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Retinal haemorrhages on ultra-widefield red channel images and perfusion status in central retinal vein occlusion

Eye volume 37, pages 2305–2309 (2023)Cite this article

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Abstract

Purpose

To evaluate the relationship between retinal haemorrhages detected on Ultra-widefield (UWF) red channel images and perfusion status in eyes with acute central retinal vein occlusion (CRVO).

Methods

UWF fundus images were split into green and red channels using ImageJ software. The retinal haemorrhages were calculated quantitatively with both the green and red channel images, resulting in green channel haemorrhages (GCH) and red channel haemorrhages (RCH). The nonperfusion area (NPA) was also calculated from fluorescein angiography in each eye. The relationships between both the GCH and RCH with the NPA were investigated.

Results

Thirty-two eyes of 32 patients with acute CRVO (18 men, 14 women) were included. The mean GCH and RCH values were 10.4% ± 8.2% and 1.7% ± 1.7%, respectively. The mean NPA was 39.2% ± 28.8%. Significant correlations were seen between the GCH and NPA (r = 0.38; P = 0.022) and RCH and NPA (r = 0.44; P = 0.010, linear regression analysis). Multivariate analysis suggested that only the RCHs were correlated significantly with the NPA.

Conclusions

Retinal haemorrhages detected by UWF red channel imaging were less compared to green channel imaging and associated closely with retinal NPAs in eyes with acute CRVO.

UWF red channel imaging allowed us to identify ischaemia-related haemorrhage.

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Fig. 1: Methods for quantification of green and red channel retinal haemorrhages.
Fig. 2: Correlation between the RCHs, GCHs, and NPA.

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author (ST) on reasonable request.

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Author notes

  1. These authors contributed equally: Shin Tanaka, Yui Tanaka.

Authors and Affiliations

  1. Department of Ophthalmology and Micro-Technology, Yokohama City University, Yokohama, Kanagawa, Japan

    Shin Tanaka, Yui Tanaka, Tatsuya Inoue, Koichi Nagura, Rei Arasaki, Kazuyoshi Okawa, Shohei Kitahata, Kentaro Nakamura, Shiro Kastube, Yasuo Yanagi, Maiko Maruyama-Inoue & Kazuaki Kadonosono

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  1. Shin Tanaka
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Contributions

ST, YT and TI were responsible for writing the report, conducting the search, extracting and analysing data, interpreting results, updating reference lists. KoN, RA, KO, ShoK, KeN and ShiK contributed to extracting data. YY, MI and KK provided feedback on the report.

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Correspondence to Shin Tanaka.

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Tanaka, S., Tanaka, Y., Inoue, T. et al. Retinal haemorrhages on ultra-widefield red channel images and perfusion status in central retinal vein occlusion. Eye 37, 2305–2309 (2023). https://doi.org/10.1038/s41433-022-02337-3

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