We demonstrated Japanese rice wine (sake) tasting using a fluorescent chemosensor array comprising polythiophene-based chemosensors functionalized with pyridinium boronic acid. The chemosensors bound to the components of sake (i.e., glucose and pyruvate) and showed changes in fluorescence as a result. To achieve on-site analysis, the chemosensor microarray was fabricated on a glass chip, for which an image pattern was rapidly recorded by using a CCD camera equipped with color filters. For high throughput and accurate analysis, the collected datasets were analyzed using pattern recognition techniques, and we discriminated between four nondiluted sakes based on the cross-reactive response of the polythiophene-based chemosensors. Moreover, the facile chemosensor sensor array succeeded in predicting unknown concentrations of glucose and pyruvate in diluted sake, which revealed its usability for drinks analysis.
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TM thanks JSPS KAKENHI (Grant Nos. JP20K21204 and JP21H01780) and JST CREST (Grant No. JPMJCR2011). The authors greatly thank Dr Yui Sasaki for providing helpful discussions. The authors also thank Satoshi Ito for polymer synthesis and Takashi Matsuki of Kyoto Electronics Manufacturing Co. Ltd. for supplying the sake samples with the HPLC and enzymatic analytical results.
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The authors declare no competing interests.
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Lyu, X., Matsumoto, A. & Minami, T. A polythiophene-based chemosensor array for Japanese rice wine (sake) tasting. Polym J 53, 1287–1291 (2021). https://doi.org/10.1038/s41428-021-00521-y