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Synergistic interplay of ABA and BR signal in regulating plant growth and adaptation

Nature Plants volume 7, pages 1108–1118 (2021)Cite this article

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Abstract

Complex antagonistic interactions between abscisic acid (ABA) and brassinosteroid (BR) signalling pathways have been widely documented. However, whether or how ABA interacts synergistically with BR in plants remains to be elucidated. Here, we report that low, but not high, concentration of ABA increases lamina joint inclination of rice seedling, which requires functional BR biosynthesis and signalling. Transcriptome analyses confirm that about 60% of low-concentration ABA early response genes can be regulated by BR in the same directions. ABA activates BR signal in a fast, limited and short-term manner and the BR-biosynthesis regulatory gene, OsGSR1, plays a key role during this process, whose expression is induced slightly by ABA through transcriptional factor ABI3. Moreover, the early short-term BR signal activation is also important for ABA-mediated salt stress tolerance. Intriguingly, the process and effect of short-term BR signal activation were covered by high concentration of ABA, implying adaptive mechanisms existed in plants to cope with varying degrees of stress.

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Fig. 1: Low concentration of ABA increases LJI of rice seedling.
Fig. 2: ABA regulates LJI through activating BR signalling.
Fig. 3: Synergistic response of rice seedlings to ABA and BL at the transcriptional level.
Fig. 4: OsGSR1 is required for ABA-regulated LJI in rice seedling.
Fig. 5: Transactivation of OsGSR1 by ABI3 is required for ABA-mediated LJI.
Fig. 6: ABA-mediated salt stress tolerance depends on transient accumulation of BR.
Fig. 7: A working model of complex interplay between ABA and BR signalling in rice seedlings.

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Data availability

Genome sequence data from this study can be found in the GenBank/EMBL libraries under the following accession numbers: Os01g0911700 (ABI3), Os01g0859300 (ABI5), Os04g0448900 (ABA1), Os01g0197100 (D2), Os04g0469800 (D11), Os06g0266800 (OsGSR1) and Os07g0569100 (OsREM4.1). O. sativa proteome sequences were downloaded from IRGSP (http://rice.plantbiology.msu.edu/). The RNA-seq data have been deposited in Sequence Read Archive of NCBI (https://trace.ncbi.nlm.nih.gov/Traces/sra/sra.cgi?) under accession number PRJNA732471. The data supporting the findings of this study are available within the article and its supplementary data, or from the corresponding authors upon reasonable request. Source data are provided with this paper.

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Acknowledgements

We thank J. Fang, H. Tong and Y. Xiao for providing the seeds of ABA and BR-related mutants. We also thank G. Li for performing field cultivation and S. Cao for performing rice transformation. This work was supported by the National Natural Science Foundation of China (grant nos. 31771360 and 32070547).

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Authors and Affiliations

  1. State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, and the Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, China

    Qianqian Li, Fan Xu, Zhuo Chen, Zhenfeng Teng, Kai Sun, Xiancai Li, Jianyuan Yu, Guoxia Zhang, Yan Liang, Lin Du, Chengcai Chu & Jiuyou Tang

  2. College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, China

    Qianqian Li, Zhenfeng Teng, Kai Sun, Jianyuan Yu, Yan Liang & Chengcai Chu

  3. State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China

    Xiahe Huang & Yingchun Wang

  4. Biogle Genome Editing Center, Changzhou, China

    Yangwen Qian

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Contributions

J.T. and C.C. conceived the project, designed the experiments and analysed the data. Q.L. and J.T. performed most of the experiments with the help of F.X., Z.C., K.S., Y.L., Z.T, J.Y., G.Z., X.L., X.H., L.D., Y.Q. and Y.W. J.T. wrote the manuscript. C.C. and Q.L. revised the manuscript with contributions from all authors.

Corresponding authors

Correspondence to Chengcai Chu or Jiuyou Tang.

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The authors declare no competing interests.

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Peer review information Nature Plants thanks Qiaoquan Liu, Brigitte Poppenberger and Hojin Ryu for their contribution to the peer review of this work.

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Supplementary information

Supplementary Information

Supplementary Figs. 1–10.

Reporting Summary

Supplementary Table 1

The genes regulated by ABA or BL at each time point (log2 ≥ 0.5 or ≤ –0.5 and Padj ≤ 0.05). A, ABA treatment; B, BL treatement. 0.5, 1, 2 and 4 indicate 0.5, 1, 2 and 4 hours after ABA or BL treatments, respectively.

Supplementary Table 2

Genome-wide transcription profiles of gsr1-1, abi3 and dlt. log2 ≥ 0.5 or ≤ −0.5 and Padj ≤ 0.01.

Supplementary Table 3

Primer pairs used in this study.

Source data

Source Data Fig. 2

Original western blots.

Source Data Fig. 5

Original EMSA PAGE.

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Li, Q., Xu, F., Chen, Z. et al. Synergistic interplay of ABA and BR signal in regulating plant growth and adaptation. Nat. Plants 7, 1108–1118 (2021). https://doi.org/10.1038/s41477-021-00959-1

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