Cell adhesion plays a crucial role in candidiasis through invasion of the human body and obtaining resistance to drugs by forming biofilms. Cell adhesion thus is a critical target for combating candidiasis by preventing the entry of fungal hyphae into the epithelium. We report here that dehydrocurvularin (1), isolated from the marine-derived fungus Curvularia aeria, exhibited anti-fungal activities for Candida albicans and Candida auris. This compound also prevented the adherence of C. albicans to human adenocarcinoma cells. Real-time RT-PCR analysis showed that exposure to 1 results in decreased expression of HWP1, EFG1, and ECE1, genes involved in Candida adhesion to epithelial cells and hyphal morphogenesis.
This is a preview of subscription content, access via your institution
Subscribe to Journal
Get full journal access for 1 year
only $9.92 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Tax calculation will be finalised during checkout.
Get time limited or full article access on ReadCube.
All prices are NET prices.
Kojic EM, Darouiche RO. Candida infections of medical devices. Clin Microbiol Rev. 2004;17:255–67.
Agnew-Francis KA, Tang Y, Lin X, Low YS, Wun SJ, Kuo A, Elias SMASI, Lonhienne T, Condon ND, Pimentel BNAS, Vergani CE, Smith MT, Fraser JA, Williams CM, Guddat LW. Herbicides that target acetohydroxyacid synthase are potent inhibitors of the growth of drug-resistant Candida auris. ACS Infect Dis. 2020;6:2901–12.
Sionov RV, Feldman M, Smoum R, Mechoulam R, Steinberg D. Anandamide prevents the adhesion of filamentous Candida albicans to cervical epithelial cells. Sci Rep. 2020;10:13728.
kobayashi A, Hino T, Yata S, Itoh TJ, Sato H, Kawazu K. Unique spindle poisons, curvularin and its derivatives, isolated from Penicillium species. Agic Biol Chem. 1988;52:3119–23.
Xie LW, Ouyang YC, Zou K, Wang GH, Chen MJ, Sun HM, Dai SK, Li X. Isolation and difference in anti-Staphylococcus aureus bioactivity of curvularin derivates from fungus Eupenicillium sp. Appl Biochem Biotechnol. 2009;159:284–93.
CLSI: Reference method for broth dilution anti-fungal susceptibility testing of yeasts: approved standard-second edition (M27-A3) Wayne PA 2002.
Jung P, Mischo CE, Gunaratnam G, Spengler C, Becker SL, Hube B, Jacobs K, Bischoff M. Candida albicans adhesion to central venous catheters: Impact of blood plasma-driven germ tube formation and pathogen-derived adhesins. Virulence. 2020;11:1453–65.
Watanabe T, Ogasawara A, Mikami T, Matsumoto T. Hyphal formation of Candida albicans is controlled by electron transfer system. Biochem Biophys Res Commun. 2006;348:206–11.
Kurakado S, Takatori K, Sugita T. Minocycline inhibits Candida albicans budded-to-hyphal-form transition and biofilm formation. Jpn J Infect Dis. 2017;70:490–4.
Lohse MB, Gulati M, Johnson AD, Nobile CJ. Development and regulation of single- and multi-species Candida albicans biofilms. Nat Rev Microbiol. 2018;16:19–31.
This work was supported by JSPS KAKENHI Grant Number JP20K16029 to H.K.
Conflict of interest
The authors declare no competing interests.
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Kamauchi, H., Furukawa, M., Kiba, Y. et al. Antifungal activity of dehydrocurvularin for Candida spp. through the inhibition of adhesion to human adenocarcinoma cells. J Antibiot 75, 530–533 (2022). https://doi.org/10.1038/s41429-022-00543-5