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Effect of microtubule immobilization by glutaraldehyde on kinesin-driven cargo transport


The glutaraldehyde fixation method for fixing tissues is attractive for its ease of use and straightforward surface chemistry. We investigated the effect of glutaraldehyde-induced microtubule immobilization on kinesin-driven cargo transport along microtubules and found that at low glutaraldehyde concentrations, the microtubule–kinesin interaction remains unperturbed. Such findings may facilitate the application of the glutaraldehyde fixation method for many in vitro studies aiming to build nanodevices powered by the microtubule–motor protein interaction.

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This work was financially supported by the Future AI and Robot Technology Research and Development Project from New Energy and Industrial Technology Development Organization (NEDO), Japan, Grant-in-Aid for Scientific Research on Innovative Areas “Molecular Engine” (JP18H05423) and Grant-in-Aid for Scientific Research (A) (JP18H03673) to AK from the Japan Society for the Promotion of Science (JSPS).

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Correspondence to Akira Kakugo.

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Nasrin, S.R., Kabir, A.M.R., Sada, K. et al. Effect of microtubule immobilization by glutaraldehyde on kinesin-driven cargo transport. Polym J 52, 655–660 (2020).

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