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Designing in vivo active DNAzymes

The therapeutic applications of DNAzymes are limited because of their low effectiveness in vivo. Now, a promising approach for constructing DNAzymes that show high gene-silencing efficiency in mammalian cells has been developed. This approach incorporates chemical modifications into an existing DNAzyme scaffold.

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Fig. 1: Design of DNAzymes for efficient gene silencing in vivo.


  1. Roberts, T. C., Langer, R. & Wood, M. J. A. Nat. Rev. Drug Discov. 19, 673–694 (2020).

    Article  CAS  Google Scholar 

  2. Silverman, S. K. Trends Biochem. Sci. 41, 595–609 (2016).

    Article  CAS  Google Scholar 

  3. Wang, Y., Nguyen, K., Spitale, R. C. & Chaput, J. C. Nat. Chem. (2021).

    Article  PubMed  Google Scholar 

  4. Breaker, R. R. & Joyce, G. F. Cell Chem. Biol. 1, 223–229 (1994).

    CAS  Google Scholar 

  5. Santoro, S. W. & Joyce, G. F. Proc. Natl Acad. Sci. USA 94, 4262–4266 (1997).

    Article  CAS  Google Scholar 

  6. Hollenstein, M. Curr. Opin. Chem. Biol. 52, 93–101 (2019).

    Article  CAS  Google Scholar 

  7. Schubert, S. et al. Nucleic Acids Res. 31, 5982–5992 (2003).

    Article  CAS  Google Scholar 

  8. Fokina, A. A., Meschaninova, M. I., Durfort, T., Venyaminova, A. G. & François, J.-C. Biochemistry 51, 2181–2191 (2012).

    Article  CAS  Google Scholar 

  9. Damha, M. J. et al. J. Am. Chem. Soc. 120, 12976–12977 (1998).

    Article  CAS  Google Scholar 

  10. Chaput, J. C. Acc. Chem. Res. 54, 1056–1065 (2021).

    Article  CAS  Google Scholar 

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Correspondence to Chuanzheng Zhou.

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Zhou, Y., Zhou, C. Designing in vivo active DNAzymes. Nat. Chem. 13, 299–301 (2021).

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