Exploration of microbial dynamics of Streptomyces lavendulae ACR-DA1, a psychrotrophic isolate from the North-Western Himalayan cold desert, was carried out using matrix-assisted laser desorbtion ionisation-time of flight mass spectrometer. Valinomycin was found as a major produce and cyclic depsipeptide montanastatin as a minor produce. The yield of the valinomycin was found to be 0.3 mg l−1 in submerged growth condition at the batch scale. Miniaturization of optimization experiments was adept to maximize the production using the expeditious and efficient technique of intact cell mass spectrometry. The present study showed that using optimized conditions and growing the culture in synthetic mineral base starch medium at 10 °C enhanced the production to 19.4 mg l−1. Our results demonstrated 64-fold increase in yield from the wild-type S. lavendulae ACR-DA1 strain using a simple and economical downstream process.
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Hegemann JD, Zimmermann M, Xie X, Marahiel MA. Lasso peptides: an intriguing class of bacterial natural products. Acc Chem Res. 2015;48:1909–19.
Singh VP, et al. Lipovelutibols A-D: cytotoxic lipopeptaibols from the Himalayan cold habitat fungus Trichoderma velutinum. J Nat Prod. 2018;81:219–26.
Brockmann H, Kastner GS. Valinomycin I, XXVII. Msg. about antibiotics from actinomycetes. Chem Ber. 1955;88:57–61.
Goodwin CR, Fenn LS, Derewacz DK, Bachmann BO. Structural mass spectrometry: rapid methods for separation and analysis of peptide natural products. J Nat Prod. 2012;75:48–53.
Sheil MM, Kilby GW, Curtis JM, Bradley CD, Derrick PJ. Low‐energy tandem mass spectra of the cyclic depsipeptide valinomycin - a comparison with four‐sector tandem mass spectra. Org Mass Spectrom. 1993;28:574–6.
Tempelaars MH, Rodrigues S, Abee T. Comparative analysis of antimicrobial activities of valinomycin and cereulide, the Bacillus cereus emetic toxin. Appl Environ Microbiol. 2011;77:2755–62.
Park CN, Lee JM, Lee D, Kim BS. Antifungal activity of valinomycin, a peptide antibiotic produced by Streptomyces sp. strain M10 antagonistic to Botrytis cinerea. J Microbiol Biotechnol. 2008;18:880–4.
Wu CY, et al. Small molecules targeting severe acute respiratory syndrome human coronavirus. Proc Natl Acad Sci USA. 2004;101:10012–7.
Pettit GR, et al. Antineoplastic agents. Part 409: Isolation and structure of montanastatin from a terrestrial actinomycete. Bioorg Med Chem. 1999;7:895–9.
Doi M, Asano A. Montanastatin, cyclo[-(Val-D-Hyv-D-Val-Lac)2−]. Acta Cryst. 2002;E58:o935–o936.
Bundale S, Begde D, Nashikkar N, Kadam T, Upadhyay A. Optimization of culture conditions for production of bioactive metabolites by Streptomyces spp. isolated from soil. Adv Microbiol. 2015;5:441–51.
da Silva IR, Martins MK, Carvalho CM, de Azevedo JL, de Lima, Procópio RE. The effect of varying culture conditions on the production of antibiotics by Streptomyces spp., isolated from the Amazonian soil. Ferment Technol. 2012;1:105.
Li J, Jaitzig J, Hillig F, Sussmuth R, Neubauer P. Enhanced production of the nonribosomal peptide antibiotic valinomycin in Escherichia coli through small-scale high cell density fed-batch cultivation. Appl Microbiol Biotechnol. 2014;98:591–601.
Li J, et al. Type II thioesterase improves heterologous biosynthesis of valinomycin in Escherichia coli. J Biotechnol. 2015;193:16–22.
Sharma R, et al. Revelation and cloning of valinomycin synthetase genes in Streptomyces lavendulae ACR-DA1 and their expression analysis under different fermentation and elicitation conditions. J Biotechnol. 2017;253:40–47.
Bode HB, Bethe B, Hofs R, Zeeck A. Big effects from small changes: possible ways to explore nature’s chemical diversity. Chem Bio Chem. 2002;3:619–27.
Wu C, Choi YH, van Wezel GP. Metabolic profiling as a tool for prioritizing antimicrobial compounds. J Ind Microbiol Biotechnol. 2016;43:299–312.
Matter AM, Hoot SB, Anderson PD, Neves SS, Cheng YQ. Valinomycin biosynthetic gene cluster in Streptomyces: conservation, ecology and evolution. PLoS ONE. 2009;4:e7194.
We express our sincere thanks to Er. Rajneesh Anand for his support in Instrumentation Division. We are indebted to Dr. Inshad Ali Khan, Dr. Shashi Bhushan and their teams for carrying out biological activity. VPS and RS thank CSIR for Research Fellowship. The current work was supported by the funding received from CSIR Mega Lab Project MLP 4010, 4014 and networking project BSC 0117 (Plant-Microbe and Soil Interactions). This manuscript bears institutional publication number IIIM/2153/2017.