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Quellenin, a new anti-Saprolegnia compound isolated from the deep-sea fungus, Aspergillus sp. YK-76

The Journal of Antibioticsvolume 71pages741744 (2018) | Download Citation

Abstract

Saprolegnia parasitica, belonging to oomycetes, is one of virulent pathogen of fishes such as salmon and trout, and causes tremendous damage and losses in commercial aquacultures by saprolegniasis. Previously, malachite green, an effective medicine, had been used to control saprolegniasis. However, this drug has been banned around the world due to its mutagenicity. Therefore, novel anti-saprolegniasis compounds are urgently needed. As a new frontier to discover bioactive compounds, we focused on the deep-sea fungi for the isolation of anti-saprolegniasis compounds. In this paper, on the course of anti-saprolegniasis agents from 546 cultured broths of 91 deep-sea fungal strains, we report a new compound, named quellenin (1) together with three known compounds, diorcinol (2), violaceol-I (3) and violaceol-II (4), from deep-sea fungus Aspergillus sp. YK-76. This strain was isolated from an Osedax sp. annelid, commonly called bone-eating worm, collected at the São Paulo Ridge in off Brazil. Compounds 2, 3 and 4 showed anti-S. parasitica activity. Our results suggest that diorcinol and violaceol analogs and could be good lead candidates for the development of novel agents to prevent saprolegniasis.

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Acknowledgements

We are grateful to Dr. Kenichiro Nagai (School of Pharmacy, Kitasato University) for mass spectra measurements and to Dr. Hiroshi Kitazato (JAMSTEC) for coordination of the research cruise of the Iatá-Piúna. This work was supported by a Tohoku Ecosystem-Associated Marine Sciences research project of the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan.

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Affiliations

  1. Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Tokyo, Japan

    • Konami Takahashi
  2. Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Kanagawa, Japan

    • Konami Takahashi
    • , Yuriko Nagano
    • , Sakiko Orui Sakaguchi
    • , Kiyotaka Takishita
    • , Takao Yoshida
    •  & Katsunori Fujikura
  3. Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan

    • Konami Takahashi
    • , Kazunari Sakai
    • , Masato Iwatsuki
    • , Kenichi Nonaka
    •  & Satoshi Ōmura
  4. Graduate School of Infection Control Sciences, Kitasato University, Tokyo, Japan

    • Wataru Fukasawa
    • , Masato Iwatsuki
    •  & Kenichi Nonaka
  5. Department of Biological Sciences, University of Vale do Itajaí, CTTMar, R. Uruguai, 458-CEP, 88302-202, Itajaí, Brazil

    • Andre O. Lima
  6. Institute of Oceanography, University of São Paulo, 191. Praca do Oceanografico, São Paulo, 05508120, Brazil

    • Vivian H. Pellizari

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Conflict of interest

The authors declare that they have no conflict of interest.

Corresponding authors

Correspondence to Kenichi Nonaka or Satoshi Ōmura.

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DOI

https://doi.org/10.1038/s41429-018-0053-z