Arrhythmogenic right ventricular cardiomyopathy (ARVC) is a rare inherited heart-muscle disorder, which is the most common cause of life-threatening arrhythmias and sudden cardiac death (SCD) in young adults and athletes. Early and accurate diagnosis can be crucial in effective ARVC management and prevention of SCD.
The genome Aggregation Database (gnomAD) population of 138,632 unrelated individuals was searched for previously identified ARVC variants, classified as pathogenic or unknown on the disease genetic variant database (http://www.arvcdatabase.info/), in five most-commonly mutated genes: PKP2, DSP, DSG2, DSC2 and JUP, where variants account for 40–50% of all the ARVC cases. Minor allele frequency (MAF) of 0.001 was used to define variants as rare or common.
The gnomAD data contained 117/364 (32%) of the previously reported pathogenic and 152/266 (57%) of the unknown ARVC variants. The cross-ethnic analysis of MAF revealed that 11 previously classified pathogenic and 57 unknown variants were common (MAF ≥ 0.001) in at least one ethnic gnomAD population and therefore unlikely to be ARVC causing.
After applying our MAF analysis the overall frequency of pathogenic ARVC variants in gnomAD was one in 257 individuals, but a more stringent cut-off (MAF ≥ 0.0001) gave a frequency of one in 845, closer to the estimated phenotypic frequency of the disease.
Our study demonstrates that the analysis of large cross-ethnic population sequencing data can significantly improve disease variant interpretation. Higher than expected frequency of ARVC variants suggests that a proportion of ARVC-causing variants may be inaccurately classified, implying reduced penetrance of some variants, and/or a polygenic aetiology of ARVC.
Arrhythmogenic right ventricular cardiomyopathy (ARVC) is a heart muscle disorder that predominantly develops in the right ventricle, due to progressive replacement of myocardium by fatty or fibrofatty tissue leading to conduction disturbances and arrhythmias [1,2,3]. It is a rare disease with an estimated prevalence of 1 in 1000 to 1 in 5000 in the general population [4, 5], and accounts for about 11–22% of sudden cardiac death (SCD) cases among young athletes [6,7,8].
The variants detection rate in individuals who fulfil the Task Force diagnostic criteria  can reach 60% , with variants in desmosomal genes accounting for ~40–50% of cases . The majority of the detected causal variants being located in the PKP2 gene (~45–73% of all causal variants), which encodes Plakophilin-2 [12, 13]. Other commonly mutated genes in ARVC include DSG2 (Desmoglein 2), DSP (Desmoplakin), DSC2 (Desmocollin 2) and JUP (Junction Plakoglobin), which cause the disease mainly in the autosomal dominant mode, apart from the JUP gene and sometimes DSP, DSC2 and more recently DSG2 genes, where variants cause recessive ARVC [14,15,16].
Early diagnosis of ARVC based on clinical criteria remains challenging as SCD can present as either the first or final sign . Genetic testing for ARVC variants offers the opportunity to identify individuals-at-risk in advance, before the clinical symptoms emerge. Next-generation sequencing (NGS) methods provide cost- and time-efficient genetic diagnosis, such as the recently developed genetic test for numerous inherited cardiovascular diseases, including ARVC . However, in many cases difficulties in the interpretation of identified variants, mainly due to an insufficient evidence supporting their pathogenic effect, hinders accurate diagnosis. In 2015, the American College of Medical Genetics together with the Association of Molecular Pathology (ACMG–AMP) published guidelines to assist genetic diagnostic labs in evaluating the pathogenicity of variants . The analysis of variant frequency in population data is the first ACMG–AMP criterion to be included in the variant interpretation process. Recent findings of the 1000 Genomes project based on a multi-ethnic whole-genome analysis strongly suggested that there is a bias in genetic studies of disease-associated variants , with the majority of variants being discovered in Caucasian populations. However, variants that appear to have a low frequency in Caucasians, which often supports their deleterious effect, can be common in other understudied ethnically diverse populations. Unless those have an advantageous effect, such as the sickle cell anaemia variants protecting from malaria infections , they are unlikely to be disease causing, due to negative selection. Although the efficacy of purifying selection may be affected by demographic history of a specific population, several studies demonstrated that this is less likely to have an effect on deleterious variants and that the selection is equally effective across human populations [22,23,24]. Therefore, examining rare allele frequencies across different ethnic groups can provide new insights into the pathogenicity status of some disease-associated variants. The importance of such analysis for correct disease diagnosis has been highlighted in a study of hypertrophic cardiomyopathy patients .
In 2013, Andreasen et al. attempted to examine the frequency of previously published ARVC variants using the NHLBI-Go Exome Sequencing Project (ESP, http://evs.gs.washington.edu/EVS/), which represents whole-exome sequencing data for 6500 individuals of two ethnic groups: European Americans and African Americans . The observed genotype prevalence of ARVC variants in that report was one in five, which was at least 200-fold higher than the estimated phenotype prevalence of the disease. The authors suggested that such inflated ARVC variant frequency in the NHLBI-Go ESP cohort is likely to be due to incorrect classification of some previously published ARVC variants.
The frequency of PKP2, DSG2, DSP and DSC2 variants was also evaluated, in 2016, in the Exome Aggregation Consortium (ExAC) data, a ten time larger whole-exome sequencing data set , however the variant classification was based on an in-house protocol, and variants of unknown significance were not examined.
In early 2017, genetic data from 123,136 individuals sequenced by whole-exome sequencing and 15,496 individuals sequenced by whole-genome sequencing was made available by the Genome Aggregation Database (gnomAD) investigators (http://gnomad.broadinstitute.org/about) . To date, this is the largest and most comprehensive source of genetic information of individuals of different ethnic backgrounds to date.
The variant frequency analysis in separate ethnic groups as opposed to the global frequency in ExAC has been recently shown to be the most powerful approach for Mendelian disease variant frequency filtering and much more accurate than using the NHLBI-Go ESP cohort . In this study we used the gnomAD population data set of 138,632 unrelated individuals, to investigate the frequency of previously reported ARVC variants in five of the most-commonly mutated ARVC genes, across eight different ethnic groups and in the gnomAD population as a whole. ARVC variants defined as pathogenic (n = 364) or unknown (n = 266) according to the manually curated ARVC genetic variants database maintained by the University Medical Center Groningen, the Netherlands , were analysed. Findings of this study should assist genetic diagnostic laboratories in the ARVC variant classification.
The ARVC/D Genetic Variants Database is a freely available collection of variants associated with ARVC and can be accessed via the link http://www.arvcdatabase.info/ . The database’s variant classification is based on literature (clinical and experimental evidence) and in silico predictions. In this study variants classified as pathogenic and unknown, according to the ARVC genetic variants database curation and individually published classifications, which are not always supported by data from functional assays or family co-segregation, were extracted for the variant frequency analysis. Throughout this manuscript the term “pathogenic” was used as reported by the ARVC genetic variants database, which considers multiple published articles when classifying variants. The database was last updated in February 2015 and accessed in October 2017.
gnomAD population data
The gnomAD database contains whole-exome sequencing data of 123,136 unrelated individuals sequenced by whole-exome sequencing and 15,496 unrelated individuals sequenced by whole-genome sequencing from several large-scale projects (http://gnomad.broadinstitute.org/about) . It is the largest publically available population data set to date. Principal component analysis classified gnomAD participants to seven different ethnic groups: Non-Finnish European (EUR), Finnish (FIN), East Asian (EA), South Asian (SA), Latino (LAT), African (AFR) and Ashkenazi Jewish (AJ). A proportion (N = 3234) of the gnomAD population did not unambiguously cluster with any of the major populations and was classified as Other (OTH), which is likely to include individuals of mixed background.
The data sets generated and analysed during the current study are available in the Open Science Framework repository, https://osf.io/kg4br/#.
The minor allele frequency (MAF) cut-off of 0.001, which is recommended for a dominant disease variant discovery in Mendelian diseases , was used to classify variants as rare (MAF < 0.001), frequency that supports variant’s pathogenic effect, and common (MAF ≥ 0.001), which are unlikely to cause ARVC. The cut-off allows for a higher than the estimated prevalence of ARVC in a general population (1 in 500 individuals, as opposed to 1 in 1000), however previous studies suggested that the disease penetrance can be significantly influenced by gender or environmental factors, such as exercise [31, 32], therefore the higher genotype frequency cut-off used in our study should allow for the possibility of reduced penetrance.
Based on the ARVC/D Genetic Variant Database data and the population frequency data from gnomAD, four classes of ARVC variants were distinguished: pathogenic rare (MAF < 0.001), pathogenic common (MAF ≥ 0.001), unknown rare (MAF < 0.001) and unknown common (MAF ≥ 0.001).
Spectrum of ARVC variants in gnomAD
The ARVC/D Genetic Variants Database listed 364 pathogenic and 266 unknown variants across the five major ARVC genes: PKP2, DSP, DSG2, DSC2 and JUP. The majority of different pathogenic variants were reported in the PKP2 gene (n = 171) (summarised in Table 1).
The mean coverage of the analysed genes on the gnomAD database was as follows: DSP = 83.5×, DSG2 = 73.7×, JUP = 66.9×, DSC2 = 73.0× and PKP2 = 75.7×. Out of the 364 ARVC pathogenic variants 117 (32%) were identified in the gnomAD population, which was 83 variants more than in the previously analysed NHLBI-Go ESP cohort . The number was much higher for the unknown variants where 152/266 (57%) of the variants were found in gnomAD. Previously reported pathogenic variants in PKP2 and DSP were the least represented, PKP2 41/171 (24%) and DSP 21/86 (24%) of the variants found in the gnomAD population. In contrast, the majority 10/15 (67%) of the previously reported pathogenic JUP variants were present in the gnomAD population (Table 1). All ARVC-associated variants identified in gnomAD had passed the variant call quality threshold (PASS filter) as set by the gnomAD consortium .
The gnomAD database contained in total 8407 different variants (including common variants located in the untranslated regions, captured intronic and synonymous changes) located in the five ARVC genes, with PKP2 having the highest number of variants per amino acid (1.60), and DSP being at the opposite end with 1.06 variants per amino acid (Table 1).
Cross-ethnic ARVC variants frequency comparison
Frequencies of pathogenic and unknown variants were compared across seven ethnic groups (EUR, FIN, EA, SA, LAT, AFR and AJ) and the OTH group in gnomAD. The analysis showed that 11 out of 117 (9.4%) pathogenic variants located in the five ARVC genes occur at a higher frequency, i.e. are common (with MAF ≥ 0.001) in at least one ethnic group (Fig. 1 and Supplementary Table S1). However, only one pathogenic variant would be classified as common if the overall gnomAD frequency was used for the analysis. The highest proportion of these pathogenic common variants was found in PKP2 (five variants) and DSP (three variants) genes.
The MAF ≥ 0.001 threshold classified 57 unknown variants as common, however 35 of them would still be rare if only the overall gnomAD frequency was taken into consideration. The percentage of unknown pathogenicity variants that were common in at least one of the analysed populations was higher in comparison to the pathogenic variants (34.2% vs. 9.4%). The highest proportion of unknown variants that could be classified as common, i.e. unlikely to have a causative effect, was found in DSC2 followed by DSP (45% and 43%, respectively, of the identified unknown variants), whereas the lowest proportion of such variants was found in the JUP gene (one variant). Details of the unknown variants classified as common in at least one of the analysed ethnic populations are shown in Supplementary Table 2.
Predictive analysis of common pathogenic variants
Variants previously classified as pathogenic that had MAF ≥ 0.001 in at least one of the gnomAD populations were further analysed using two in silico predictive algorithms (PolyPhen-2 , SIFT  and Clinvar database ). Only three of these variants, the DSP (c.2360 A > G) p.(Tyr787Cys), PKP2 (c.176 A > T) p.(Gln59Leu) and JUP (c.1807G > T) p.(Val603Leu) had consistent prediction of being pathogenic according to PolyPhen-2 and SIFT, however, the ClinVar classification was either inconclusive or not available (Table S1). On the other hand, there were two variants in PKP2: (c.2431 C > A) p.(Arg811Ser), (c.1093 A > G) p.(Met365Val), one in DSC2 (c.304 G > A) p.(Glu102Lys), and one in DSP (c.688 G > A p.(Asp230Asn) that were predicted as benign or tolerated by both in silico tools, and had conflicting interpretations as reported on ClinVar (Table S1).
There were 57 unknown common variants, of which 20 were predicted to be benign/tolerated by both PolyPhen-2 and SIFT. The variants are indicated in Supplementary Table S2.
Overall frequency of ARVC variants
The overall ARVC genotype prevalence in the gnomAD population was established after excluding all unknown variants. Since there were no homozygous rare pathogenic variants found in the JUP gene, where variants are known to cause a recessive form of ARVC, and the possibility of identifying compound heterozygotes is not available in the gnomAD data set, JUP variants were also excluded (although there were 81 carriers of a rare pathogenic allele in the JUP gene, assuming that there were no compound heterozygotes). The pathogenic variants were classified as common or rare according to their frequency in the overall (global) gnomAD population and when each ethnic group was considered separately. Table 2 compares the number of alleles present in gnomAD between the two frequency analysis approaches.
When applying the MAF cut-off of 0.001 to separate the rare from common variants, there are over three times more rare pathogenic alleles when using the global gnomAD frequency as opposed to looking at the ethnic groups separately (1646 vs. 539 variants) (Table 2A).
The number of rare pathogenic alleles across all the ethnic groups gave the overall prevalence of ARVC variants of one in 257 individuals (539 variants in 138,632 individuals). However, when applying a more rigorous MAF cut-off of 0.0001, which may be more appropriate for highly heterogeneous and penetrant diseases such as ARVC, as recently proposed by Whiffin et al. , the number of rare pathogenic alleles decreased to 164 (Table 2B). This gave the ARVC prevalence of one in 845 individuals, which is much closer to the observed 1 in 1000, and over 160 times lower than previously estimated in the smaller NHLBI-Go ESP cohort.
New ARVC-associated variants
Since the ARVC genetic variants database was not updated after 2015, we reviewed recent literature (from 2015 until February 2018) using PubMed search terms ‘ARVC’, ‘mutation’ and ‘variant’. Eighty-nine variants that were not listed on the ARVC genetic variants database in the five ARVC genes were identified (Supplementary Table S3). Out of those novel variants 29 were found in the gnomAD population, however, none of the variants had MAF above the 0.001 cut-off in any of the gnomAD’s ethnic sub-groups. This suggests that researchers are becoming more aware of the importance of assessing variant frequency in large population data sets.
NGS has dramatically advanced the process of genetic variant identification; however, the clinical interpretation of identified variants remains a challenge. As more sequencing data are being generated this gives an opportunity to assess the frequency of a variant in a large and multi-ethnic cohort in order to assist the variant prediction process.
In this study, we used the largest available whole-exome/genome sequencing data set and analysed allele frequencies in each of the ethnic groups separately to review previously reported ARVC variants. This allowed us to assess the frequency of additional 83 (and 29 recently published, i.e. not included on the disease genetic variants database) ARVC-associated variants than in the previous study . Pathogenic variants in PKP2 and DSP genes were the least represented in gnomAD, which could suggest that variants in these genes have more deleterious effect in comparison to the remaining ARVC genes and are diluted by natural selection.
We demonstrated the importance of looking at the variant frequency per ethnic group as opposed to the overall gnomAD frequency. Such analysis pointed out 10 pathogenic variants that had MAF < 0.001 in the gnomAD population as a whole, but were common in at least one of the ethnic groups when analysed on group-by-group basis. Interestingly, one of the classified as common pathogenic variants, the DSG2 (c.1003 A > G) p.(Thr335Ala), was recently identified in a homozygous form as a cause of recessive ARVC . Furthermore, a more stringent MAF cut-off of 0.0001 (similar to the one recently suggested for hypertrophic cardiomyopathy ) would reclassify further 32 pathogenic variants as common.
We suggest that the status of variants previously classified as pathogenic that occur at a frequency higher than 0.001 in at least one of the gnomAD populations and that are predicted to be benign by in silico predictive algorithms is reviewed.
The analysis of the ARVC variant prevalence in gnomAD show that the ARVC-causing genotype was still higher than the observed disease frequency (one in 845 when MAF threshold of 0.0001 was used, or even higher, on in 257 when MAF was set to 0.001). This suggests that still a number of variants classified as pathogenic are not truly disease causing or are not fully penetrant.
There is also a possibility that some variants at a higher frequency may act as disease modifiers rather than causal variants, therefore may still be important for the phenotype interpretation. However, this requires further investigations involving deep-phenotyping and DNA-phenotype correlation analysis.
Our study summarises the evidence for variant interpretation process using three important criteria as listed by the ACMG–AMP guidelines: analysis of the variant frequency in the largest available population database, summary of gene-specific database for each variant (ClinVar) and results of the computational (in silico) predictions (PolyPhen2 and SIFT). These findings will be uploaded on to the ARVC genetic variants database (http://www.arvcdatabase.info/) with the next update.
ARVC has been reported across different ethnic populations, however, there is no evidence that its prevalence varies. Nevertheless, since environmental factors, which are likely to differ between some populations, can influence the ARVC phenotype expression, we cannot exclude the possibility that the disease prevalence also differs between some ethnic groups.
There is a chance that the ARVC genotype prevalence in gnomAD is slightly inflated since the analysis assume that each variant is found on a different allele (i.e. in separate individuals).
Hughes SE, McKenna WJ. New insights into the pathology of inherited cardiomyopathy. Heart . 2005;91:257–64.
Meyer S, van der Meer P, van Tintelen JP, van den Berg MP. Sex differences in cardiomyopathies. Eur J Heart Fail. 2014;16:238–47.
Philips B, Cheng A. 2015 update on the diagnosis and management of arrhythmogenic right ventricular cardiomyopathy. Curr Opin Cardiol. 2016;31:46–56.
Sen-Chowdhry S, Morgan RD, Chambers JC, McKenna WJ. Arrhythmogenic cardiomyopathy: etiology, diagnosis, and treatment. Annu Rev Med. 2010;61:233–53.
Basso C, Corrado D, Marcus FI, Nava A, Thiene G. Arrhythmogenic right ventricular cardiomyopathy. Lancet . 2009;373:1289–300.
Dalal D, Nasir K, Bomma C, et al. Arrhythmogenic right ventricular dysplasia: a United States experience. Circulation . 2005;112:3823–32.
Corrado D, Basso C, Schiavon M, Thiene G. Screening for hypertrophic cardiomyopathy in young athletes. N Engl J Med. 1998;339:364–9.
Corrado D, Fontaine G, Marcus FI, et al. Arrhythmogenic right ventricular dysplasia/cardiomyopathy: need for an international registry. Study Group on Arrhythmogenic Right Ventricular Dysplasia/Cardiomyopathy of the Working Groups on Myocardial and Pericardial Disease and Arrhythmias of the European Society of Cardiology and of the Scientific Council on Cardiomyopathies of the World Heart Federation. Circulation . 2000;101:E101–6.
Marcus FI, McKenna WJ, Sherrill D, et al. Diagnosis of arrhythmogenic right ventricular cardiomyopathy/dysplasia: proposed modification of the task force criteria. Circulation . 2010;121:1533–41.
Groeneweg JA, Bhonsale A, James CA, et al. Clinical presentation, long-term follow-up, and outcomes of 1001 arrhythmogenic right ventricular dysplasia/cardiomyopathy patients and family members. Circ Cardiovasc Genet. 2015;8:437–46.
Sen-Chowdhry S, Syrris P, McKenna WJ. Role of genetic analysis in the management of patients with arrhythmogenic right ventricular dysplasia/cardiomyopathy. J Am Coll Cardiol. 2007;50:1813–21.
Calkins H. Arrhythmogenic right ventricular dysplasia. Curr Probl Cardiol. 2013;38:103–23.
Marcus FI, Edson S, Towbin JA. Genetics of arrhythmogenic right ventricular cardiomyopathy: a practical guide for physicians. J Am Coll Cardiol. 2013;61:1945–8.
Romero J, Mejia-Lopez E, Manrique C, Lucariello R. Arrhythmogenic right ventricular cardiomyopathy (ARVC/D): a systematic literature review. Clin Med Insights Cardiol. 2013;7:97–114.
Qadri S, Anttonen O, Viikila J, et al. Case reports of two pedigrees with recessive arrhythmogenic right ventricular cardiomyopathy associated with homozygous Thr335Ala variant in DSG2. BMC Med Genet. 2017;18:86.
Lorenzon A, Pilichou K, Rigato I, et al. Homozygous desmocollin-2 mutations and arrhythmogenic cardiomyopathy. Am J Cardiol. 2015;116:1245–51.
Poloni G, De Bortoli M, Calore M, Rampazzo A, Lorenzon A. Arrhythmogenic right-ventricular cardiomyopathy: molecular genetics into clinical practice in the era of next generation sequencing. J Cardiovasc Med (Hagerstown). 2016;17:399–407.
Pua CJ, Bhalshankar J, Miao K, et al. Development of a comprehensive sequencing assay for inherited cardiac condition genes. J Cardiovasc Transl Res. 2016;9:3–11.
Richards S, Aziz N, Bale S, et al. Standards and guidelines for the interpretation of sequence variants: a joint consensus recommendation of the American College of Medical Genetics and Genomics and the Association for Molecular Pathology. Genet Med: Off J Am Coll Med Genet. 2015;17:405–24.
Genomes Project C, Auton A, Brooks LD, et al. A global reference for human genetic variation. Nature . 2015;526:68–74.
Cyrklaff M, Sanchez CP, Kilian N, et al. Hemoglobins S and C interfere with actin remodeling in Plasmodium falciparum-infected erythrocytes. Science . 2011;334:1283–6.
Do R, Balick D, Li H, Adzhubei I, Sunyaev S, Reich D. No evidence that selection has been less effective at removing deleterious mutations in Europeans than in Africans. Nat Genet. 2015;47:126–31.
Fu W, Gittelman RM, Bamshad MJ, Akey JM. Characteristics of neutral and deleterious protein-coding variation among individuals and populations. Am J Hum Genet. 2014;95:421–36.
Simons YB, Turchin MC, Pritchard JK, Sella G. The deleterious mutation load is insensitive to recent population history. Nat Genet. 2014;46:220–4.
Manrai AK, Funke BH, Rehm HL, et al. Genetic misdiagnoses and the potential for health disparities. N Engl J Med. 2016;375:655–65.
Andreasen C, Nielsen JB, Refsgaard L, et al. New population-based exome data are questioning the pathogenicity of previously cardiomyopathy-associated genetic variants. Eur J Hum Genet. 2013;21:918–28.
Song W, Gardner SA, Hovhannisyan H, et al. Exploring the landscape of pathogenic genetic variation in the ExAC population database: insights of relevance to variant classification. Genet Med: Off J Am Coll Med Genet. 2016;18:850–4.
Lek M, Karczewski KJ, Minikel EV, et al. Analysis of protein-coding genetic variation in 60,706 humans. Nature . 2016;536:285–91.
Lazzarini E, Jongbloed JD, Pilichou K, et al. The ARVD/C genetic variants database: 2014 update. Hum Mutat. 2015;36:403–10.
Bamshad MJ, Ng SB, Bigham AW, et al. Exome sequencing as a tool for Mendelian disease gene discovery. Nat Rev Genet. 2011;12:745–55.
James CA, Bhonsale A, Tichnell C, et al. Exercise increases age-related penetrance and arrhythmic risk in arrhythmogenic right ventricular dysplasia/cardiomyopathy-associated desmosomal mutation carriers. J Am Coll Cardiol. 2013;62:1290–7.
Dalal D, James C, Devanagondi R, et al. Penetrance of mutations in plakophilin-2 among families with arrhythmogenic right ventricular dysplasia/cardiomyopathy. J Am Coll Cardiol. 2006;48:1416–24.
Adzhubei IA, Schmidt S, Peshkin L, et al. A method and server for predicting damaging missense mutations. Nat Methods. 2010;7:248–9.
Kumar P, Henikoff S, Ng PC. Predicting the effects of coding non-synonymous variants on protein function using the SIFT algorithm. Nat Protoc. 2009;4:1073–81.
Landrum MJ,Lee JM,Benson M, et al. ClinVar: public archive of interpretations of clinically relevant variants. Nucleic Acids Res. 2016;44:D862–8.
Whiffin N, Minikel E, Walsh R, et al. Using high-resolution variant frequencies to empower clinical genome interpretation. Genet Med. 2017;19:1151–8.
The authors would like to thank the Genome Aggregation Database (gnomAD) and the groups that provided exome and genome variant data to this resource. A full list of contributing groups can be found at http://gnomad.broadinstitute.org/about.
Authors CLH and MF are funded by the Fondation Leducq Transatlantic Networks of Excellence Program grant (no. 14 CVD03).
Conflict of interest
The authors declare that they have no conflict of interest.
Electronic supplementary material
About this article
Cite this article
Hall, C.L., Sutanto, H., Dalageorgou, C. et al. Frequency of genetic variants associated with arrhythmogenic right ventricular cardiomyopathy in the genome aggregation database. Eur J Hum Genet 26, 1312–1318 (2018) doi:10.1038/s41431-018-0169-4
Whole-Exome Sequencing Identified a De Novo Mutation of Junction Plakoglobin (p.R577C) in a Chinese Patient with Arrhythmogenic Right Ventricular Cardiomyopathy
BioMed Research International (2019)
Heart Failure Reviews (2019)
Heart Rhythm (2019)
Journal of Clinical Investigation (2019)