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Bone turnover, calcium homeostasis, and vitamin D status in Danish vegans

Abstract

Background/objectives

A vegan diet has been associated with increased bone fracture risk, but the physiology linking nutritional exposure to bone metabolism has only been partially elucidated. This study investigated whether a vegan diet is associated with increased bone turnover and altered calcium homeostasis due to insufficient intake of calcium and vitamin D.

Subjects/methods

Fractionated and total 25-hydroxyvitamin D (25(OH)-D), parathyroid hormone (PTH), calcium, and four bone turnover markers (osteocalcin, N-terminal propeptide of type I procollagen (PINP), bone-specific alkaline phosphatase (BAP), and C-terminal telopeptide of type I collagen (CTX)) were measured in serum from 78 vegans and 77 omnivores.

Results

When adjusting for seasonality and constitutional covariates (age, sex, and body fat percentage) vegans had higher concentrations of PINP (32 [95% CI: 7, 64]%, P = 0.01) and BAP (58 [95% CI: 27, 97]%, P < 0.001) compared to omnivores, whereas CTX (30 [95% CI: −1, 72]%, P = 0.06) and osteocalcin (21.8 [95% CI: −9.3, 63.7]%, P = 0.2) concentrations did not differ between the two groups. Vegans had higher serum PTH concentration (38 [95% CI: 19, 60]%; P < 0.001) and lower 25(OH)-D serum concentration (−33 [95% CI: −45, −19]%; P < 0.001), but similar serum calcium concentration (−1 [95% CI: −3, 1]%, P = 0.18 compared to omnivores.

Conclusions

Vegans have higher levels of circulating bone turnover markers compared to omnivores, which may in the long-term lead to poorer bone health. Differences in dietary habits including intake of vitamin D and calcium may, at least partly, explain the observed differences.

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Acknowledgements

The authors would like to thank A Forman and T Lorentzen for technical assistance, and K Kaadtmann, T Toldsted, P Sandbeck and G Lademann for managerial assistance.

Funding

The Novo Nordisk Foundation Center for Basic Metabolic Research is an independent research centre at the University of Copenhagen partially funded by an unrestricted donation from the Novo Nordisk Foundation (www.metabol.ku.dk). Funders had no influence on study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Author contributions

THH, TH, HV, OP, and KHA designed research; THH, NRJ, and AC conducted research; THH and MTBM analyzed data; THH, MTBM, and KHA wrote the paper; KHA had primary responsibility for final content. All authors read and approved the final manuscript.

Author information

Conflict of interest

The authors declare that they have no conflict of interest.

Correspondence to Tue H. Hansen.

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