One of the long-standing mysteries of evolutionary genomics is the source of the wide phylogenetic diversity in genome nucleotide composition (G + C versus A + T), which must be a consequence of interspecific differences in mutation bias, the efficiency of selection for different nucleotides or a combination of the two. We demonstrate that although genomic G + C composition is strongly driven by mutation bias, it is also substantially modified by direct selection and/or as a by-product of biased gene conversion. Moreover, G + C composition at fourfold redundant sites is consistently elevated above the neutral expectation—more so than for any other class of sites.
Subscribe to Journal
Get full journal access for 1 year
only $8.25 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Rent or Buy article
Get time limited or full article access on ReadCube.
All prices are NET prices.
Sueoka, N. Proc. Natl Acad. Sci. USA 48, 582–592 (1962).
Gu, X., Hewett-Emmett, D. & Li, W. H. Genetica 102–103, 383–391 (1998).
Chen, S. L., Lee, W., Hottes, A. K., Shapiro, L. & McAdams, H. H. Proc. Natl Acad. Sci. USA 101, 3480–3485 (2004).
Lynch, M. The Origin of Genome Architecture. Sinauer Associates: Sunderland, 2007.
Rocha, E. P. C. & Feil, E. J. PLoS Genet. 6, e1001104 (2010).
Hershberg, R. & Petrov, D. A. PLoS Genet. 6, e1001115 (2010).
Hildebrand, F., Meyer, A. & Eyre-Walker, A. PLoS Genet. 6, e1001107 (2010).
Lynch, M. Proc. Natl Acad. Sci. USA 107, 961–968 (2010).
Lynch, M. et al. Nat. Rev. Genet. 17, 704–714 (2016).
Li, W. H. J. Mol. Evol. 24, 337–345 (1987).
Bulmer, M. Genetics 129, 897–907 (1991).
McVean, G. A. T. & Charlesworth, B. Genet. Res. 74, 145–158 (1999).
Raghavan, R., Kelkar, Y. D. & Ochman, H. Proc. Natl Acad. Sci. USA 109, 14504–14507 (2012).
Kelkar, Y. D., Phillips, D. S. & Ochman, H. G3 5, 1247–1252 (2015).
Marais, G., Mouchiroud, D. & Duret, L. Genet. Res. 81, 79–87 (2003).
Mancera, E., Bourgon, R., Brozzi, A., Huber, W. & Steinmetz, L. M. Nature 454, 479–485 (2008).
Galtier, N., Duret, L., Glemin, S. & Ranwez, V. Trends Genet. 25, 1–5 (2009).
Pessia, E. et al. Genome Biol. Evol. 4, 675–682 (2012).
Williams, A. L. et al. eLife 4, e04637 (2015).
Mugal, C. F., Weber, C. C. & Ellegren, H. Bioessays 37, 1317–1326 (2015).
Lassalle, F. et al. PLoS. Genet. 11, e1004941 (2015).
Lynch, M. Proc. Natl Acad. Sci. USA 109, 18851–18856 (2012).
Long, H. et al. Genome Biol. Evol. 8, 3815–3821 (2016).
McKenna, A. et al. Genome Res. 20, 1297–1303 (2010).
DePristo, M. A. et al. Nat. Genet. 43, 491–498 (2011).
Van der Auwera, G. A. et al. Curr. Protoc. Bioinformatics 43, 11.10.1–33 (2013).
Thorvaldsdottir, H., Robinson, J. T. & Mesirov, J. P. Brief. Bioinform. 14, 178–192 (2013).
Support was provided by the Multidisciplinary University Research Initiative awards W911NF-09-1-0444 and W911NF-14-1-0411 from the US Army Research Office to M.L., National Institutes of Health awards R01-GM036827 and R35-GM122566 to M.L., National Natural Science Foundation of China 31741071 to H.L., R01-GM51986 and R35-GM122556 to Y.V.B., F32-GM083581 to D.T.K. and National Science Foundation grant DOB 1442246 to J.T.L. We thank T. G. Doak, P. Keightley, K. Morris, R. Ness, I. Ruiz-Trillo, S. Simpson, W. K. Thomas, A. Uchimura and Z. Ye for providing strains and/or technical help in data acquisition. We thank L. Duret for helpful comments.
The authors declare no competing financial interests.
Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Table 1 shows details of mutation datasets used in this study. Tables 2 and 3 list mutations from 12 microbial organisms that are first reported here. Table 4 shows results of linear regressions involving genome G/C content vs the expectations based on mutation bias. Table 5 gives parameters from regressions of selection coefficients at different genomic sites, as well as those of selection coefficients vs the mutation bias m. Table 6 shows the relationship between expression level and G/C content within 17 organisms.
About this article
Cite this article
Long, H., Sung, W., Kucukyildirim, S. et al. Evolutionary determinants of genome-wide nucleotide composition. Nat Ecol Evol 2, 237–240 (2018). https://doi.org/10.1038/s41559-017-0425-y
Physicochemical Foundations of Life that Direct Evolution: Chance and Natural Selection are not Evolutionary Driving Forces
Molecular Biology and Evolution (2020)
Ancestral niche separation and evolutionary rate differentiation between sister marine flavobacteria lineages
Environmental Microbiology (2020)
The ISME Journal (2020)
Genome Biology and Evolution (2020)