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Cell detachment from monolayer- and bilayer-type gold nanoparticle-containing collagen coatings by visible laser irradiation for cell sorting applications

Polymer Journal volume 53, pages 1485–1491 (2021)Cite this article

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Abstract

Cell sorting is important in many studies, from basic science, such as cell biology, to biomedical applications, such as cell engineering and regenerative medicine. We have developed a photoinduced in situ cell detachment system using gold nanoparticle (AuNP)-embedded collagen hydrogels as a spatiotemporal cell sorting system, which is an image-guided cell sorting system. Here, we prepared AuNP-containing collagen coatings as a photosensitive cell scaffold. Various cells cultured on the coating materials were detached by photoirradiation and subsequent medium injection. The monolayer type of AuNP/collagen coatings showed efficient cell detachment with cell damage and may be applied to remove undesired cells. For cell damage suppression, the bilayer-type AuNP/collagen coatings were designed and prepared. We demonstrated the sorting of visually changed transfected CHO-K1 cells and human-induced pluripotent stem cell-derived cardiomyocytes using our system, which is useful for genome editing and regenerative medicine, respectively.

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Acknowledgements

We are grateful to Yusei Takeyasu (Osaka Pref. Univ.) for his help in the preparation of coating substrates.

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Authors and Affiliations

  1. Department of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, Osaka, Japan

    Chie Kojima, Anri Kanetsuki & Yusuke Nakajima

  2. Optical Research Laboratory, Research & Development Division, Nikon Corporation, Yokohama, Kanagawa, Japan

    Takeshi Kawano, Kenji Takatsuka & Shuhei Tanaka

  3. Institute of Advanced Biomedical Engineering and Science, TWIns, Tokyo Women’s Medical University, Shinjuku-ku, Tokyo, Japan

    Yuji Haraguchi, Katsuhisa Matsuura & Tatsuya Shimizu

Authors
  1. Chie Kojima
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  2. Anri Kanetsuki
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  3. Yusuke Nakajima
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  4. Takeshi Kawano
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  5. Kenji Takatsuka
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  6. Shuhei Tanaka
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  7. Yuji Haraguchi
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  8. Katsuhisa Matsuura
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  9. Tatsuya Shimizu
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Corresponding author

Correspondence to Chie Kojima.

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Conflict of interest

KM and TS are inventors of a bioreactor system for culturing human iPS cells and differentiating human iPS cells into cardiac cells. Osaka Prefecture University and Tokyo Women’s Medical University received the research grant from Nikon Corporation.

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Kojima, C., Kanetsuki, A., Nakajima, Y. et al. Cell detachment from monolayer- and bilayer-type gold nanoparticle-containing collagen coatings by visible laser irradiation for cell sorting applications. Polym J 53, 1485–1491 (2021). https://doi.org/10.1038/s41428-021-00527-6

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  • DOI: https://doi.org/10.1038/s41428-021-00527-6

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