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Isolation and characterization of a new cyclic lipopeptide orfamide H from Pseudomonas protegens CHA0

The Journal of Antibiotics volume 73, pages 179–183 (2020)Cite this article

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Abstract

A new cyclic lipopeptide (CLP) orfamide H (1) was purified and identified from the cultural broth of the bacterial strain Pseudomonas protegens CHA0. The crude extract of the strain CHA0 was obtained by an acid-aided precipitation process, then the compound 1 was purified by reversed-phase high-performance liquid chromatography (RP-HPLC). Subsequently, the chemical structure of orfamide H was determined by 1D and 2D nuclear magnetic resonance (NMR) and mass spectrometry (MS). Further biological assays indicate that the new CLP orfamide H shows the activity on inhibiting the appressoria formation of the fungus Magnaporthe oryzae, the causal agent of the blast disease in rice. Taken all together, these results indicated that the new CLP orfamide H has the capacity to be developed as an agrichemical to control blast disease in rice.

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Acknowledgements

Research grants (given to ZM) from the Project for Enhancing the Research Capability of Young Teachers in Northwest Normal University (no. 5007/382) and Gansu Province is acknowledged for supporting this study. The research of JH’s lab was supported by the grant from the National Science Foundation of China (no. 41776178). KS acknowledges the Key Research and Development Project of Gansu Province (no. 18YF1NA051).

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Authors and Affiliations

  1. College of Life Science, Northwest Normal University, 967 East Anning Road, 730070, Lanzhou, China

    Zongwang Ma, Junyu Liang & Kun Sun

  2. Institute of Applied Ecology, Chinese Academy of Sciences, 72 Wenhua Road, 110016, Shenyang, China

    Zongwang Ma & Jiangchun Hu

  3. Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, 1068 Xueyuan Road, 518055, Shenzhen, China

    Songya Zhang

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  1. Zongwang Ma
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  2. Songya Zhang
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Contributions

ZM conceived and designed the study, conducted all the experiments, and wrote the paper. ZM, SZ, JL, KS, and JH together improved the paper and approved the final version of the paper for submission. ZM and SZ revised the paper.

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Correspondence to Zongwang Ma or Jiangchun Hu.

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Ma, Z., Zhang, S., Liang, J. et al. Isolation and characterization of a new cyclic lipopeptide orfamide H from Pseudomonas protegens CHA0. J Antibiot 73, 179–183 (2020). https://doi.org/10.1038/s41429-019-0254-0

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  • DOI: https://doi.org/10.1038/s41429-019-0254-0

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