We have analyzed the expressions of genes which regulate Ras-cAMP-EFG1 and CEK1-MAPK pathways involved in yeast to hyphal form morphogenesis in Candida albicans. The expression profile of genes associated with serum-induced morphogenesis showed reduced expressions of genes involved in these pathways by the treatment with biofabricated silver nanoparticles. Cell elongation gene, ECE1, was downregulated by 5.1 fold by the treatment of silver nanoparticles. Expression of hyphal inducer gene, TEC1 was downregulated by 6.28 fold. Negative regulators of yeast to hyphal transition, TUP1 and RFG1 were downregulated by 2.45 and 5.43 fold, respectively. Current study suggests that silver nanoparticles affect gene expression and may subsequently reduce virulence in C. albicans. Targeting genes involved in virulence may be an acceptable novel treatment strategy for pathogenic fungal infections.
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SDH and S.M.K. are thankful to Prof. PanditVidyasagar, Former Vice Chancellor, SRTM University, Nanded for his kind support under the UGC innovative program. RP is thankful to DST PURSE program and UPE-II program of Department of Biotechnology SavitribaiPhule, Pune University for financial support.
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Halbandge, S.D., Jadhav, A.K., Jangid, P.M. et al. Molecular targets of biofabricated silver nanoparticles in Candida albicans. J Antibiot 72, 640–644 (2019). https://doi.org/10.1038/s41429-019-0185-9
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