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Isolation of depsipeptides and optimization for enhanced production of valinomycin from the North-Western Himalayan cold desert strain Streptomyces lavendulae


Exploration of microbial dynamics of Streptomyces lavendulae ACR-DA1, a psychrotrophic isolate from the North-Western Himalayan cold desert, was carried out using matrix-assisted laser desorbtion ionisation-time of flight mass spectrometer. Valinomycin was found as a major produce and cyclic depsipeptide montanastatin as a minor produce. The yield of the valinomycin was found to be 0.3 mg l−1 in submerged growth condition at the batch scale. Miniaturization of optimization experiments was adept to maximize the production using the expeditious and efficient technique of intact cell mass spectrometry. The present study showed that using optimized conditions and growing the culture in synthetic mineral base starch medium at 10 °C enhanced the production to 19.4 mg l−1. Our results demonstrated 64-fold increase in yield from the wild-type S. lavendulae ACR-DA1 strain using a simple and economical downstream process.

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We express our sincere thanks to Er. Rajneesh Anand for his support in Instrumentation Division. We are indebted to Dr. Inshad Ali Khan, Dr. Shashi Bhushan and their teams for carrying out biological activity. VPS and RS thank CSIR for Research Fellowship. The current work was supported by the funding received from CSIR Mega Lab Project MLP 4010, 4014 and networking project BSC 0117 (Plant-Microbe and Soil Interactions). This manuscript bears institutional publication number IIIM/2153/2017.

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The authors declare that they have no conflict of interest.

Correspondence to Asha Chaubey or Deepika Singh or Ram A. Vishwakarma.

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