We developed a high-throughput, unbiased strategy for the identification of endogenous biomolecular condensates by merging cell volume compression, sucrose density gradient centrifugation and quantitative mass spectrometry. We demonstrated the performance of this strategy by identifying both global condensate proteins and those responding to specific biological processes on a proteome-wide scale.
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References
Alberti, S. & Hyman, A. A. Biomolecular condensates at the nexus of cellular stress, protein aggregation disease and ageing. Nat. Rev. Mol. Cell Bio. 22, 196–213 (2021). A review article that presents the role of condensates in age-related human disease.
You, K. et al. PhaSepDB: a database of liquid–liquid phase separation related proteins. Nucleic Acids Res. 48, D354–D359 (2020). This paper reports a database of liquid–liquid phase separation-related proteins.
Alberti, S., Gladfelter, A. & Mittag, T. Considerations and challenges in studying liquid-liquid phase separation and biomolecular condensates. Cell 176, 419–434 (2019). A review article that presents guidelines for the study of biomolecular condensates.
Riback, J. A. et al. Composition-dependent thermodynamics of intracellular phase separation. Nature 581, 209–214 (2020). This paper reports the composition of condensates is tuned by thermodynamics.
Li, Y., Tang, W. & Guo, M. The cell as matter: Connecting molecular biology to cellular functions. Matter 4, 1863–1891 (2021). A review article that presents the relationship between intracellular biomolecular processes and Multicellular tissue behaviour.
Li, Y. et al. Volumetric compression induces intracellular crowding to control intestinal organoid growth via Wnt/β-catenin signaling. Cell Stem Cell 28, 63–78 (2021). This paper reports that cell volume compression results in rapid and reversible membraneless organelles.
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This is a summary of: Li, P. et al. High-throughput and proteome-wide discovery of endogenous biomolecular condensates. Nat. Chem. https://doi.org/10.1038/s41557-024-01485-1 (2024).
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Identifying phase-separating biomolecular condensates in cells. Nat. Chem. (2024). https://doi.org/10.1038/s41557-024-01503-2
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DOI: https://doi.org/10.1038/s41557-024-01503-2
