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Cyclic enaminones and a 4-quinazolinone from an unidentified actinomycete of the family Micromonosporaceae

The Journal of Antibiotics volume 75, pages 610–618 (2022)Cite this article

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Abstract

Four novel cyclic enaminones, designated RD4123A−D (1−4), and a new 4-quinazolinone metabolite, RD4123E (5), were isolated from the culture extract of an unidentified actinomycete strain RD004123, which belongs to the family Micromonosporaceae. Structures of 1−5 were determined by spectroscopic analyses using NMR, MS, and electronic circular dichroism (ECD), combined with quantum chemical calculations of ECD and NMR chemical shifts and biosynthetic consideration. Compounds 1−5 showed weak to modest cytotoxicity against murine leukemia P388 cells, while being inactive against bacteria and fungi.

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Acknowledgements

The authors are in debt to Dr. Moriyuki Hamada at Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Chiba, Japan, for acquiring the SEM image, and Prof. Y. Hikichi and Dr. A. Kanda at Kochi University for providing R. solanacearum SUPP1541. This work was supported by JSPS KAKENHI Grant Number 19K05848 to Y.I.. P388 cells were obtained from JCRB Cell Bank under an accession code JCRB0017 (Lot. 06252002).

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  1. Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama, 939-0398, Japan

    Desy W. Triningsih, Tao Zhou, Keisuke Fukaya, Enjuro Harunari, Naoya Oku, Daisuke Urabe & Yasuhiro Igarashi

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  1. Desy W. Triningsih
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Correspondence to Yasuhiro Igarashi.

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Triningsih, D.W., Zhou, T., Fukaya, K. et al. Cyclic enaminones and a 4-quinazolinone from an unidentified actinomycete of the family Micromonosporaceae. J Antibiot 75, 610–618 (2022). https://doi.org/10.1038/s41429-022-00558-y

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