PROTEIN PATTERNING

Moving hydrogels to the fourth dimension

Materials that permit spatiotemporal control of biomolecule presentation have long been a challenge in the field. A method has now been developed to reversibly pattern cell-laden hydrogels with site-specifically immobilized proteins using sortase-mediated transpeptidation without compromising bioactivity.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

Fig. 1: Photopatterning and photorelease of sortase-tag enhanced protein ligation (STEPL)-modified proteins in hydrogels.

References

  1. 1.

    Burdick, J. A. & Murphy, W. L. Nat. Commun. 3, 1269 (2012).

  2. 2.

    Shadish, J. A., Benuska, G. M. & DeForest, C. A. Nat. Mater. https://doi.org/10.1038/s41563-019-0367-7 (2019).

  3. 3.

    Murphy, W. L., McDevitt, T. C. & Engler, A. J. Nat. Mater. 13, 547–557 (2014).

  4. 4.

    Lee, S., Moon, J. J. & West, J. L. Biomaterials 29, 2962–2968 (2008).

  5. 5.

    Yang, C., Tibbitt, M. W., Basta, L. & Anseth, K. S. Nat. Mater. 13, 645–652 (2014).

  6. 6.

    Wylie, R. G. et al. Nat. Mater. 10, 799–806 (2011).

  7. 7.

    DeForest, C. A. & Tirrell, D. A. Nat Mater 14, 523–531 (2015).

  8. 8.

    Mao, H., Hart, S. A., Schink, A. & Pollok, B. A. J. Am. Chem. Soc. 126, 2670–2671 (2004).

  9. 9.

    Caliari, S. R. & Burdick, J. A. Nat. Methods 13, 405–414 (2016).

  10. 10.

    Loebel, C., Mauck, R. L. & Burdick, J. A. Nat. Mater. 18, 883–891 (2019).

Download references

Author information

Correspondence to Jason A. Burdick.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Galarraga, J.H., Burdick, J.A. Moving hydrogels to the fourth dimension. Nat. Mater. 18, 914–915 (2019) doi:10.1038/s41563-019-0458-5

Download citation

Further reading