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Cryo-EM structure of the human α5β3 GABAA receptor

Cell Researchvolume 28pages958961 (2018) | Download Citation

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Acknowledgements

We acknowledge the use of cryoEM instruments and computing resources in Center for Integrative Imaging of Hefei National Laboratory for Physical Sciences at the Microscale of University of Science and Technology of China, Center of Cryo-Electron Microscopy, Zhejiang University and the Electron Imaging Center for Nanomachines at University of California, Los Angeles. We thank the Bioinformatics Center of the University of Science and Technology of China, School of Life Science, for providing supercomputing resources for this project. This work was supported in part by funds from the Ministry of Science and Technology (Awards 2016YFA0500404 and 2014CB910300 to S.Y. and 2016YFA0501102 to X.Z.), the National Natural Science Foundation of China (Awards 31525001 and 31430019 to S.Y., 31600606 to X.Z. and 31630030 to G.Q.B.), and the Fundamental Research Funds for the Central Universities (to S.Y.). The Electron Imaging Center for Nanomachines at University of California, Los Angeles is supported by grants from NIH (GM071940, S10RR23057, S10OD018111 and U24GM116792) and NSF (DBI-1338135 and DMR-1548924). Structure coordinates and cryo-EM density maps have been deposited in the protein data bank under accession number 6A96 and EMD-6998.

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Author notes

  1. These authors contributed equally: Liu S, Xu L and Guan F.

Affiliations

  1. Life Sciences Institute and School of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310058, P. R. China

    • Si Liu
  2. Life Sciences Institute and Innovation Center for Cell Signaling Network, Zhejiang University, Hangzhou, Zhejiang, 310058, P. R. China

    • Lingyi Xu
    • , Fenghui Guan
    • , Xiang Zheng
    •  & Sheng Ye
  3. Center for Integrative Imaging, Hefei National Laboratory for Physical Sciences at the Microscale, and School of Life Sciences, University of Science and Technology of China (USTC), Hefei, Anhui, 230026, P. R. China

    • Yun-Tao Liu
    •  & Guo-Qiang Bi
  4. School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, 230026, P. R. China

    • Yun-Tao Liu
    •  & Guo-Qiang Bi
  5. CAS Key Laboratory of Brain Function and Disease, University of Science and Technology of China, Hefei, Anhui, 230026, P. R. China

    • Yun-Tao Liu
    •  & Guo-Qiang Bi
  6. Electron Imaging Center for Nanomachines, University of California, Los Angeles, Los Angeles, CA, 90095, USA

    • Yanxiang Cui
  7. Department of Biophysics, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, 310058, P. R. China

    • Qing Zhang
    •  & Xiaokang Zhang
  8. Center of Cryo Electron Microscopy, Zhejiang University, Hangzhou, Zhejiang, 310058, P. R. China

    • Qing Zhang
    •  & Xiaokang Zhang
  9. CAS Center for Excellence in Brain Science and Intelligence Technology, University of Science and Technology of China, Hefei, Anhui, 230026, P. R. China

    • Guo-Qiang Bi
  10. Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, Los Angeles, CA, 90095, USA

    • Z. Hong Zhou
  11. California NanoSystems Institute, University of California, Los Angeles, Los Angeles, CA, 90095, USA

    • Z. Hong Zhou
  12. School of Life Sciences, Tianjin University, 92 Weijin Road, Nankai District, Tianjin, 300072, P. R. China

    • Sheng Ye

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Competing interests

The authors declare no competing interests.

Corresponding authors

Correspondence to Z. Hong Zhou or Xiaokang Zhang or Sheng Ye.

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DOI

https://doi.org/10.1038/s41422-018-0077-8