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Rapid detection of fluoroquinolone resistance in Mycobacterium tuberculosis using a novel multienzyme isothermal rapid assay

The Journal of Antibiotics volume 76, pages 598–602 (2023)Cite this article

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Abstract

Simple, rapid, and accurate detection of Fluoroquinolone (FQ) resistance is essential for early initiation of appropriate anti-tuberculosis treatment regimen among rifampicin-resistant tuberculosis (RR-TB). In this study, we developed a new assay, which combines multienzyme isothermal rapid amplification and a lateral flow strip (MIRA-LF), to identify the mutations on codons 90 and 94 of gyrA for detecting levofloxacin (LFX) resistance. Compared to conventional phenotypic drug susceptibility testing, the new assay detected fluoroquinolone resistance with a sensitivity, specificity, and accuracy of 92.4%, 98.5%, and 96.5%, respectively. Thus, these characteristics of the newly developed MIRA-LF assay make it particularly useful and accurate for detecting FQ resistance in Mycobacterium tuberculosis in resource-limited condition.

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Funding

This study was supported by the projects from National Key Program of Mega Infectious Diseases (Grant No. 2018ZX10302302-001). The funder had no role in the study design, data collection and analysis, decision to publish, or manuscript preparation.

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Author notes

  1. These authors contributed equally: Ma-chao Li, Yao Lu, Hai-can Liu

Authors and Affiliations

  1. State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Beijing, China

    Ma-chao Li, Hai-can Liu, Xiao-tian Nan, Gui-lian Li, Xiu-qin Zhao, Kang-Lin Wan & Li-li Zhao

  2. Department of Laboratory Medicine, Ningbo First Hospital, Ningbo Hospital of Ningbo University, Ningbo, 315010, China

    Yao Lu

  3. College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Shi-qiang Lin

  4. Beijing Center for Disease Control and Prevention, 100013, Beijing, China

    Cheng Qian

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  1. Ma-chao Li
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Corresponding author

Correspondence to Li-li Zhao.

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The authors declare no competing interests.

Ethics approval

This study was approved by the Ethics Committee of the National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention. The patients with TB were included only after we received informed written consent from themselves.

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Li, Mc., Lu, Y., Liu, Hc. et al. Rapid detection of fluoroquinolone resistance in Mycobacterium tuberculosis using a novel multienzyme isothermal rapid assay. J Antibiot 76, 598–602 (2023). https://doi.org/10.1038/s41429-023-00639-6

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