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Structures of human bile acid exporter ABCB11 reveal a transport mechanism facilitated by two tandem substrate-binding pockets

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Fig. 1: Structure determination and biochemical analysis of substrate-bound ABCB11.

Data availability

All relevant data are available from the authors and/or included in the manuscript or Supplementary information. Atomic coordinates and EM density maps of the human ABCB11 with two TCs (3.7 Å, PDB: 7DV5; EMDB: 30870) and ABCB11 with one TC (3.7 Å, PDB: 7E1A; EMDB: 30938) in this paper have been deposited in the Protein Data Bank and the Electron Microscopy Data Bank, respectively. The EM density map of ABCB11ΔN44 has been deposited in the Electron Microscopy Data Bank (EMDB: 32261).

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Acknowledgements

We thank Dr. Yongxiang Gao at the Center for Integrative Imaging, Hefei National Laboratory for Physical Sciences at the Microscale, University of Science and Technology of China during cryo-EM image acquisition. We thank Mr. Jishu Ren at the Isotope Laboratory, University of Science and Technology of China for the transport assays. We also thank Prof. Huaqiang Xu at Shanghai Institute of Materia Medica, Chinese Academy of Sciences for the assistance with paper revision. This work was supported by the Ministry of Science and Technology of China (2019YFA0508500), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDB37020202), and the Fundamental Research Funds for the Central Universities (YD20700002005).

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Contributions

Y.C. and C.Z.Z. conceived the project and designed the experiments. L.W., J.W., and C.G. expressed and purified human ABCB11. L.W. performed initial negative-staining and cryo-EM analyses and prepared all grids. L.W., W.T.H. and K.R. performed functional assays. L.W. and D.X. performed cryo-EM data collection. L.W. and L.S. performed ABCB11 structure determination and model refinement. W.-T.H., L.W., C.Z.Z. and Y.C. wrote the manuscript.

Corresponding authors

Correspondence to Wen-Tao Hou, Cong-Zhao Zhou or Yuxing Chen.

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The authors declare no competing interests.

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Wang, L., Hou, WT., Wang, J. et al. Structures of human bile acid exporter ABCB11 reveal a transport mechanism facilitated by two tandem substrate-binding pockets. Cell Res 32, 501–504 (2022). https://doi.org/10.1038/s41422-021-00611-9

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