figures

Supplementary Figures 1–20, Supplementary Tables 1–3 and Supplementary Note

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Supplementary Table 4

BRD4 immunoprecipitation data

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Olley, G., Ansari, M., Bengani, H. et al. BRD4 interacts with NIPBL and BRD4 is mutated in a Cornelia de Lange–like syndrome. Nat Genet 50, 329–332 (2018).

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