Abstract
The evolution and great diversity of genome size has been of long-standing interest to biologists, but has seldom been investigated on a broad phylogenetic scale. Here we present a comparative quantitative analysis of factors shaping genome size evolution in amphibians, the extant class of vertebrates with the largest variation in genome size. We find that amphibian genomes have undergone saltations in size, although these are rare and the evolutionary history of genome size in amphibians has otherwise been one of gradual, time-dependent variation (that is, Brownian motion). This macroevolutionary homogeneity is remarkable given the evolutionary and ecological diversity of most other aspects of the natural history of amphibians. Contrary to previous claims, we find no evidence for associations between life cycle complexity and genome size despite the high diversity of reproductive modes and the multiple events of independent evolution of divergent life cycles in the group. Climate (temperature and humidity) affects genome size indirectly, at least in frogs, as a consequence of its effect on premetamorphic developmental period, although directionality of the relationship between developmental period and genome size is not unequivocal.
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Data availability
All genome size measurements generated for this study are available in the supplementary information.
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Acknowledgements
We would like to thank C. Spencer at the Museum of Vertebrate Zoology (Berkeley), M.-O. Rödel and F. Tillack at the Museum für Naturkunde (Berlin), S. Loader and J. Streicher at the Natural History Museum (London), B. Alvarez and I. Martinez-Solano at the Museo Nacional de Ciencias Naturales (Madrid), V. Gvoždík at the Academy of Sciences (Brno) for tissue loans and L. Asencio Vazquez for assistance with laboratory work. M.W. and D.J.G. thank the many persons and organizations that have facilitated the collection of caecilian materials used in this work, especially S. Maddock and the Direction de l’Environment, de l’Aménagement et du Logement and the Conseil Scientifique Régional du Patrimonie Naturel (Guyane). Funding was provided by Spanish National Research Plan (CGL2017-83407-P) awarded to I.G.-M., Swiss National Science Fund (P2BSP3_158846) awarded to H.C.L. and the Darwin Initiative (19-002) awarded to D.J.G.
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H.C.L., I.G.-M., D.J.G. and M.W. were all involved in planning and executing the project. D.J.G. and M.W. contributed key samples and H.C.L. obtained de novo genome size estimates and performed data analyses with input from I.G.-M. H.C.L. wrote the first version of the manuscript, to which all other authors thereafter contributed.
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Liedtke, H.C., Gower, D.J., Wilkinson, M. et al. Macroevolutionary shift in the size of amphibian genomes and the role of life history and climate. Nat Ecol Evol 2, 1792–1799 (2018). https://doi.org/10.1038/s41559-018-0674-4
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DOI: https://doi.org/10.1038/s41559-018-0674-4
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