Abstract
Anther dehiscence determines successful sexual reproduction of flowering plants through timely release of pollen grains for pollination and fertilization. Downregulation of auxin levels during pollen mitosis is essential for promoting anther dehiscence along with pollen maturation. How this key transition of auxin levels is regulated in male organs remains elusive. Here, we report that the rice FT-INTERACTING PROTEIN 7 is highly expressed in anthers before pollen mitotic divisions and facilitates nuclear translocation of a homeodomain transcription factor, Oryza sativa homeobox 1, which directly suppresses a predominant auxin biosynthetic gene, OsYUCCA4, during the late development of anthers. This confers a key switch of auxin levels between meiosis of microspore mother cells and pollen mitotic divisions, thus controlling the timing of anther dehiscence during rice anthesis. Our findings shed light on the mechanism of hormonal control of anther dehiscence, and provide a new avenue for creating hormone-sensitive male sterile lines for hybrid plant breeding.
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Change history
02 November 2018
In the version of this Article originally published, an incorrect immunoblot was used in the left, middle panel of Fig. 6a, which describes α-tubulin expression in whole-cell extracts. This panel, and the corresponding quantitative result shown in Supplementary Fig. 11e, have now been corrected in the Article. This does not affect the results or conclusions of this work.
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Acknowledgements
We thank Z. He and J.-K. Zhu for providing the BAC clone and psgR-CAS9-Os vector, and members of the Yu lab for discussion and comments on the manuscript. This work was supported by the Singapore National Research Foundation Investigatorship Programme (NRF-NRFI2016-02), the National Natural Science Foundation of China (grant no. 31529001), the Ministry of Education and Bureau of Foreign Experts of China (grant B14027), and the intramural research support from National University of Singapore and Temasek Life Sciences Laboratory.
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S.S., Y.C. and H.Y. conceived the project; S.S. and Y.C. performed most of the experiments; L.L. participated in investigating the link between OsFTIP7 and OSH1; Y.H.B.S. conducted part of the rice transformation. S.S. and Y.C. conducted all statistical analyses; S.S., Y.C., C.M., Y.G. and H.Y. analysed data; S.S., Y.C. and H.Y. wrote the paper.
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Song, S., Chen, Y., Liu, L. et al. OsFTIP7 determines auxin-mediated anther dehiscence in rice. Nature Plants 4, 495–504 (2018). https://doi.org/10.1038/s41477-018-0175-0
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DOI: https://doi.org/10.1038/s41477-018-0175-0
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