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Micrococcus lacusdianchii sp. nov., an attached bacterium inhibited by metabolites from its symbiotic algae

The Journal of Antibiotics volume 77, pages 163–169 (2024)Cite this article

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Abstract

A novel actinobacterial strain, designated as JXJ CY 30 T, was isolated from the phycosphere of Microcystis aeruginosa FACHB-905 (Maf) collected from Lake Dianchi, China. The strain was a Gram-stain-positive, aerobic and coccus-shaped actinobacterium. It had alanine, glutamic acid, aspartic acid, and lysine in the peptidoglycan, and mannose, ribose and arabinose in its cell wall sugars, anteiso-C15:0 and iso-C15:0 as the main cellular fatty acids, MK-7 and MK-8 as the major respiratory quinones, and phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, glycolipid, and an unidentified phospholipid as the polar lipids. The DNA G + C content was 73.08%. Its 16 S rRNA gene sequence shared 99.14%, and 98.75% similarities with Micrococcus flavus DSM 19079 T and M. porci KD337-16T, respectively, and ≤98.41% similarities with other type strains of the genus Micrococcus. It formed independent clade with M. flavus DSM 19079 T on the phylogenetic trees. The digital DNA-DNA hybridization and average nucleotide identity values between strain JXJ CY 30 T and M. flavus DSM 19079 T and M. porci KD337-16T were 48.0% and 92.1%, 25.5% and 83.2%, respectively. These data above indicated that strain JXJ CY 30 T represented a new species of the genus Micrococcus, and the species epithet is proposed as Micrococcus lacusdianchii sp. nov. (type strain JXJ CY 30 T = KCTC 49378 T = CGMCC 1.17508 T). Strain JXJ CY 30 T can potentially provide Maf with various nutrients such as available phosphorus and nitrogen, plant hormones, various vitamins and carotenoids for growth, while it was inhibited by metabolites from its symbiotic algae Maf.

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Acknowledgements

This research was supported by National Natural Science Foundation of China (NO. 32360028), and Programs of the Education Department of Jiangxi Province of China (GJJ211837).

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  1. These authors contributed equally: Le Wang, Yao Xiao

Authors and Affiliations

  1. College of Pharmacy and Life Science, Jiujiang University, Jiujiang, 332005, China

    Le Wang, Yao Xiao, Wenxin Lai, Ru Jia, Qinglin Deng, Xin Wang, Hongqiu Shi, Yiwen Yang & Binghuo Zhang

  2. Jiujiang Key Laboratory for the Development and Utilization of Traditional Chinese Medicine resources in Northwest Jiangxi, Jiujiang, China

    Binghuo Zhang

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Contributions

BZ designed the experiments. LW, YX, WL, RJ, QD and XW performed the experiments. LW, YX, HS, YY and BZ analyzed the data, and drafted and revised the manuscript. All authors reviewed and approved the final version of the paper.

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Correspondence to Yiwen Yang or Binghuo Zhang.

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Wang, L., Xiao, Y., Lai, W. et al. Micrococcus lacusdianchii sp. nov., an attached bacterium inhibited by metabolites from its symbiotic algae. J Antibiot 77, 163–169 (2024). https://doi.org/10.1038/s41429-023-00690-3

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