Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Super-resolution microscopy

Tracking nanoscopic motion with minima of light

Two papers in Science demonstrate tracking of the stepping motion of the kinesin motor protein with nanometric spatial precision and sub-millisecond temporal resolution by using MINFLUX, a highly photon-efficient single-molecule localization technique.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: Localizing molecules using a minimum of light.

References

  1. Wolff, J. O. et al. Science 379, 1004–1010 (2023).

    Article  ADS  Google Scholar 

  2. Deguchi, T. et al. Science 379, 1010–1015 (2023).

    Article  ADS  Google Scholar 

  3. Yildiz, A. et al. Science 300, 2061–2065 (2003).

    Article  ADS  Google Scholar 

  4. Yildiz, A., Tomishige, M., Vale, R. D. & Selvin, P. R. Science 303, 676–678 (2004).

    Article  ADS  Google Scholar 

  5. Svoboda, K., Schmidt, C. F., Schnapp, B. J. & Block, S. M. Nature 365, 721–727 (1993).

    Article  ADS  Google Scholar 

  6. Schnitzer, M. J. & Block, S. M. Nature 388, 386–390 (1997).

    Article  ADS  Google Scholar 

  7. Ortega-Arroyo, J. & Kukura, P. Phys. Chem. Chem. Phys. 14, 15625 (2012).

    Article  Google Scholar 

  8. Balzarotti, F. et al. Science 355, 606–612 (2017).

    Article  ADS  Google Scholar 

  9. Masullo, L. A., Lopez, L. F. & Stefani, F. D. Biophys. Rep. 2, 100036 (2022).

    Google Scholar 

  10. Schmidt, R. et al. Nat. Commun. 12, 1478 (2021).

    Article  ADS  Google Scholar 

  11. Masullo, L. A. et al. Nano Lett. 21, 840–846 (2021).

    Article  ADS  Google Scholar 

  12. Masullo, L. A. et al. Light Sci. Appl. 11, 199 (2022).

    Article  ADS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Fernando D. Stefani.

Ethics declarations

Competing interests

The author declares no competing interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Stefani, F.D. Tracking nanoscopic motion with minima of light. Nat. Photon. 17, 552–553 (2023). https://doi.org/10.1038/s41566-023-01239-4

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41566-023-01239-4

This article is cited by

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing