Abstract
Complement is known to play a role in alcoholic fatty liver disease (AFLD), but the underlying mechanisms are poorly understood, thereby constraining the development of a rational approach for therapeutic intervention in the complement system. C3 deficiency has been shown to impart protective effects against ethanol-induced hepatic steatosis and inflammation. Here we demonstrate a protection effect in wild-type mice by treatment with CR2-Crry, a specific inhibitor of C3 activation. The expression of glycine transfer (t) RNA-derived fragments (Gly-tRFs) is upregulated in ethanol-fed mice and inhibition of Gly-tRFs in vivo decreases chronic ethanol feeding-induced hepatosteatosis without affecting inflammation. The expression of Gly-tRF was downregulated in C3-deficient or CR2-Crry-treated mice, but not in C5-deficient mice; Gly-tRF expression was restored by the C3 activation products C3a or Asp (C3a-des-Arg) via the regulation of CYP2E1. Transcriptome profiling of hepatic tissues showed that Gly-tRF inhibitors upregulate the expression of sirtuin1 (Sirt1) and subsequently affect downstream lipogenesis and β-oxidation pathways. Mechanistically, Gly-tRF interacts with AGO3 to downregulate Sirt1 expression via sequence complementarity in the 3′ UTR. Notably, the expression levels of C3d, CYP2E1 and Gly-tRF are upregulated, whereas Sirt1 is decreased in AFLD patients compared to healthy controls. Collectively, our findings suggest that C3 activation products contribute to hepatosteatosis by regulating the expression of Gly-tRF. Complement inhibition at the C3 activation step and treatment with Gly-tRF inhibitors may be potential and precise therapeutic approaches for AFLD.
Similar content being viewed by others
Introduction
Alcoholic liver disease is a leading cause of morbidity and mortality worldwide. Alcoholic fatty liver disease (AFLD) is a consequence of excessive alcohol consumption. When patients continue to drink, AFLD progresses to more severe forms of liver injury such as steatohepatitis, fibrosis, cirrhosis, and hepatocellular carcinoma.1 Excessive ethanol intake is estimated to be the cause of 4.5% of these diseases and 3.8% of all deaths around the world.2,3 AFLD is characterized by a number of symptoms, such as steatosis and inflammation, which contribute to liver injury. While AFLD has been extensively studied, current understanding of the pathogenesis of AFLD is still limited.
Several lines of evidence have shown that different mediators, such as the complement system, reactive oxygen species (ROS), neutrophils, macrophages and lipopolysaccharide (LPS), play critical roles in the development of AFLD. Complement activation is involved in ethanol-induced liver injury,4,5 and mice lacking complement component 3 (C3) are protected from ethanol-induced steatosis.4 In contrast, complement component 5 (C5) deficiency does not affect steatosis, although it imparts a protective effect on ethanol-induced inflammatory responses.4 Upon activation, C3 is cleaved into C3a and C3b, and C3b further promotes the activation/cleavage of C5 to C5a and C5b.6 C3a and C5a have been correlated with increased expression of cytokines and chemokines, which facilitate the progression of alcohol-induced liver inflammation. However, the underlying mechanisms of how C3 contributes to ethanol-induced steatosis are not well understood. Furthermore, there are no effective drugs for the therapy of AFLD. Liver transplantation is an accepted and successful therapy for severe AFLD patients, and liver resection is one of the radical treatments for hepatocellular carcinoma patients with an AFLD background. Liver ischemia-reperfusion injury (IRI) following surgery is a key contributor to liver dysfunction and failure, and there is currently no therapeutic regimen for preventing or minimizing hepatic IRI after transplantation or resection.
Complement receptor 2 (CR2)-Crry is a site-targeted complement inhibitor that blocks all complement pathways at the C3 activation step.7 The complement inhibitory moiety, Crry, is targeted via the CR2 fusion partner that binds C3d, a complement activation product deposited at sites of complement activation. We have previously shown that CR2-Crry can provide protection against hepatic IRI in some, but not all settings.8,9 Effective complement inhibtitory function of CR2-Crry is dependent on its localized targeting,7 and its effect on liver IRI in an AFLD background has not been previously investigated.
In the past two decades, various noncoding RNAs (ncRNAs) have been shown to play an important role in regulating gene expression. The development of small RNA high-throughput sequencing technologies has led to the discovery of diverse classes of ncRNAs. In addition to microRNAs (miRNAs), small RNAs can be processed from novel RNA sources such as transfer (t)RNA, small nucleolar (sno)RNA, and ribosomal (r)RNA.10,11,12,13 In recent years, small RNAs derived from tRNAs have been investigated in various research fields.14,15,16,17,18,19 Several lines of evidence indicate that tRNA-derived fragments (tRFs) are functional molecules involved in various biological processes,14,20,21,22 including the regulation of gene expression, and it has been shown that stress-induced tRFs can inhibit protein synthesis.23 Gebetsberger et al.24 showed that tRFs regulate translation during stress by competing with mRNA for ribosome binding. In addition, tRFs generated by specific cleavage of mature tRNAs are induced by a variety of stressors such as oxidative stress, heat/cold shock, and ultraviolet irradiation,25,26,27,28 and endonucleolytic cleavage of tRNAs is a widely conserved response to oxidative stress in eukaryotes.29 Moreover, angiogenin (ANG) is a stress-activated ribonuclease that is involved in tRF biogenesis.23,27,28 Ethanol consumption promotes the production of ROS, mainly hydrogen peroxide (H2O2) and superoxide anion (O2−), in the liver,30 and oxidative stress plays a prominent role in the pathogenesis of AFLD.31,32,33 Although the function of tRFs has been extensively studied, the role and molecular mechanisms of tRFs in AFLD are unknown.
SIRT1, an NAD+-dependent deacetylase, is a central molecule in hepatic lipid metabolism and AFLD pathogenesis.34,35,36,37 Deletion of the hepatic gene Sirt1 results in stimulated lipogenesis and impaired β-oxidation,38 and SIRT1 triggers lipid metabolism by regulating numerous genes such as Srebp-1c,34,39 Lipin1,38 Ppara,36 and Cpt1a,38 which play major roles in AFLD development. Although a role for SIRT1 signaling in AFLD development is established, the molecular mechanisms associated with its disruption in response to ethanol challenge remain elusive.
In this study, we explored the underlying mechanisms whereby C3 contributes to ethanol-induced hepatic steatosis, and we assessed the therapeutic effect of CR2-Crry in ethanol-induced liver steatosis and inflammation in mice. Furthermore, we analyzed the roles of Gly-tRF and their link to complement activation in an AFLD mouse model, and evaluated potential therapeutic strategies using Gly-tRF antisense inhibitors.
Results
Therapeutic effect of targeted complement inhibitor CR2-Crry or C3 deficiency on ethanol-induced hepatic steatosis and liver injury
Studies have shown that C3 activation is involved in the development of AFLD.4,5 CR2-Crry is a site-targeted complement inhibitor of C3 activation,7,8,9 and humanized versions of site-targeted C3 inhibitors are in clinical development. We therefore investigated the therapeutic effect of CR2-Crry on ethanol-induced hepatic steatosis and inflammation. Mice were given ethanol via an intragastric gavage or by short-term binge feeding. Serum C3a levels were elevated after ethanol feeding in wild-type (WT) mice, but not in C3−/− mice (Fig. 1a). C3a levels were decreased in ethanol-fed CR2-Crry-treated mice compared to untreated mice (Fig. 1a; Supplementary information, Fig. S1a). C3d accumulation also decreased in C3-deficient and CR2-Crry-treated mice compared to untreated mice after ethanol feeding (Fig. 1b; Supplementary information, Fig. S1b). Hematoxylin and eosin (H&E) staining showed that hepatic steatosis was significantly reduced in ethanol-fed CR2-Crry-treated mice compared to untreated mice, and fat deposits were almost undetectable in C3−/− mice (Fig. 1b, c). Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were increased in the ethanol-fed WT mice compared to the pair-fed mice, but were significantly decreased in ethanol-fed CR2-Crry-treated WT mice as well as C3−/− mice compared to untreated mice (Fig. 1d; Supplementary information, Fig. S1c). Inflammatory cytokines, such as tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6) were upregulated in response to ethanol exposure, but C3 deficiency or CR2-Crry treatment imparted protective effects against ethanol-induced inflammation (Fig. 1e; Supplementary information, Fig. S1d, e). Furthermore, the expression levels of Ly6G, IL-1β, Cxc14 and ICAM1 were also elevated in ethanol-fed mice, but such elevation was abolished in C3−/− or CR2-Crry-treated mice (Fig. 1f). Several lines of evidence indicate that ethanol metabolism impairs hepatocyte function, leading to apoptosis in AFLD.40,41,42 We therefore evaluated the effect of CR2-Crry on apoptosis. Hepatocyte apoptosis was markedly increased in ethanol-fed WT mice, but C3 deficiency or CR2-Crry treatment abolished the effect (Supplementary information, Fig. S2a). Apoptosis-related genes were further analyzed by western blot. We found that the expression levels of caspase-3, caspase-8, caspase-9 and Bax were elevated in the ethanol-fed mice, but this effect was reversed by C3 deficiency or CR2-Crry treatment. In contrast, Bcl2 expression was diminished in ethanol-fed mice, but was enhanced in C3-deficient or CR2-Crry-treated mice (Supplementary information, Fig. S2b). We next examined the expression of STAT3, p-STAT3, Akt and p-Akt in liver homogenates by western blot. The ratio of p-Akt/Akt expression was decreased in ethanol-fed mice, but C3 deficiency or CR2-Crry treatment increased the ratio compared to untreated mice. In addition, the ratio of p-STAT3/STAT3 expression was also increased by CR2-Crry treatment (Supplementary information, Fig. S2c).
CR2-Crry treatment protects against IRI in AFLD mice
Liver IRI is an unavoidable outcome in surgical procedures, and IRI is a major factor contributing to postsurgical liver dysfunction and liver failure.8,43,44 Complement activation can increase the susceptibility of steatotic livers to IRI.44 He et al.8 reported that CR2-Crry treatment protects against IRI in a murine ligation model, but not against liver IRI when ischemia is induced in the context of resection. It is therefore important to determine how targeted inhibition of C3 impacts liver IRI in an AFLD model. Mice were subjected to 30 min of total warm hepatic ischemia followed by reperfusion, and were treated with PBS or CR2-Crry 30 min before ischemia. After 6 h of reperfusion, we found that ALT and AST levels were elevated in ethanol-fed mice compared to pair-fed mice, but the elevation was eliminated by CR2-Crry treatment. Furthermore, ethanol-induced steatosis increased the susceptibility of livers to warm hepatic IRI (Fig. 2a, b). C3 was activated in the ethanol-fed mice, but C3 activation was inhibited by CR2-Crry treatment (Fig. 2c). In addition, TNF-α and IL-6 levels were increased in the ethanol-fed mice, and CR2-Crry treatment significantly reduced the levels of both cytokines (Fig. 2d, e). Thus, CR2-Crry treatment protects against liver IRI.
Complement C3 regulates the expression of CYP2E1
Oxidative stress is a predominant factor that contributes to the pathogenesis of AFLD.31,32 We therefore investigated the effect of C3 deficiency or CR2-Crry treatment on the expression of CYP2E1, a member of the cytochrome P450 mixed function oxidase system. We found that the CYP2E1 expression and activity were increased in ethanol-fed WT mice, but this effect was abolished in C3−/− or CR2-Crry-treated mice (Fig. 3a, b). Furthermore, glutathione (GSH) and superoxide dismutase (SOD) levels were diminished in ethanol-fed mice, but their levels were recovered in C3−/− or CR2-Crry-treated mice. In contrast, malondialdehyde (MDA) level was elevated in ethanol-fed mice, but C3 deficiency or CR2-Crry treatment reduced the MDA level (Fig. 3c, d). The decreased expression of CYP2E1 in C3−/− mice was restored by treatment with the peptide C3a or its degraded form, C3a-des-Arg (also known as Asp), again indicating a causative link with C3 activation (Fig. 3e). Although not without controversy, C5aR2 is the only identified receptor for Asp. Notably, the expression of CYP2E1 decreased upon C5aR2 knockdown, and Asp treatment failed to restore the expression of CYP2E1 (Fig. 3f). Previous studies have shown that C5aR2 binds to β-arrestin2, thereby mediating other signaling pathways involved in transcriptional regulation of CYP2E1.45,46 Co-immuoprecipitation showed that β-arrestin2 binds to C5aR2 in liver tissue of ethanol-fed mice, which was reduced following the decrease of C3 stimulation in ethanol-fed C3−/− mice (Fig. 3g). Furthermore, β-arrestin2 knockdown by siRNA resulted in the downregulation of CYP2E1 expression (Fig. 3h). In addition, adeno-associated virus 9 (AAV9)-shRNAs or CMZ, an inhibitor of CYP2E1, was used to downregulate Cyp2e1 (Supplementary information, Fig. S3a, b), and the effect on liver steatosis was evaluated. Cyp2e1-shRNAs or CMZ treatment lowered liver triglyceride levels and micro- and macro-vesicular fat accumulation in ethanol-fed mice (Fig. 3i, j).
C3 but not C5 regulates Gly-tRF expression contributing to steatosis in AFLD mice
Oxidative stress plays an important role in the development of AFLD, and ROS levels in the liver increase following alcohol consumption. tRNA cleavage is reportedly conserved in response to oxidative stress,29 and we hypothesized that a tRNA-derived fragment is a contributor to the development of AFLD. We performed small RNA high-throughput sequencing of hepatic tissues from short-term ethanol-fed mice followed by bioinformatics analysis. Small RNAs, but not microRNAs, can be generated from novel RNA sources such as tRNAs and rRNAs. We found that Gly-tRFs were upregulated in the liver of the ethanol-fed mice compared to the pair-fed mice (Fig. 4a). Sequence alignment analysis suggests that highly expressed Gly-tRFs (29–34 nt) were cleaved from the 5′ end of the tRNA precursors at the anticodon loop (Fig. 4a). Gly-tRF levels were verified by quantitative reverse transcription PCR (qRT-PCR), and as shown in Fig. 4b, short-term ethanol feeding increased the hepatic expression of Gly-tRF. Notably, the hepatic expression of Gly-tRFs was significantly upregulated in chronic ethanol-fed mice compared to pair-fed mice. To investigate whether Gly-tRF expression is induced by ethanol, AML12 liver cells were cultured in the presence of ethanol. Gly-tRF expression was increased by treatment with ethanol (Fig. 4c). We also evaluated the effect of H2O2 on Gly-tRF expression in AML12 cells, and found that H2O2 induced a dose-dependent increase in Gly-tRF levels (Supplementary information, Fig. S3c). Several studies have indicated that ANG induced by stress is involved in the biogenesis of tRFs.23,25 We used siRNA to knock down Ang, and its attenuation was validated by qRT-PCR and western blot analyses (Supplementary information, Fig. S3d). ANG downregulation decreased Gly-tRF expression after treatment with ethanol or H2O2, as determined by qRT-PCR (Fig. 4d; Supplementary information, Fig. S3e).
To investigate the role of Gly-tRF in AFLD, we used anti-sense inhibitors to block Gly-tRF expression in vivo in the AFLD mouse model. Compared to the controls, serum ALT and AST levels were significantly reduced in the Gly-tRF inhibitor treated mice (Fig. 4e). Oil Red and H&E staining showed that treatment with Gly-tRF inhibitors significantly decreased ethanol-induced micro- and macro-vesicular hepatic steatosis (Fig. 4f, g). However, the proinflammatory cytokines TNF-α and IL-6 were not affected by Gly-tRF inhibition (Fig. 4h). As described above, complement C3 regulates the expression of CYP2E1 in AFLD mice. Thus, we analyzed the expression of Gly-tRF in ethanol-fed C3−/− mice or in CR2-Crry-treated mice. Gly-tRF expression was significantly decreased in C3−/− mice or CR2-Crry-treated mice compared to untreated WT mice (Supplementary information, Fig. S3f). Furthermore, the expression of Gly-tRF in C3−/− mice was restored by treatment with peptide C3a or Asp, indicating a causative link (Fig. 4i). Knockdown of C5aR2 by AAV9-shRNA led to a decrease in the expression of Gly-tRF in AFLD mice, which was not restored by Asp (Fig. 4j). Furthermore, downregulation of CYP2E1 by shRNAs or CMZ decreased the hepatic expression of Gly-tRF in AFLD mice (Fig. 4k). Interestingly, C5 deficiency had little effect on the expression of Gly-tRF and liver steatosis in ethanol-fed mice (Fig. 4l), indicating that complement activation products downstream of C5 cleavage/activation are not involved in these pathogenic mechanisms.
Gly-tRF is involved in regulation of lipid metabolism pathway in AFLD mice
We next investigated the molecular mechanism associated with Gly-tRFs in the AFLD mouse model. Hepatic tissues from mice treated with Gly-tRF inhibitors were subjected to transcriptome sequencing, and Gly-tRF was shown to be associated with lipid metabolism in AFLD mice (Supplementary information, Fig. S4a). Genes related to fatty acid synthesis were downregulated, whereas β-oxidation-associated genes were upregulated by treatment with Gly-tRF antisense inhibitors (Supplementary information, Table S2). The results of qRT-PCR and western blot showed that hepatic expression of fatty acid synthesis related genes such as Srebp-1c, Fasn, Lipin1 and Acc was downregulated by Gly-tRF inhibitors (Fig. 5a; Supplementary information, Fig. S4b). In contrast, the expression of the β-oxidation-related genes Pparα and Cpt1a was upregulated (Fig. 5a; Supplementary information, Fig. S4c). In addition, the expression of Cyp2e1 and Ang was decreased by Gly-tRF inhibitor treatment (Supplementary information, Fig. S4d). Interestingly, transcriptome profiling indicated that Gly-tRF inhibitors upregulate the in vivo hepatic expression of Sirt1 (Supplementary information, Table S2). Previous studies have indicated that ethanol-induced attenuation of hepatic SIRT1 plays an important role in the pathogenesis of AFLD, and that stimulation of SIRT1 expression protected against the development of AFLD.34,36,47,48 We validated the hepatic expression of Sirt1 by qRT-PCR and western blot analyses, which showed that treatment with Gly-tRF inhibitors increased Sirt1 expression in hepatic tissues (Fig. 5b). Notably, the overexpression of Sirt1 via AAV9-Sirt1 alleviated liver steatosis in the AFLD mice (Fig. 5c; Supplementary information, Fig. S5a, b). Although the transcriptional level of C3 was not affected by Sirt1 overexpression, the activation level of C3 was decreased (Supplementary information, Fig. S5c). The expression of CYP2E1, ANG and Gly-tRF was downregulated by the overexpression of Sirt1 (Supplementary information, Fig. S5d, e). Srebp1 expression was downregulated by the overexpression of Sirt1, whereas the expression of Cpt1a was upregulated (Supplementary information, Fig. S5f), indicating that SIRT1 is a critical regulator of lipid metabolism pathways. We further used SIRT1 liver-specific knockout (LKO) mice to study the effect of Gly-tRF inhibitors. Treatment with Gly-tRF inhibitors downregulated the expression of Srebp1 and upregulated the expression of Ppara in ethanol-fed Sirt1fl/fl mice, but failed to further downregulate the expression of Srebp1 or to upregulate Ppara expression in ethanol-fed SIRT1 LKO mice (Fig. 5d). These results indicate that Gly-tRF regulates lipid metabolism by targeting Sirt1 in AFLD mice. Because C3 regulates the expression of Gly-tRF, we investigated whether C3 activation affects the expression of SIRT1 via mediating the expression of Gly-tRF. We found that SIRT1 expression was restored in C3-deficient or CR2-Crry-treated mice compared to untreated ethanol-fed mice (Fig. 5e). In addition, the protective effects of C3 deficiency was reversed by the knockdown of Sirt1 in the AFLD mice (Fig. 5f). These results indicate that Gly-tRF regulates lipid metabolism by targeting Sirt1 in AFLD mice.
To further explore the molecular mechanism of Gly-tRF regulation of Sirt1 expression, AML12 cells were transfected with Gly-tRF mimics, inhibitors or corresponding controls, and after 48 h the cells were analyzed by qRT-PCR and western blotting. Sirt1 expression was decreased in the presence of Gly-tRF mimics, but was upregulated by Gly-tRF inhibitors in AML12 cells (Fig. 5g; Supplementary information, Fig. S6a). We then predicted the binding sites of Gly-tRF in the Sirt1 3′ UTR using online software (Fig. 5h). The Sirt1 3′ UTR sequence was cloned into the pMir-reporter vector, and the construct was co-transfected with Gly-tRF mimics, inhibitors or corresponding controls into AML12 cells, respectively. After 48 h, cells were collected for luciferase assays. The results showed that luciferase expression decreased after treatment with Gly-tRF mimics, but increased with Gly-tRF inhibitors (Fig. 5i). Next, a mutation was introduced into the predicted binding sites (Fig. 5h), and a mutant-containing plasmid was co-transfected with Gly-tRF mimics, inhibitors or corresponding controls, respectively. The binding site mutation inhibited downregulation of luciferase expression by Gly-tRF or upregulation by Gly-tRF inhibitors (Supplementary information, Fig. S6b). In addition, when Sirt1 was downregulated by AAV9-shRNAs, Gly-tRF inhibitor did not further ameliorate liver steatosis in mice (Supplementary information, Fig. S6c, d). Studies have shown that AGO family proteins associate with tRFs that are involved in regulation of gene expression.18,49,50,51 Therefore, we used siRNAs to knock down Ago1, Ago2, Ago3, or Ago4 (Supplementary information, Fig. S6e), and revealed that luciferase expression was not affected by the silencing of Ago1, Ago2 and Ago4. However, upon Ago3 knockdown, Gly-tRF no longer inhibited the expression of luciferase (Fig. 5j). RIP-qPCR analysis indicated that AGO3, but not AGO2, significantly enriched Gly-tRFs (Fig. 5k). These results suggest that AGO3 mediates Gly-tRF regulation of gene expression.
The expression of C3d, CYP2E1, Gly-tRF and Sirt1 in AFLD patients
Although the therapeutic effects of CR2-Crry or Gly-tRF inhibitors in ethanol-fed mice indicate an underlying mechanism for the development of AFLD, whether these findings point to a potential strategy for treating AFLD requirs validation in human specimens. We therefore investigated the expression of C3d, CYP2E1, Gly-tRF and Sirt1 in the liver tissues of AFLD patients and healthy controls. Fat accumulation and liver triglyceride level increased in AFLD patients compared to healthy contols (Fig. 6a), and C3d accumulated in AFLD patients relative to controls (Fig. 6b). Notably, the levels of CYP2E1 and Gly-tRF were upregulated in AFLD patients compared to healthy controls (Fig. 6c, d). We further examined the expression of Sirt1 and showed that Sirt1 expression significantly decreased in liver tissues of AFLD patients compared to healthy controls (Fig. 6e). These results are consistent with our animal studies, indicating a potential clinical approach.
Taken together, the data elucidate the molecular mechanisms whereby C3 regulates liver steatosis and inflammation. Both C3a and Asp promote the expression of Gly-tRFs via mediating the expression of CYP2E1, which contributes to the development of liver steatosis by regulating Sirt1 expression (Fig. 6f).
Discussion
In this study, we investigated the related mechanisms of how complement contributes to AFLD and explored the therapeutic potential of a site-targeted complement inhibitor. We found that C3 activation is involved in regulation of both hepatosteatosis and inflammatory responses and contributes to liver IRI in AFLD mice. CR2-Crry treatment significantly reduced inflammation and hepatic steatosis in AFLD mice, in addition to alleviating apoptosis and liver IRI. Our data indicate that C3 activation products regulate the expression of CYP2E1 via the signaling of β-arrestin2. We show that expression of Gly-tRFs is upregulated in both ethanol-fed mice and AFLD patients. Inhibition of Gly-tRFs in vivo resulted in a reduction in hepatic steatosis, but not in inflammatory response in ethanol-fed mice. The expression of Gly-tRF was dowregulated in C3−/− mice or CR2-Crry-treated WT mice, which was restored by the C3 activation products C3a or Asp, indicating a novel mechanism by which C3 contributes to the development of hepatosteatosis. Futhermore, Gly-tRFs interact with AGO3 to downregulated Sirt1 expression via a complementary sequence in the 3′ UTR. As a result, fatty acid synthesis and lipid oxidation pathways are altered, leading to liver steatosis and injury.
Currently, there are few effective drugs approved for the treatment of AFLD. Previous studies have shown that C3 and C5 play different roles in the progression of AFLD.4 C3 deficiency protects against ethanol-induced liver injury and steatosis. In contrast, C5−/− mice still exhibit hepatosteatosis, but have lower inflammatory cytokines levels in the AFLD mouse model.4 In this study, we assessed the therapeutic effect of the site-targeted complement inhibitor, CR2-Crry, on AFLD in mouse models. CR2-Crry inhibits C3 convertase and thus suppresses C3 cleavage and activation, leading to a reduction in both inflammatory cytokine levels and hepatic steatosis after ethanol feeding. These results suggest that C3 inhibitors, in particular a humanized form of CR2-Crry, may provide an effective treatment for AFLD patients with both inflammatory response syndrome and hepatic steatosis. In addition, patients with advanced forms of AFLD usually require surgical intervention to improve survival. IRI is an inevitable consequence of surgical procedures, and complement activation plays a key role in hepatic IRI.8,9 Our results indicate that targeted C3 inhibition may provide a therapeutic strategy to protect against hepatic IRI for severe AFLD patients.
Oxidative stress is a typical feature of ethanol-induced AFLD, with ethanol degradation catalyzed by cytochrome P450 enzymes, including CYP2E1, as part of a major pathway. A previous study reported that hepatic steatosis is elevated in CYP2E1 knock-in mice after ethanol feeding.52 Pritchard et al.4 reported that ethanol feeding increases the expression of CYP2E1 in both WT and C3−/− mice compared to pair-fed mice. In the present study, however, CYP2E1 expression was reduced in ethanol-fed C3−/− or CR2-Crry-treated WT mice compared to ethanol-fed WT mice. Furthermore, the expression of CYP2E1 in C3−/− mice was restored by the treatment with C3a or Asp. Currently, C5aR2 is the only identified receptor for Asp,53,54 and C5aR2 plays a critical role in triglyceride synthesis.55 Knockdown of C5aR2 by AAV9-shRNAs reduced the expression of CYP2E1, which was not restored by Asp, indicating that C5aR2 is a key regulator of the development of AFLD. Previous studies have shown that C5aR2 binds to β-arrestin2,45 which acts as scaffolds for the β-catenin or JNK activation,46 and the β-catenin or JNK pathway is involved in transcriptional regulation of CYP2E1.56,57 Our studies indicate that Asp/C5aR2 regulates the expression of CYP2E1 through the β-arrestin2 signaling pathway.
The contribution of C3 to hepatosteatosis has been previously investigated. However, the mechanisms by which complement is involved in ethanol-induced steatosis are poorly understood. Small RNA high-throughput sequencing of hepatic tissues indicated that Gly-tRFs are upregulated in ethanol-fed mice compared to pair-fed mice. Previous studies have indicated that tRFs play critical roles in cellular processes and carcinogenesis.14,16 However, a role for Gly-tRFs in AFLD has not been previously described. In this study, Gly-tRF antisense inhibitor treatment significantly reduced hepatic steatosis in AFLD mice, but did not affect the inflammatory response. These results suggest that Gly-tRF was associated with the development of liver steatosis. In addition, C3 deficiency or CR2-Crry treatment decreased the expression of Gly-tRF. Furthermore, the expression of Gly-tRF in C3−/− mice was restored by administration of mice with the peptides C3a or Asp. Notably, C5 deficiency had little effect on the development of liver steatosis and the expression of Gly-tRF in AFLD mice. Several lines of evidence have shown that C3 activation contributes to both liver steatosis and inflammatory response, however, C5-deficient AFLD mice still exhibit hepatosteatosis but reduced inflammatory response.4 These studies further support that Gly-tRF is involved in regulation of liver steatosis but not inflammatory response. Following the generation of C3a, a serum carboxypeptidase rapidly cleaves its C-terminal Arg residue to form a more stable Asp. We found that C3a and Asp function similarly to regulate the expression of Gly-tRF. Notably, downregulation of CYP2E1 by shRNAs or CMZ decreased the expression of Gly-tRF in ethanol-fed mice. Taken together, C3a/Asp is a driving factor to regulate the expression of Gly-tRF through the CYP2E1 pathway. Chronic alcohol intake leads to AFLD, which encompasses a broad spectrum of diseases, including simple steatosis, steatohepatitis, fibrosis, and cirrhosis. More than 90% of heavy drinkers develop AFLD. Interventional therapy is conducted in this critical stage of liver steatosis, which may protect against the development of severe forms of AFLD. Together, these results indicate that inhibitors of Gly-tRF may be used for a potential precision therapy for patients with hepatosteatosis.
Our transcriptome sequencing data revealed that Sirt1 expression increases upon Gly-tRF antisense inhibitor treatment. Our results indicate that Gly-tRFs regulate Sirt1 expression in hepatic tissue of mice, which was further confirmed in vitro using AML12 cells. Studies have shown that SIRT1 can regulate diverse lipid metabolism pathways in the liver,36 including lipogenesis and fatty acid β-oxidation. Furthermore, hepatic SIRT1 signaling pathways play a predominant role in AFLD development. Hepatic deletion of Sirt1 facilitates the development of hepatic steatosis in response to ethanol feeding in mice, mainly by altering the function of LIPIN1, a transcriptional regulator of lipid metabolism.38 In addition, SIRT1 is a key regulator of Pparα signaling, which is the major regulator stimulating hepatic mitochondrial β-oxidation of fatty acids. Our transcriptome profiling data showed that genes related to fatty acid synthesis such as Srebp-1c, Lipin1, and Fasn were downregulated, whereas the Pparα and Cpt1a genes that are associated with fatty acid β-oxidation were upregulated by Gly-tRF inhibitors. Furthermore, the results of binding site mutations demonstrate that Gly-tRFs downregulate Sirt1 expression via 3′ UTR sequence complementarity. These results indicate that regulation of the SIRT1 pathways by Gly-tRFs represents a molecular mechanism for the development of ethanol-induced hepatic steatosis. These findings reveal a novel mechanism for regulating the expression of Sirt1, and indicate the Gly-tRF inhibition is a potential strategy for the restoration of ethanol-mediated impairment of SIRT1 in AFLD.
Several lines of evidence indicate that the complement system is activated in AFLD patients. Wlazlo et al.58 reported that activated C3 is associated with AFLD, and the immunoreactive intensity of C1q, C3, and C5 in patients with alcoholic hepatitis is significantly higher than that seen in normal controls.59 In addition, previous studies have shown that hepatic CYP2E1 can be induced by ethanol consumption and that CYP2E1 levels are higher in patients with AFLD.60,61,62 Interestingly, serum SIRT1 was downregulated in AFLD patients compared with healthy controls.63 Moreover, the mRNA level of Sirt1 was also lower in an AFLD group compared to a control group.63 Our study shows that C3d, CYP2E1 and Gly-tRF are upregulated, but that Sirt1 is downregulated, in AFLD patients compared to healty controls. These results are consistent with previous preclinical studies, indicating a potential therapeutic strategy for AFLD. Previous studies indicated that tRFs with different lengths processed from the same tRNA have different functions.64,65 In this study, we focused on the tRF with a length of 29–34 nt, which is fundamentally distinct from tRFs of ~22 nt in length.64 In addition, recent studies showed that RNA modifications are associated with the function of tRFs in sperm or stem cells.19,20 RNA modification of tRF in somatic cells is incompletely understood, and is an area for future study.
In summary, our results demonstrate that complement C3 inhibition mitigates the severity of early stage AFLD. We found that the C3 activation products C3a and Asp play key roles in the development of hepatic steatosis by regulating the expression of Gly-tRF via CYP2E1. Our study reveals a novel mechanism by which Gly-tRFs promote lipogenesis and inhibit fatty acid β-oxidation through regulating the SIRT1 signaling pathway. These findings establish a working model encompassing the complement-mediated regulation of Gly-tRF expression in AFLD, and reveal promising therapeutic targets for the management of the disease. Notably, humanized versions of complement inhibitors may potentially be employed in the treatment of AFLD, and inhibitors of Gly-tRF represent a potential precision therapy strategy for the liver steatosis of AFLD.
Materials and Methods
Mice and human specimens
C3−/− mice on C57BL/6 background (Stock# 003641), C57BL/6 WT, C5−/− (B10.D2-Hc0H2dH2-T18c/0SnJ) and their haplotype (B10.D2-Hc1H2dH2-T18c/nSnJ), SIRT1flox/flox (Stock# 029603) were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). Alb-cre mice were kindly provided by the Center of Shanghai Model Organisms. All liver tissue samples were collected from clinically defined AFLD patients and healthy controls as part of an approved institutional review board (IRB). Healthy liver tissue was obained from liver donation or benign liver diseases such as liver hemangioma. All animal experiments were approved by the Animal Care and Use Committee of Guangxi Medical University, Guangxi, China.
Ethanol feeding protocols
Eight- to ten-week-old female C57BL/6 WT, C3−/− mice and littermate WT mice were separately subjected to gavage, short-term binge feeding or chronic feeding. Eight- to ten-week-old female C5−/− and their haplotype mice were subjected to short-term binge feeding. The ethanol feeding protocols were modified from a previously described approach: 1) Ethanol gavage4: mice were gavaged with a single dose of ethanol (6 g/kg body weight, 31.5% ethanol), and PBS or CR2-Crry (dose: 0.25 mg) was injected intraperitoneally (i.p.) 30 min before gavage. 2) Short-term binge feeding66: mice were fed a control Lieber-DeCarli diet for five days to acclimatize them to a liquid diet, then allowed free access to the Lieber-DeCarli diet containing 5% (v/v) ethanol for 10 days; control groups were pair-fed with an isocaloric control diet. The mice were gavaged with a single dose of ethanol (6 g/kg body weight) or isocaloric dextrin-maltose on the 16th day and euthanized 9 h later. PBS or CR2-Crry (dose: 0.25 mg) was injected i.p. every other day for the last six days, and an additional injection was given 30 min before gavage. This protocol for chronic-plus-single-binge ethanol feeding synergistically induced both steatosis and inflammation, which mimics acute-on-chronic alcoholic liver injury in patients.66 We used this model to study the effect of complement inhibitor CR2-Crry on both steatosis and inflammation of ALD. 3) Chronic feeding4,67: the mice were randomly assigned to either pair-fed or ethanol-fed groups. The mice were allowed free access to the control liquid diet for three days, then the ethanol-fed group was allowed free access to the increasing concentrations of ethanol in a complete liquid diet. Control mice were given pair-fed diets, and the ethanol-fed mice were given ethanol at concentrations as follows: 1% ethanol for two days followed by 2% for two days, 4% for seven days, and 5% for three weeks. Mean body weights were similar in all groups at the end of the experiments. The chronic feeding model can mimic the pathogenesis of alcoholic fatty liver, but only induces little inflammation response. We used this model to investigate the role of Gly-tRF in liver steatosis via antisense inhibitors.
Small RNA high-throughput sequencing
Total RNA was extracted from hepatic tissues of short-term ethanol-fed and pair-fed mice with TRIzol reagent (Invitrogen, Carlsbad, CA, USA). The quality of the RNA samples was evaluated with a NanoDrop 2000c spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and Agilent’s bioanalyzer, and RNA samples were size-fractionated with 15% polyacrylamide gel electrophoresis (PAGE) to collect the 18–40-nt fractions. Sequencing libraries were generated by RT-PCR amplification. The PCR products were purified and sequenced on a HiSeq 2500 sequencing system (BGI Tech Solutions, Shenzhen, China). High-throuhghput sequencing data can be found at the GEO database in NCBI (accession number: GSE126047).
In vivo studies of Gly-tRF inhibitor
Mice were subjected to chronic feeding (1% ethanol for two days followed by 2% for two days, 4% for one week, and 5% for three weeks) according to the previously described approach.4,67 Synthetic Gly-tRF antisense inhibitors “CGUAAGCGAGAAUUCUACCACUGAACCACCAAUGCACAAU” or normal control (modified with 2′-O-Me and cholesterol; RiboBio, Guangzhou, China) (20 nmoles/per mouse) were injected i.p. into ethanol-fed mice twice weekly for the last three weeks. After three weeks, mice were euthanized and the effect of the Gly-tRF inhibitors on the liver was assessed. Hepatic tissues from mice treated with Gly-tRF inhibitors or normal controls (scrambled sequence) were subjected to transcriptome sequencing.
Adeno-associated virus construction and infection
AAV9 was used to construct the shRNAs targeting the C5aR2, Cyp2e1, and Sirt1 genes. AAV9-Sirt1 was developed for overexpression of Sirt1 (GeneChem, Shanghai, China). Recombinant virus (1.5 × 1011 v.g) was diluted in 0.2 mL of a saline solution and injected into the tail vein of mice. One week post injection, the mice were subjected to a short-term binge feeding.
Peptides and chemical reagent
Mice were subjected to short-term binge feeding. During feeding with 5% ethanol, 0.1 mL of 150 μg/mL C3a peptide (pC3a) or Asp peptide (pAsp, also known as C3a-des Arg) was daily admininstered i.p. to the experimental group.8 Saline administration served as the control. On day 10, the mice were sacrificed, and liver tissues and serum were harvested. The C3a and Asp peptides were produced by Nanjing Peptide Industry (Nanjing, China). For the chlormethiazole (CMZ) experiment, CMZ (Sigma, 50 mg/kg body weight) was injected i.p. every other day at the time of 5% ethanol diet feeding.68
Cell culture and transfection
AML12 cells were kindly provided by Stem Cell Bank, Chinese Academy of Sciences, Shanghai, China. The AML12 cells were cultured in Dulbecco’s modified Eagle medium (DMEM-F12) supplemented with 10% fetal bovine serum, 0.1 μM dexamethasone, and insulin-transferrin-selenium. The cells were grown at 37 °C in an atmosphere of 5% CO2. Gly-tRF mimics “GCATTGGTGGTTCAGTGGTAGAATTCTCGC” or normal control (modified with 2′-O-Me and cholesterol) was synthesized by RiboBio (Guangzhou, China). The AML12 cells were transfected with Gly-tRF mimics, Gly-tRF inhibitors, or small interfering RNA (siRNA) (RiboBio, Guangzhou, China) using Lipo3000 (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol.
Histopathologic and biochemical analyses
Fresh liver tissues were frozen, and sections (8-μm thick) were prepared and stained with Oil Red O. Other liver tissues were fixed with 10% neutral formalin and embedded in paraffin, and tissue sections (5-μm thick) were cut and stained with H&E. Histopathological alterations of the liver tissue were observed using a microscope. Plasma ALT and AST levels were measured with an autoanalyzer (ANTECH Diagnostics, Los Angeles, CA, USA).
Immunohistochemistry
The resected liver tissues were fixed overnight with 10% formalin, and paraffin-embedded sections (4-μm thick) were prepared. Sections were then processed for immunohistochemical staining. Antigen retrieval was performed by pressure cooking for 3 min in citrate buffer (pH 6), followed by peroxidase and serum blocking steps. The sections were incubated with goat anti-C3d antibody (R&D Systems, 1:100 dilution) for 2 h at room temperature, followed by antibody detection with an anti-goat ImmPRESS kit (Vector Laboratories). Apoptosis was quantified by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) using a TUNEL kit (Roche, Mannheim, Germany) according to the manufacturer’s instructions. The cell nuclei were counterstained with DAPI (1 mg/mL). The apoptotic cells were labeled green with the TUNEL staining kit. The images were collected using a fluorescent microscope (IX-71; Olympus, Tokyo, Japan).
RNA extraction and qRT-PCR
Total RNA was extracted from hepatic tissues or AML12 cells according to the TRIzol extraction protocol (Invitrogen). Gly-tRFs were assayed according to a previously described method.13 RNA transcript levels were measured by qRT-PCR, using a SYBR Green PCR master mix (Bio-Rad). All experiments were performed in triplicate. The primers used in this study are shown in Supplementary information, Table S1.
Measurement of SOD, GSH, MDA and triglyceride levels and CYP2E1 activity in liver homogenates
The levels of SOD, GSH, MDA and triglyceride in liver tissue homogenates from each group were measured using the corresponding kits (Catalog# A001-3, A006-2, A003-1, and A110-1, respectively) from Nanjing Jiancheng Bioengineering Institute (Nanjing, China) according to the manufacturer’s protocols. CYP2E1 activity was assayed by the rate of oxidation of p-nitrophenol to p-nitrocatechol according to a previously described protocol.68
Western blot analysis
Proteins from hepatic samples or cells were analyzed by standard western blotting. Primary antibodies anti-SREBP1c (ab28481), anti-ANG (ab10600) and anti-CYP2E1 (ab28146) were from Abcam, Cambridge, UK; anti-PPARα (15540-1-ΑP) and anti-SIRT1 (13161-1-AP) were from Proteintech; anti-caspase-3 (#9662), anti-caspase-8 (#8592), anti-caspase-9 (#9504), anti-Bax (#2772), anti-Bcl2 (#3498), anti-STAT3 (#9139), anti-P-STAT3 (#9145), anti-Akt (#4685) and anti-P-Akt (#4060) were from Cell Signaling Technology. Anti-β-actin (66009-1-Ig; Proteintech) was used to normalize the signals.
Serum cytokine and C3a measurements
Serum TNFα, IL-6 and C3a levels were measured using commercially available ELISA kits (R&D Systems) or (CUSABIO, Wuhan, China), following the manufacturer’s instructions.
Plasmid construction and luciferase reporter assay
The 3′ UTR fragment of Sirt1 was amplified using specific primers and cloned into the multiple cloning site of pMir-reporter vector (Promega, Madison, WI, USA). Mutations in the 3′ UTR fragment were generated via PCR-based mutagenesis. The primers used are shown in Supplementary information, Table S1. The AML12 cells were co-transfected with the plasmids and Gly-tRF mimics or inhibitors. After 48 h, the cells were collected and luciferase assays were performed according to the manufacturer’s protocol (Promega).
RIP-qPCR and immunoprecipitation
AML12 cells were transfected with plasmids expressing AGO3-HA or AGO2-HA, or pcDNA3.1. Two days later, the AML12 cells were washed with cold PBS, placed on ice and irradiated with 254 nm UV light for 5 min.69 Next, the cells were collected and lysed in RIP buffer, following the manufacturer’s protocol (Magna RIP kit, Millipore). The cell lysates were then separated by centrifugation at 14,000 rpm for 15 min at 4 °C. Anti-HA antibody (M180-3, MBL) was mixed with magnetic beads coated with protein A/G and incubated with constant rotation for 30 min at room temperature. Subsequently, the anti-HA antibody and protein A/G-coated magnetic beads were mixed with the lysates and incubated at 4 °C for 4 h. The beads were then precipitated using a magnetic separator, followed by six washes with cool RIP buffer. The immunoprecipitated RNA samples were then extracted and reverse transcribed using an RT reagent kit (Invitrogen) with specifc primers, followed by qPCR. The results were normalized to input RNA levels and plotted as fold enrichment relative to that of the IgG control.
The liver tissues of pair-fed or ethanol-fed mice were disrupted using a lysis buffer containing a protease inhibitor mixture (Sigma) for 30 min on ice. The cell lysates were separated by centrifugation at 14,000 rpm for 15 min at 4 °C. Protein G/A-coated microbeads were incubated with the antibody β-arrestin2 (ab54790, Abcam) or IgG for 30 min at room temperature and then washed three times with cold lysis buffer. The microbeads were mixed with the cell lysates following incubation overnight at 4 °C. The immunoprecipitation samples were anlyzed by western blotting. Anti-C5aR2 (A10588, Abclonal) was used to detect endogenous C5aR2.
Data analysis
Data were expressed as the means ± standard deviation (SD). Significant differences between groups were determined by ANOVA, with a Bonferroni correction for continuous variables and multiple groups. Two-tailed student’s t-test was used for the comparison of a normally distributed continuous variable between two groups. P values < 0.05 were considered statistically significant.
References
Gao, B. & Bataller, R. Alcoholic liver disease: pathogenesis and new therapeutic targets. Gastroenterology 141, 1572–85 (2011).
Chick, J. The WHO global strategy to reduce the harmful use of alcohol. Alcohol Alcohol 46, 223 (2011).
Rehm, J. et al. Global burden of disease and injury and economic cost attributable to alcohol use and alcohol-use disorders. Lancet 373, 2223–33 (2009).
Pritchard, M. T. et al. Differential contributions of C3, C5, and decay-accelerating factor to ethanol-induced fatty liver in mice. Gastroenterology 132, 1117–26 (2007).
Roychowdhury, S. et al. An early complement-dependent and TLR-4-independent phase in the pathogenesis of ethanol-induced liver injury in mice. Hepatology 49, 1326–34 (2009).
Dunkelberger, J. R. & Song, W. C. Complement and its role in innate and adaptive immune responses. Cell Res. 20, 34–50 (2010).
Atkinson, C. et al. Targeted complement inhibition by C3d recognition ameliorates tissue injury without apparent increase in susceptibility to infection. J. Clin. Investig. 115, 2444–53 (2005).
He, S. et al. A complement-dependent balance between hepatic ischemia/reperfusion injury and liver regeneration in mice. J. Clin. Investig. 119, 2304–16 (2009).
Marshall, K. M., He, S., Zhong, Z., Atkinson, C. & Tomlinson, S. Dissecting the complement pathway in hepatic injury and regeneration with a novel protective strategy. J. Exp. Med. 211, 1793–805 (2014).
Lee, Y. S., Shibata, Y., Malhotra, A. & Dutta, A. A novel class of small RNAs: tRNA-derived RNA fragments (tRFs). Genes Dev. 23, 2639–49 (2009).
Cole, C. et al. Filtering of deep sequencing data reveals the existence of abundant Dicer-dependent small RNAs derived from tRNAs. RNA 15, 2147–60 (2009).
Li, Z. et al. Extensive terminal and asymmetric processing of small RNAs from rRNAs, snoRNAs, snRNAs, and tRNAs. Nucleic Acids Res. 40, 6787–99 (2012).
Zhong, F. et al. A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes. Nucleic Acids Res. 43, 10474–91 (2015).
Goodarzi, H. et al. Endogenous tRNA-derived fragments suppress breast cancer progression via YBX1 displacement. Cell 161, 790–802 (2015).
Chen, Q. et al. Sperm tsRNAs contribute to intergenerational inheritance of an acquired metabolic disorder. Science 351, 397–400 (2016).
Sharma, U. et al. Biogenesis and function of tRNA fragments during sperm maturation and fertilization in mammals. Science 351, 391–6 (2016).
Gapp, K. & Miska, E. A. tRNA fragments: novel players in intergenerational inheritance. Cell Res. 26, 395–6 (2016).
Schimmel, P. The emerging complexity of the tRNA world: mammalian tRNAs beyond protein synthesis. Nat. Rev. Mol. Cell Biol. 19, 45–58 (2018).
Guzzi, N. et al. Pseudouridylation of tRNA-derived fragments steers translational control in stem cells. Cell 173, 1204–16 (2018).
Zhang, Y. et al. Dnmt2 mediates intergenerational transmission of paternally acquired metabolic disorders through sperm small non-coding RNAs. Nat. Cell Biol. 20, 535–40 (2018).
Keam, S. P. & Hutvagner, G. tRNA-derived fragments (tRFs): emerging new roles for an ancient RNA in the regulation of gene expression. Life 5, 1638–51 (2015).
Fu, Y., Lee, I., Lee, Y. S. & Bao, X. Small non-coding transfer RNA-derived RNA fragments (tRFs): their biogenesis, function and implication in human diseases. Genom. Inform. 13, 94–101 (2015).
Ivanov, P., Emara, M. M., Villen, J., Gygi, S. P. & Anderson, P. Angiogenin-induced tRNA fragments inhibit translation initiation. Mol. Cell 43, 613–23 (2011).
Gebetsberger, J., Wyss, L., Mleczko, A. M., Reuther, J. & Polacek, N. A tRNA-derived fragment competes with mRNA for ribosome binding and regulates translation during stress. RNA Biol. 14, 1364–73 (2017).
Fu, H. et al. Stress induces tRNA cleavage by angiogenin in mammalian cells. FEBS Lett. 583, 437–42 (2009).
Thompson, D. M. & Parker, R. Stressing out over tRNA cleavage. Cell 138, 215–9 (2009).
Saikia, M. et al. Genome-wide identification and quantitative analysis of cleaved tRNA fragments induced by cellular stress. J. Biol. Chem. 287, 42708–25 (2012).
Li, Q. et al. tRNA-derived small non-coding RNAs in response to ischemia inhibit angiogenesis. Sci. Rep. 6, 20850 (2016).
Thompson, D. M., Lu, C., Green, P. J. & Parker, R. tRNA cleavage is a conserved response to oxidative stress in eukaryotes. RNA 14, 2095–103 (2008).
Cederbaum, A. I. Cytochrome P450 2E1-dependent oxidant stress and upregulation of anti-oxidant defense in liver cells. J. Gastroenterol. Hepatol. 21(Suppl 3), S22–S25 (2006).
Galligan, J. J. et al. Oxidative stress and the ER stress response in a murine model for early-stage alcoholic liver disease. J. Toxicol. 2012, 207594 (2012).
Leung, T. M. & Nieto, N. CYP2E1 and oxidant stress in alcoholic and non-alcoholic fatty liver disease. J. Hepatol. 58, 395–8 (2013).
Louvet, A. & Mathurin, P. Alcoholic liver disease: mechanisms of injury and targeted treatment. Nat. Rev. Gastroenterol. Hepatol. 12, 231–42 (2015).
You, M., Liang, X., Ajmo, J. M. & Ness, G. C. Involvement of mammalian sirtuin 1 in the action of ethanol in the liver. Am. J. Physiol. Gastrointest Liver Physiol. 294, G892–G898 (2008).
Schug, T. T. & Li, X. Sirtuin 1 in lipid metabolism and obesity. Ann. Med. 43, 198–211 (2011).
You, M., Jogasuria, A., Taylor, C. & Wu, J. Sirtuin 1 signaling and alcoholic fatty liver disease. Hepatobiliary Surg. Nutr. 4, 88–100 (2015).
Hong, S. et al. Nicotinamide N-methyltransferase regulates hepatic nutrient metabolism through Sirt1 protein stabilization. Nat. Med. 21, 887–94 (2015).
Yin, H. et al. Deletion of SIRT1 from hepatocytes in mice disrupts lipin-1 signaling and aggravates alcoholic fatty liver. Gastroenterology 146, 801–11 (2014).
Ponugoti, B. et al. SIRT1 deacetylates and inhibits SREBP-1C activity in regulation of hepatic lipid metabolism. J. Biol. Chem. 285, 33959–70 (2010).
Zhang, Y. et al. Inhibition of poly(ADP-Ribose) polymerase-1 protects chronic alcoholic liver injury. Am. J. Pathol. 186, 3117–30 (2016).
Huang, C. K. et al. Restoration of Wnt/beta-catenin signaling attenuates alcoholic liver disease progression in a rat model. J. Hepatol. 63, 191–8 (2015).
Petrasek, J. et al. STING-IRF3 pathway links endoplasmic reticulum stress with hepatocyte apoptosis in early alcoholic liver disease. Proc. Natl Acad. Sci. USA 110, 16544–9 (2013).
Zhang, X. J. et al. An ALOX12-12-HETE-GPR31 signaling axis is a key mediator of hepatic ischemia-reperfusion injury. Nat. Med. 24, 73–83 (2018).
He, S. et al. A role for complement in the enhanced susceptibility of steatotic livers to ischemia and reperfusion injury. J. Immunol. 183, 4764–72 (2009).
Zhang, T., Garstka, M. A. & Li, K. The controversial C5a receptor C5aR2: its role in health and disease. J. Immunol. Res. 2017, 8193932 (2017).
DeWire, S. M., Ahn, S., Lefkowitz, R. J. & Shenoy, S. K. Beta-arrestins and cell signaling. Annu. Rev. Physiol. 69, 483–510 (2007).
Lieber, C. S., Leo, M. A., Wang, X. & Decarli, L. M. Effect of chronic alcohol consumption on hepatic SIRT1 and PGC-1alpha in rats. Biochem. Biophys. Res. Commun. 370, 44–48 (2008).
Ajmo, J. M., Liang, X., Rogers, C. Q., Pennock, B. & You, M. Resveratrol alleviates alcoholic fatty liver in mice. Am. J. Physiol. Gastrointest Liver Physiol. 295, G833–G842 (2008).
Haussecker, D. et al. Human tRNA-derived small RNAs in the global regulation of RNA silencing. RNA 16, 673–95 (2010).
Kumar, P., Anaya, J., Mudunuri, S. B. & Dutta, A. Meta-analysis of tRNA derived RNA fragments reveals that they are evolutionarily conserved and associate with AGO proteins to recognize specific RNA targets. BMC Biol. 12, 78 (2014).
Shi, J., Zhang, Y., Zhou, T. & Chen, Q. tsRNAs: the Swiss army knife for translational regulation. Trends Biochem. Sci. 44, 185–9 (2019).
Lu, Y., Wu, D., Wang, X., Ward, S. C. & Cederbaum, A. I. Chronic alcohol-induced liver injury and oxidant stress are decreased in cytochrome P4502E1 knockout mice and restored in humanized cytochrome P4502E1 knock-in mice. Free Radic. Biol. Med. 49, 1406–16 (2010).
Kalant, D. et al. C5L2 is a functional receptor for acylation-stimulating protein. J. Biol. Chem. 280, 23936–44 (2005).
Kalant, D. et al. The chemoattractant receptor-like protein C5L2 binds the C3a des-Arg77/acylation-stimulating protein. J. Biol. Chem. 278, 11123–9 (2003).
Paglialunga, S. et al. Reduced adipose tissue triglyceride synthesis and increased muscle fatty acid oxidation in C5L2 knockout mice. J. Endocrinol. 194, 293–304 (2007).
Gerbal-Chaloin, S. et al. The WNT/beta-catenin pathway is a transcriptional regulator of CYP2E1, CYP1A2, and aryl hydrocarbon receptor gene expression in primary human hepatocytes. Mol. Pharmacol. 86, 624–34 (2014).
Jin, M., Ande, A., Kumar, A. & Kumar, S. Regulation of cytochrome P450 2e1 expression by ethanol: role of oxidative stress-mediated pkc/jnk/sp1 pathway. Cell Death Dis. 4, e554 (2013).
Wlazlo, N. et al. Activated complement factor 3 is associated with liver fat and liver enzymes: the CODAM study. Eur. J. Clin. Investig. 43, 679–88 (2013).
Shen, H., French, B. A., Liu, H., Tillman, B. C. & French, S. W. Increased activity of the complement system in the liver of patients with alcoholic hepatitis. Exp. Mol. Pathol. 97, 338–44 (2014).
Wang, Y. et al. Ethanol-induced cytochrome P4502E1 causes carcinogenic etheno-DNA lesions in alcoholic liver disease. Hepatology 50, 453–61 (2009).
Dilger, K., Metzler, J., Bode, J. C. & Klotz, U. CYP2E1 activity in patients with alcoholic liver disease. J. Hepatol. 27, 1009–14 (1997).
Yano, H. et al. Study of cytochrome P4502E1 mRNA level of mononuclear cells in patients with alcoholic liver disease. Alcohol Clin. Exp. Res. 25, 2S–6S (2001).
Hou, Y. et al. Association of SIRT1 gene polymorphism and its expression for the risk of alcoholic fatty liver disease in the Han population. Hepatol. Int. 12, 56–66 (2018).
Schorn, A. J., Gutbrod, M. J., LeBlanc, C. & Martienssen, R. LTR-retrotransposon control by tRNA-derived small RNAs. Cell 170, 61–71 (2017).
Zhang, Y., Shi, J. & Chen, Q. tsRNAs: new players in mammalian retrotransposon control. Cell Res. 27, 1307–8 (2017).
Bertola, A., Mathews, S., Ki, S. H., Wang, H. & Gao, B. Mouse model of chronic and binge ethanol feeding (the NIAAA model). Nat. Protoc. 8, 627–37 (2013).
Sebastian, B. M. et al. Identification of a cytochrome P4502E1/Bid/C1q-dependent axis mediating inflammation in adipose tissue after chronic ethanol feeding to mice. J Biol. Chem. 286, 35989–97 (2011).
Lu, Y., Zhuge, J., Wang, X., Bai, J. & Cederbaum, A. I. Cytochrome P450 2E1 contributes to ethanol-induced fatty liver in mice. Hepatology 47, 1483–94 (2008).
Macias, S. et al. DGCR8 HITS-CLIP reveals novel functions for the Microprocessor. Nat. Struct. Mol. Biol. 19, 760–6 (2012).
Acknowledgements
This study was supported in part by the State Key Program of the National Natural Science Foundation of China (81430014), the National Natural Science Foundation of China (81660103, 81771674 and 81860654), 111 Project (D17011), the National Institutes of Health (R01DK102912), Guangxi BaGui Scholars, Project of Guangxi Health and Family Planning Commission (S201602), Basic Ability Promotion Project of Guangxi (2017KY0481), Natural Science Foundation of Guangxi (2015GXNSFFA139004, 2016JJB140027), the Project of Traditional Chinese Medicine of Guangxi Health and Family Planning Commission (GZLC16-47), the Project of Guangxi University Collaborative Innovation Center, and Guangxi Digestive Disease Clinical Medical Research Center Construction Project (AD17129027).
Author information
Authors and Affiliations
Contributions
SH directed and supervised the study, and revised the manuscript. FZ designed and performed most of the experiments and wrote the manuscript; ZH, KJ, BL, ZW, GY, HL, CD, BT, CZ, SY, YZ, ZG, SY, HS, and GZ participated in some experiments. ST and XQ provided advice, reagents and revised the manuscript.
Corresponding author
Ethics declarations
Competing interests
Stephen Tomlinson is an inventor on a licensed patent for CR2-targeted complement inhibition. The other authors declare no competing interests.
Supplementary information
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Zhong, F., Hu, Z., Jiang, K. et al. Complement C3 activation regulates the production of tRNA-derived fragments Gly-tRFs and promotes alcohol-induced liver injury and steatosis. Cell Res 29, 548–561 (2019). https://doi.org/10.1038/s41422-019-0175-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41422-019-0175-2
This article is cited by
-
Biological function and clinical application prospect of tsRNAs in digestive system biology and pathology
Cell Communication and Signaling (2023)
-
The monomer TEC of blueberry improves NASH by augmenting tRF-47-mediated autophagy/pyroptosis signaling pathway
Journal of Translational Medicine (2022)
-
Plasma tRNA-derived small RNAs signature as a predictive and prognostic biomarker in lung adenocarcinoma
Cancer Cell International (2022)
-
tRF-3013b inhibits gallbladder cancer proliferation by targeting TPRG1L
Cellular & Molecular Biology Letters (2022)
-
Gly-tRF enhances LCSC-like properties and promotes HCC cells migration by targeting NDFIP2
Cancer Cell International (2021)