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SYNTHETIC BIOLOGY

Optimizing orthogonality

Nature Chemistry volume 10, pages 802–803 (2018)Cite this article

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A new pyrrolysyl-tRNA synthetase/PyltRNA (PylRS/PyltRNA) pair that is mutually orthogonal to existing PylRS/PyltRNA pairs has now been discovered and optimized. This system could enable the site-specific incorporation of a greater number of distinct non-canonical amino acids into a protein.

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Fig. 1: Discovery and optimization of a new orthogonal PylRS/PyltRNA pair.

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Authors and Affiliations

  1. The Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing, China

    William S. C. Ngai & Peng R. Chen

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  1. William S. C. Ngai
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  2. Peng R. Chen
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Correspondence to Peng R. Chen.

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Ngai, W.S.C., Chen, P.R. Optimizing orthogonality. Nature Chem 10, 802–803 (2018). https://doi.org/10.1038/s41557-018-0115-7

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