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Structures of the endogenous peptide- and selective non-peptide agonist-bound SSTR2 signaling complexes

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Fig. 1: Cryo-EM structures of the SSTR2–Gi1 complexes.

Data availability

The atomic coordinates and the electron microscopy maps of SS-14- and L-054,264-bound SSTR2–Gi1 complexes have been deposited in the Protein Data Bank (PDB) under accession numbers 7WIC, 7WIG and Electron Microscopy Data Bank (EMDB) under accession codes EMD-32528, EMD-32529, respectively.

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Acknowledgements

The cryo-EM data were collected at the Cryo-Electron Microscopy Center, Zhejiang University. Protein purification was performed at the Protein Facilities, Zhejiang University School of Medicine. This project was supported by the National Natural Science Foundation of China (81922071 to Y.Z. and 32100959 to C.M.), Zhejiang Province Natural Science Fund for Excellent Young Scholars (LR19H31000 to Y.Z. and LR22C050002 to C.M.), the Ministry of Science and Technology (2019YFA050880 to Y.Z.), and the Key R&D Projects of Zhejiang Province (2021C03039 to Y.Z.).

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Y.Z. and C.M. conceived and supervised the whole project; C.M. purified the SSTR2–Gi1 complexes; D.-D.S. evaluated the sample by negative-stain EM; S.-Y.J. prepared the cryo-EM grids; J.G. and J.Q. collected the cryo-EM data; L.-N.C. performed cryo-EM map calculation and model building; L.-N.C. and Y.-J.D. generated the constructs of mutants; L.-N.C., W.-W.W., X.Y. and Q.L. performed the cellular functional assays; L.-N.C. and Y.-J.D. prepared the figures; Q.S., H.Z., Q.H. and B.Y. participated in data analysis. Q.L. edited the manuscript. C.M. and Y.Z. wrote the manuscript with inputs from all the authors.

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Correspondence to Qinglin Li or Chunyou Mao.

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Chen, LN., Wang, WW., Dong, YJ. et al. Structures of the endogenous peptide- and selective non-peptide agonist-bound SSTR2 signaling complexes. Cell Res 32, 785–788 (2022). https://doi.org/10.1038/s41422-022-00669-z

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