Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter to the Editor
  • Published:

A clinical study of preimplantation DNA methylation screening in assisted reproductive technology

Cell Research volume 33, pages 483–485 (2023)Cite this article

Subjects

Dear Editor,

Assisted reproductive technology (ART) has been used for more than 45 years, by which more than 9 million infants were born. One of the key factors to determine the success rate of the ART is embryo selection.1,2 Due to the existence of aneuploidy in 20%–30% of blastocysts, preimplantation genetic screening (PGS) has been widely used to increase live birth rate. However, the live birth rate remains around 50% even with the help of PGS.2 DNA methylation is known to play an important role during embryogenesis.3,4,5 A previous study showed that a large proportion of human embryos have abnormal DNA methylome, and indicated that preimplantation DNA methylation screening (PIMS) can analyze both copy number variation (CNV) and global DNA methylation level.5 However, whether DNA methylation patterns can affect the clinical outcome of ART has not been investigated in clinics. In this regard, we performed a clinical trial of PIMS (trial number: NCT03642574). We aimed to examine the relationship between embryo methylome and the clinical outcome of ART.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: The clinical outcomes of PIMS.

Data availability

The data that support the findings in this study are deposited in the Genome Sequence Archive (GSA) for human under the accession number HRA002940. Our data are available with a signed data use agreement; please contact liuj@big.ac.cn. The downstream reuse of the controlled access datasets is restricted to non-profitable usage.

References

  1. Gardner, D. K., Lane, M., Stevens, J., Schlenker, T. & Schoolcraft, W. B. Fertil. Steril. 73, 1155–1158 (2000).

    Article  CAS  PubMed  Google Scholar 

  2. Munné, S. et al. Fertil. Steril. 112, 1071–1079.e7 (2019).

    Article  PubMed  Google Scholar 

  3. Jiang, L. et al. Cell 153, 773–784 (2013).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Wang, L. et al. Cell 157, 979–991 (2014).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Li, G. et al. J. Genet. Genomics 44, 475–481 (2017).

    Article  PubMed  Google Scholar 

  6. Glick, I., Kadish, E. & Rottenstreich, M. Int. J. Womens Health 13, 751–759 (2021).

    Article  PubMed  PubMed Central  Google Scholar 

  7. Yan, J. et al. N. Engl. J. Med. 385, 2047–2058 (2021).

    Article  CAS  PubMed  Google Scholar 

  8. Fauque, P. et al. Clin. Epigenetics 12, 191 (2020).

    Article  PubMed  PubMed Central  Google Scholar 

  9. Vermeiden, J. P. & Bernardus, R. E. Fertil. Steril. 99, 642–651 (2013).

    Article  PubMed  Google Scholar 

  10. Cortessis, V. K. et al. J. Assist. Reprod. Genet. 35, 943–952 (2018).

    Article  PubMed  PubMed Central  Google Scholar 

  11. Court, F. et al. Genome Res. 24, 554–569 (2014).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Zink, F. et al. Nat. Genet. 50, 1542–1552 (2018).

    Article  CAS  PubMed  Google Scholar 

  13. Joshi, R. S. et al. Am. J. Hum. Genet. 99, 555–566 (2016).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Jiao, X. et al. Clin. Epigenetics 11, 120 (2019).

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

This work was supported by the grants from the National Key R&D Program of China (2022YFC2703200, 2021YFC2700600, 2021YFC2700500); Research Unit of Gametogenesis and Health of ART-Offspring, the Chinese Academy of Medical Sciences; Special Foundation of President of the Chinese Academy of Sciences; the National Natural Science Foundation of China (31871454 to J.L., 32121001to J.L.)

Author information

Author notes

  1. These authors contributed equally: Yuan Gao, Lizhi Yi, Jianhong Zhan, Lijuan Wang, Xuelong Yao.

Authors and Affiliations

  1. Center for Reproductive Medicine, Shandong University, Jinan, Shandong, China

    Yuan Gao, Lijuan Wang, Junhao Yan, Ming Gao, Yang Zou, Xuan Gao, Keliang Wu & Zi-Jiang Chen

  2. Shandong Key Laboratory of Reproductive Medicine, Shandong Provincial Clinical Research Center for Reproductive Health, Shandong Technology Innovation Center for Reproductive Health, Jinan, Shandong, China

    Yuan Gao, Lijuan Wang, Junhao Yan, Ming Gao, Yang Zou, Xuan Gao, Keliang Wu & Zi-Jiang Chen

  3. Key Laboratory of Reproductive Endocrinology of Ministry of Education, National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University, Jinan, Shandong, China

    Yuan Gao, Lijuan Wang, Junhao Yan, Ming Gao, Yang Zou, Xuan Gao, Keliang Wu & Zi-Jiang Chen

  4. CAS Key Laboratory of Genome Sciences and Information, Collaborative Innovation Center of Genetics and Development, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China

    Lizhi Yi, Jianhong Zhan, Sijing Jian, Lei Gao, Mamadboqirova Farangez & Jiang Liu

  5. China National Center for Bioinformation, Beijing, China

    Lizhi Yi, Jianhong Zhan, Sijing Jian, Lei Gao, Mamadboqirova Farangez & Jiang Liu

  6. Nvwa life technology, Guangzhou, Guangdong, China

    Lizhi Yi & Xuelong Yao

  7. Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai, China

    Zi-Jiang Chen

Authors
  1. Yuan Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lizhi Yi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jianhong Zhan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lijuan Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xuelong Yao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Junhao Yan
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Sijing Jian
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Lei Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Mamadboqirova Farangez
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Ming Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Yang Zou
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Xuan Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. Keliang Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  14. Jiang Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  15. Zi-Jiang Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

J.L., Z.C., and Y.G. conceived and designed the study. L.Y., J.Z., L.W., X.Y., J.Y., S.J., L.G., M.F., M.G., Y.Z., X.G., and K.W. conducted the experiments. L.Y. and J.Z. performed the bioinformatics analyses. J.L. and J.Z. wrote the manuscript.

Corresponding authors

Correspondence to Yuan Gao, Jiang Liu or Zi-Jiang Chen.

Ethics declarations

Competing interests

X.Y. and J.L. are shareholders of Nvwa life technology. The other authors declare no competing financial interests.

Supplementary information

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Gao, Y., Yi, L., Zhan, J. et al. A clinical study of preimplantation DNA methylation screening in assisted reproductive technology. Cell Res 33, 483–485 (2023). https://doi.org/10.1038/s41422-023-00809-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41422-023-00809-z

This article is cited by

Search

Advanced search

Quick links