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Relating DNA base-pairing in aqueous media to DNA polymerase fidelity

Nature Reviews Chemistry volume 1, Article number: 0074 (2017) | Download Citation

Controversy surrounds the perceived absence of a relationship between DNA polymerase fidelity (kinetic discrimination) and free energy changes determined from DNA melting studies (thermodynamic discrimination). Thermodynamic discrimination together with aqueous solvent effects can account for kinetic fidelities on the order of those observed experimentally.

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Acknowledgements

The authors thank C. H. Mak and K. Oertell for insightful discussions, and J. G. Bertram for collating the data. This work was supported by the US National Institutes of Health (Grant Nos ES012259, U19CA177547 and GM214422 to M.F.G.).

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Affiliations

  1. Department of Biological Sciences, University of Southern California.

    • John Petruska
    •  & Myron F. Goodman
  2. Department of Chemistry, University of Southern California, Los Angeles, California 90089, USA.

    • Myron F. Goodman

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Competing interests

The authors declare no competing interests.

Corresponding author

Correspondence to Myron F. Goodman.

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DOI

https://doi.org/10.1038/s41570-017-0074

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