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Cryo-EM structure of the AVP–vasopressin receptor 2–Gs signaling complex

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Fig. 1: Structure of the AVP–V2R–Gs complex.

Data availability

The structural restraints ad coordinates of the AVP-V2R-Gs complex have been deposited in the Protein Data Bank (PDB: 7KH0) and the Electron Microscopy Data Bank (EMDB: EMD-22872).

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Acknowledgements

We thank the Cryo-EM Facility Center of the Chinese University of Hong Kong, Shenzhen for providing technical support during EM image acquisition. Y.D. is supported by a grant from Science, Technology and Innovation Commission of Shenzhen Municipality (Project JCYJ20200109150019113), and in part by the Kobilka Institute of Innovative Drug Discovery and Presidential Fellowship at the Chinese University of Hong Kong, Shenzhen. C.Z. is supported by the grant R35GM128641 from the National Institutes of Health, the United States of America.

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Contributions

L.W. designed the expression constructs and purified the AVP–V2R–Gs complex. L.W. and S.C. prepared the samples for cryo-EM data collection. L.W. and J.X. performed screening of cryo-EM grids and data collection. J.X. processed cryo-EM data. L.W. built the model. D.S. and C.Z. refined the structure model. Z.L., Q.L. and S.C. assisted cryo-EM data collection and processing. H.L. assisted cloning. Y.D. and C.Z. supervised all studies, and C.Z. wrote the manuscript with Y.D., L.W. and J.X.

Corresponding authors

Correspondence to Yang Du or Cheng Zhang.

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The authors declare no competing interests.

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Wang, L., Xu, J., Cao, S. et al. Cryo-EM structure of the AVP–vasopressin receptor 2–Gs signaling complex. Cell Res (2021). https://doi.org/10.1038/s41422-021-00483-z

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