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Cell-adhesive gels made of sacran/collagen complexes

Polymer Journal volume 54, pages 581–589 (2022)Cite this article

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Abstract

Anionic polysaccharides (“sacran”) are expected to be useful as medical materials, such as cell cultivation scaffolds. In this study, polymer complex gels of sacran with cell-compatible proteins and collagen were prepared. Complex gels of sacran and collagen were observed using fluorescence and polarized microscopes, which indicated the complexation characteristics of sacran and collagen, as well as their partial orientation. Infrared spectroscopy revealed the gel formation of the polymer complexes. The addition of NaCl was observed to control the uniformity of the complex gels, which suggests that the main interaction in the complexation of anionic sacran with cationic functional groups of collagens is ionic. The swelling degree of the complex gel was significantly lower than that of the sacran-only gel, and the degree of cell adhesion of the complex gel was as high as that on tissue culture-treated polystyrene (positive control); this indicates that the introduction of collagen into sacran gels is effective for cell cultivation.

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Acknowledgements

This work was financially supported by the Shibuya Science and Sports Culture Foundation and partially supported by a Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Early-Career Scientists (21K14681) and the A-step (AS2915173U) of the Japan Science and Technology Agency (JST), Japan.

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Authors and Affiliations

  1. Graduate School of Advanced Science and Technology, Japan Advanced Institute of Science and Technology, Nomi, Ishikawa, Japan

    Kenji Takada, Asuka Komuro, Mohammad Asif Ali, Maninder Singh, Maiko Okajima, Kazuaki Matsumura & Tatsuo Kaneko

  2. Soft Matter Sciences and Engineering Laboratory, ESPCI Paris, PSL University, CNRS, Paris, France

    Mohammad Asif Ali

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  1. Kenji Takada
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  2. Asuka Komuro
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Correspondence to Tatsuo Kaneko.

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Takada, K., Komuro, A., Ali, M.A. et al. Cell-adhesive gels made of sacran/collagen complexes. Polym J 54, 581–589 (2022). https://doi.org/10.1038/s41428-021-00593-w

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