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Investigation of antibiotic resistance determinants and virulence factors of uropathogenic Escherichia coli

The Journal of Antibiotics volume 73, pages 314–319 (2020)Cite this article

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Abstract

Multidrug-resistant (MDR) uropathogenic Escherichia coli (UPEC) are prevalent throughout the world resulting in a major public health burden. In this research, we isolated and identified 28 MDR UPEC from one university hospital in China, investigated MDR and pathogenic mechanisms by PCR, including 55 antibiotic resistance determinants (ARDs) genes, 13 genetic markers of mobile genetic elements (MGEs) and 6 virulence factors (VFs) genes. In these isolates, we identified 23 ARDs genes and 6 genetic markers of MGEs that played a key role in MDR phenotypes. In addition, we found 2 VFs genes, hofQ and ompT, which could be associated with pathogenicity and invasiveness of these strains in urinary tract infections (UTIs).

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Acknowledgements

This study is supported by grants from Ningbo Health Branding Subject Fund (PPXK2018–04), Zhejiang Bureau of Traditional Chinese Medicine (2011ZB126), Ningbo Municipal Bureau of Science and Technology (2012A610186).

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Author notes

  1. These authors contributed equally: Ding-he Sun, Ding-feng Lv

Authors and Affiliations

  1. Department of Laboratory Medicine, Ningbo First Hospital, Ningbo, China

    Ding-he Sun, Ding-feng Lv, Li-qing Hu & Xing-bei Weng

  2. Department of Laboratory Medicine, Ningbo Hospital, Zhejiang University School of Medicine, Ningbo, China

    Ding-he Sun, Ding-feng Lv, Li-qing Hu & Xing-bei Weng

  3. Department of Bioinformation, Wuxi Clon-Gen Institute, Wuxi, China

    Zu-huang Mi

  4. Department of Neurosurgery, Ningbo First Hospital, Ningbo, China

    Yi Huang & Xiang Gao

  5. Department of Neurosurgery, Ningbo Hospital, Zhejiang University School of Medicine, Ningbo, China

    Yi Huang & Xiang Gao

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  1. Ding-he Sun
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Correspondence to Xing-bei Weng.

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We obtained the approval letter of Ningbo First Hospital Ethics Committee (Approval No. 2019-R043).

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Sun, Dh., Lv, Df., Mi, Zh. et al. Investigation of antibiotic resistance determinants and virulence factors of uropathogenic Escherichia coli. J Antibiot 73, 314–319 (2020). https://doi.org/10.1038/s41429-020-0284-7

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  • DOI: https://doi.org/10.1038/s41429-020-0284-7

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