Skip to main content

Thank you for visiting You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Antifungal activity of dehydrocurvularin for Candida spp. through the inhibition of adhesion to human adenocarcinoma cells


Cell adhesion plays a crucial role in candidiasis through invasion of the human body and obtaining resistance to drugs by forming biofilms. Cell adhesion thus is a critical target for combating candidiasis by preventing the entry of fungal hyphae into the epithelium. We report here that dehydrocurvularin (1), isolated from the marine-derived fungus Curvularia aeria, exhibited anti-fungal activities for Candida albicans and Candida auris. This compound also prevented the adherence of C. albicans to human adenocarcinoma cells. Real-time RT-PCR analysis showed that exposure to 1 results in decreased expression of HWP1, EFG1, and ECE1, genes involved in Candida adhesion to epithelial cells and hyphal morphogenesis.

This is a preview of subscription content, access via your institution

Access options

Buy article

Get time limited or full article access on ReadCube.


All prices are NET prices.

Fig. 1
Fig. 2
Fig. 3


  1. Kojic EM, Darouiche RO. Candida infections of medical devices. Clin Microbiol Rev. 2004;17:255–67.

    Article  Google Scholar 

  2. Agnew-Francis KA, Tang Y, Lin X, Low YS, Wun SJ, Kuo A, Elias SMASI, Lonhienne T, Condon ND, Pimentel BNAS, Vergani CE, Smith MT, Fraser JA, Williams CM, Guddat LW. Herbicides that target acetohydroxyacid synthase are potent inhibitors of the growth of drug-resistant Candida auris. ACS Infect Dis. 2020;6:2901–12.

    Article  CAS  Google Scholar 

  3. Sionov RV, Feldman M, Smoum R, Mechoulam R, Steinberg D. Anandamide prevents the adhesion of filamentous Candida albicans to cervical epithelial cells. Sci Rep. 2020;10:13728.

    Article  CAS  Google Scholar 

  4. kobayashi A, Hino T, Yata S, Itoh TJ, Sato H, Kawazu K. Unique spindle poisons, curvularin and its derivatives, isolated from Penicillium species. Agic Biol Chem. 1988;52:3119–23.

    CAS  Google Scholar 

  5. Xie LW, Ouyang YC, Zou K, Wang GH, Chen MJ, Sun HM, Dai SK, Li X. Isolation and difference in anti-Staphylococcus aureus bioactivity of curvularin derivates from fungus Eupenicillium sp. Appl Biochem Biotechnol. 2009;159:284–93.

    Article  CAS  Google Scholar 

  6. CLSI: Reference method for broth dilution anti-fungal susceptibility testing of yeasts: approved standard-second edition (M27-A3) Wayne PA 2002.

  7. Jung P, Mischo CE, Gunaratnam G, Spengler C, Becker SL, Hube B, Jacobs K, Bischoff M. Candida albicans adhesion to central venous catheters: Impact of blood plasma-driven germ tube formation and pathogen-derived adhesins. Virulence. 2020;11:1453–65.

    Article  CAS  Google Scholar 

  8. Watanabe T, Ogasawara A, Mikami T, Matsumoto T. Hyphal formation of Candida albicans is controlled by electron transfer system. Biochem Biophys Res Commun. 2006;348:206–11.

    Article  CAS  Google Scholar 

  9. Kurakado S, Takatori K, Sugita T. Minocycline inhibits Candida albicans budded-to-hyphal-form transition and biofilm formation. Jpn J Infect Dis. 2017;70:490–4.

    Article  CAS  Google Scholar 

  10. Lohse MB, Gulati M, Johnson AD, Nobile CJ. Development and regulation of single- and multi-species Candida albicans biofilms. Nat Rev Microbiol. 2018;16:19–31.

    Article  CAS  Google Scholar 

Download references


This work was supported by JSPS KAKENHI Grant Number JP20K16029 to H.K.

Author information

Authors and Affiliations


Corresponding author

Correspondence to Hitoshi Kamauchi.

Ethics declarations

Conflict of interest

The authors declare no competing interests.

Additional information

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary information

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Kamauchi, H., Furukawa, M., Kiba, Y. et al. Antifungal activity of dehydrocurvularin for Candida spp. through the inhibition of adhesion to human adenocarcinoma cells. J Antibiot 75, 530–533 (2022).

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI:


Quick links