Neonatal ketone body elevation regulates postnatal heart development by promoting cardiomyocyte mitochondrial maturation and metabolic reprogramming

Chong, Danyang; Gu, Yayun; Zhang, Tongyu; Xu, Yu; Bu, Dandan; Chen, Zhong; Xu, Na; Li, Liangkui; Zhu, Xiyu; Wang, Haiquan; Li, Yangqing; Zheng, Feng; Wang, Dongjin; Li, Peng; Xu, Li; Hu, Zhibin; Li, Chaojun

doi:10.1038/s41421-022-00447-6

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Published: 11 October 2022

Neonatal ketone body elevation regulates postnatal heart development by promoting cardiomyocyte mitochondrial maturation and metabolic reprogramming

Danyang Chong^1,2^na1,
Yayun Gu¹^na1,
Tongyu Zhang²^na1,
Yu Xu¹,
Dandan Bu²,
Zhong Chen²,
Na Xu²,
Liangkui Li³,
Xiyu Zhu⁴,
Haiquan Wang²,
Yangqing Li²,
Feng Zheng²,
Dongjin Wang⁴,
Peng Li³,
Li Xu³,
Zhibin Hu ORCID: orcid.org/0000-0002-8277-5234¹ &
…
Chaojun Li ORCID: orcid.org/0000-0001-9474-7321^1,2

Cell Discovery volume 8, Article number: 106 (2022) Cite this article

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Abstract

Neonatal heart undergoes metabolic conversion and cell cycle arrest preparing for the increased workload during adulthood. Herein, we report that neonatal ketone body elevation is a critical regulatory factor for postnatal heart development. Through multiomics screening, we found that the expression of 3-hydroxy-3-methylglutaryl-CoA synthase 2 (HMGCS2), the rate-limiting enzyme of ketogenesis, was transiently induced by colostrum in the neonatal heart. Hmgcs2 knockout caused mitochondrial maturation defects. Meanwhile, postnatal heart development was compromised and cardiomyocytes reacquired proliferation capacity in Hmgcs2 knockout mice. Consequently, over 40% of newborn Hmgcs2 knockout mice died before weaning. The heart function of surviving Hmgcs2 knockout mice was also impaired, which could be rescued by ketone body supplementation during the suckling stage. Mechanistically, ketone body deficiency inhibited β-hydroxybutyrylation but enhanced acetylation of mitochondrial proteins, which might be responsible for the inhibition of the enzyme activity in mitochondria. These observations suggest that ketone body is critical for postnatal heart development through regulating mitochondrial maturation and metabolic reprogramming.

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Introduction

The cells of various organs in newborns must undergo a series of reprogramming processes to adapt to the postnatal environment. It is not only external factors such as temperature and oxygen that change dramatically after birth, factors of the internal milieu, such as metabolic substrates, are also largely altered after birth. For example, transplacental supplementation with carbohydrates, primarily glucose and lactate, provides energy for myocardial contraction in the fetal heart¹. The acquisition of dietary lipids from colostrum provokes a metabolic shift from anaerobic glycolysis to aerobic fatty acid (FA) beta-oxidation in mitochondria since spontaneous breathing allows the newborn to access more oxygen^2,3,4. This metabolic reprogramming occurs in just a few days after birth.

Along with metabolic reprogramming, the short, rod-shaped mitochondria in the embryonic heart gradually transform into large, round mitochondria that gather around myocardial fibers after birth. The morphological maturation of mitochondria in the heart after birth is essential for the improvement of mitochondrial respiratory capacity and the ability to utilize FA, supplying energy for myocardial contraction in the adult heart^1,5. Furthermore, postnatal cardiac mitochondrial maturation and metabolic reprogramming link together, and any defects in these processes can lead to heart developmental anomalies and even heart failure^5,6,7. Another extraordinary change in the postnatal heart is postnatal development. After birth, mouse cardiomyocytes quickly exit from the cell cycle, become bi-nucleated, and undergo hypertrophic growth⁸. Thus, the mouse heart loses regeneration ability within 1 week after birth⁹. However, the mechanisms underlying these changes are largely unknown.

By comparing the gene expression profiles and proteome data of prenatal and postnatal mouse hearts, we found that significant changes in the expression of ketone body metabolism-related genes occurred during the perinatal stage. Both the mRNA and protein levels of Hmgcs2, a rate-limiting enzyme in ketogenesis, were increased more than 10 fold in the hearts at postnatal day 7 (P7d) compared with those at embryonic day 18.5 (E18.5d). HMGCS2 localizes in mitochondria and catalyzes the critical step of ketogenesis in adult liver when extrahepatic tissues face energy demand in various physiological and pathological states, such as starvation, post-exercise, diabetes, and obesity¹⁰. Through ketogenesis, hepatic mitochondria produce ketone bodies, β-hydroxybutyrate (β-HB), acetoacetate, and acetone, from acetyl-CoA at a rate proportional to FA beta-oxidation. β-HB and acetoacetate can be transported into the circulatory system and oxidized by extrahepatic tissues such as the brain and heart, providing energy for their function¹⁰. Ketogenesis is also evoked by an extremely high-fat diet called ketogenic diet which consists of about 90% fat and 10% protein without carbohydrate^10,11. Immediately after birth, most mammalian newborns are fed with colostrum, which is also a high-fat, low-carbohydrate diet¹².

The above analysis inspired us to explore the relationship among ketogenesis, metabolic transition and the loss of heart regeneration capacity during the neonatal stage. We hypothesized that the high fat in colostrum can stimulate ketogenesis in myocytes, which is critical for mitochondrial maturation, the metabolic transition from anaerobic glycolysis to aerobic FA beta-oxidation in mitochondria, and heart regeneration ability loss.

Results

Metabolic reprogramming and cell cycle arrest in the neonatal mouse heart are revealed by multi-omics analysis

To investigate the genetic and metabolic basis of postnatal heart development, we collected E18.5d, P1d and P7d mouse hearts and performed transcriptomics, proteomics and metabolomics assays. mRNA and protein expression profiles revealed significant changes in metabolic pathways. The expression of most genes involved in FA beta-oxidation and mitochondrial oxidative phosphorylation was largely increased in the neonatal heart (Fig. 1a, b; Supplementary Tables S1 and S2), while the expression of glycolysis- and cell cycle-related genes was significantly decreased (Fig. 1c, d; Supplementary Tables S1 and S2). These expression patterns were further confirmed by real-time quantitative PCR (qPCR) in E18.5d and P7d mouse hearts (Supplementary Fig. S1a–d). KEGG enrichment analysis also showed that the expression of proteins related to FA metabolism, FA degradation and the peroxisome proliferation-activated receptor (PPAR) signaling pathway was largely increased, while the expression of genes involved in cell proliferation, such as cell cycle- and DNA replication-related genes (Fig. 1e), was mostly decreased in neonatal hearts. Gene Ontology (GO) analysis of the proteomics data also suggested that the expression of the mitochondrial component protein (Supplementary Fig. S1e) was largely increased, suggesting the association between mitochondrial maturation and the metabolic pattern transition in the neonatal heart.

**Fig. 1: Metabolic reprogramming and cardiomyocyte cell cycle arrest occur in the neonatal mouse heart.**

Furthermore, the content of glycerol, a triglyceride utilization metabolite, was higher in neonatal hearts than that in fetal hearts (Fig. 1f). However, the level of the glycolysis product, lactate, remained unchanged (Fig. 1g) although the pyruvate level was decreased in neonatal hearts (Fig. 1h), which might be due to an increase in pyruvate metabolism-related gene expression (Fig. 1c; Supplementary Tables S1, S2). A metabolomics study of perinatal hearts indicated that the changes of the metabolites in glucose metabolism were diverse, such as glucose-6-phosphate, phosphoenolpyruvate and lactate (Supplementary Fig. S1f), while the tricarboxylic acid (TCA) cycle remained active since NAD⁺ and NADH levels were augmented during the neonatal stage (Supplementary Fig. S1f and Table S3). On the other hand, as mentioned before, the mRNA levels of most cell cycle marker genes, such as most cyclins and cyclin-dependent kinases were down-regulated (Supplementary Fig. S1d). Moreover, the proportions of BrdU- and phosphorylated histone H3 (pHH3)-positive cardiomyocytes were decreased significantly after birth (Fig. 1i, j; Supplementary Fig. S1g, h), which indicates that the cell cycle of cardiomyocytes is rapidly arrested in the postnatal heart.

The above multiomics analysis suggests the existence of an association among metabolic reprogramming, mitochondrial maturation and cell cycle arrest in the neonatal heart that is critical for the functional transition from the embryonic stage to the adult stage.

Vigorous ketogenesis occurs in neonatal mouse heart

In our omics data, we noticed that another group of metabolic genes, ketone body metabolic genes, displayed a striking expression pattern in the neonatal heart. We found that the mRNA and protein levels of Hmgcs2, the gene encoding the rate-limiting enzyme in ketogenesis¹³, were increased dramatically in the hearts of neonatal mice compared with those of fetal mice (Fig. 2a, b; Supplementary Fig. S2a, b). Furthermore, though the mRNA level of Oxct1, a critical gene responsible for ketone body oxidation, detected by RNA-seq decreased from embryonic to neonatal period, qPCR detection showed that Oxct1 mRNA did not change (Fig. 2a, c), probably because Oxct1 changed less and was susceptible to individual differences or due to the quantification error of RNA-seq caused by the rare wrong alignment between the reads and the reference transcriptome¹⁴. However, both proteomics and western blot revealed that the protein levels of OXCT1 in P7d hearts were higher than those in E18.5d hearts (Fig. 2a; Supplementary Fig. S2a, c). Considering that the increase in HMGCS2 protein expression is much higher than that of OXCT1 from the embryonic to neonatal period, we speculate that ketone bodies accumulate in the neonatal heart. As expected, the metabolomic data indicated that the levels of β-HB in P1d and P7d heart tissues were higher compared with those in E18.5d hearts (Fig. 2d). Examination of Hmgcs2 mRNA and protein from the fetal stage to the adult stage showed a typical transient expression pattern during the suckling stage. Not only in heart, but also in most other tissues, including the lung, brain, kidney, the expression of HMGCS2 was extremely low in the embryonic stage and rose sharply after birth, reaching a peak from P3d to P7d. Then, it gradually decreased and disappeared after weaning (P21d) in these organs except the liver and spleen (Fig. 2e; Supplementary Fig. S2d, e).

**Fig. 2: Ketogenesis is enhanced in neonatal mouse heart.**

Moreover, HMGCS2 was not detected in either the atria or ventricles of embryonic hearts and was massively expressed in the ventricular wall but not in the atria of P3d and P7d mouse hearts (Supplementary Fig. S2f). Moreover, HMGCS2 was preferred to express in right ventricle and epicardium but it was rarely expressed in the left ventricle and interventricular septum. The contents of β-HB in heart tissues were increased after birth, reached a maximum level at P7d and then decreased after weaning (P21d) (Fig. 2f). Interestingly, the ketone body level in the blood was above 1 mM during all suckling stages, comparable to the levels under some extreme physiological conditions such as prolonged exercise and overnight fasting¹⁰, and decreased to the normal physiological level by P56d (Fig. 2g). We also found that the mRNA and protein levels of Oxct1 were dramatically increased after suckling (Fig. 2h; Supplementary Fig. S2g, h), which suggests that the heart can use ketone bodies as energy substrates after suckling.

Colostrum FA induces transient massive expression of Hmgcs2 in neonatal mouse heart

Then, we explored which factor is responsible for Hmgcs2 induction during the neonatal stage. There are two significant environmental alterations during perinatal period: the increase in oxygen concentration in the blood because of spontaneous respiration and the introduction of dietary lipids through colostrum^12,15. Ketone bodies are normally produced at a rate proportional to FA oxidation¹⁰. We found that the expression of Hmgcs2 was detectable during all suckling periods and disappeared soon after weaning at approximately the 3^rd week after birth. Thus, we hypothesized that colostrum promotes the rapid expression of Hmgcs2 in the neonatal heart. Cidea is a lipid droplet-associated protein and is abundantly expressed in lactating mammary glands. The deficiency of Cidea reduces milk lipid secretion¹⁶. We detected the protein levels of HMGCS2 in pups from Cidea^−/− females and wild-type males and found that there was no HMGCS2 in the heart tissue of the pups of Cidea^−/− females (Fig. 3a; Supplementary Fig. S3a). Thus, the HMGCS2 expression in the postnatal heart was not able to be induced by lipid-deficient maternal milk. We isolated neonatal rat ventricular myocytes (NRVMs) from the hearts of P0.5d rats that did not suckle milk. Following FA (palmitate (PA): oleate (OA) = 1:1) treatment, we found that both the mRNA and protein levels of Hmgcs2 in NRVMs were markedly enhanced by FA in a time- (Fig. 3b, c; Supplementary Fig. S3b) and dose- (Fig. 3d, e; Supplementary Fig. S3c) dependent manner. When we withdrew FA from the culture medium, the protein level of HMGCS2 gradually decreased beginning on the 4^th day, and almost no HMGCS2 was found after the removal of FA for 6 days (Fig. 3f; Supplementary Fig. S3d). Moreover, FA did not induce OXCT1 expression (Fig. 3g–j; Supplementary Fig. S3e, f), which indicates that FA in colostrum is critical for the transient expression of HMGCS2 but not OXCT1. We also examined the relationship between hypoxia and Hmgcs2 expression by treating NRVMs with CoCl₂ to imitate hypoxic conditions. CoCl₂ did not affect HMGCS2 expression (Supplementary Fig. S3g, h). All the data indicate that the abundant FA in colostrum is responsible for the induction of Hmgcs2 expression during the suckling period.

**Fig. 3: FA in colostrum stimulates transiently abundant *Hmgcs2* expression in neonatal mouse heart.**

Hmgcs2 and ketone body β-HB are essential for mitochondrial maturation in neonatal mouse heart

To determine the effect of massive Hmgcs2 expression and ketone body on the status of cardiomyocytes in neonatal hearts, we inactivated Hmgcs2 gene in all tissues by ubiquitously expressing EIIA-Cre (Supplementary Fig. S4a, b). The levels of β-HB in both blood (Fig. 4a) and heart tissues (Fig. 4b) were decreased dramatically. Then we assessed the gene expression profiles in the hearts of neonatal wild-type (WT) and Hmgcs2 knockout (KO) mice. GSEA indicated that Hmgcs2 deletion blocked the increased expression of genes in neonatal hearts, such as mitochondrial oxidative phosphorylation- and electron transport chain-related genes (Fig. 4c; Supplementary Fig S4c, d). Meanwhile, the morphology of mitochondria was dramatically altered with disorganized cristae (Fig. 4d) and an increase in the mitochondrial area (Supplementary Fig. S4e). β-HB can significantly alleviate the abnormal mitochondrial morphology caused by Hmgcs2 knockout, although mitochondrial swelling was not rescued by β-HB (Fig. 4d; Supplementary Fig. S4e). Moreover, lipid droplets were observed in KO mouse hearts (Fig. 4d, star). BODIPY staining also revealed lipid droplet accumulation in KO mouse hearts, which could be alleviated by β-HB (Supplementary Fig. S4f, g). Further functional examination of isolated mitochondria indicated that Hmgcs2 knockout considerably reduced the mitochondrial oxygen consumption rate (OCR) of neonatal mouse hearts, and β-HB could significantly improve the mitochondrial function inhibited by Hmgcs2 knockout (Fig. 4e, f). Meanwhile, Hmgcs2 knockout did not affect mitochondrial DNA copy number (Supplementary Fig. S4h). Thus, Hmgcs2 and ketone body β-HB are essential for mitochondrial maturation in neonatal mouse hearts.

**Fig. 4: Deletion of ketone bodies disrupts mitochondrial maturation in neonatal mouse heart.**

To further determine whether ketone bodies produced in the heart regulate mitochondrial maturation, we examined the effect of cardiac-specific knockout of Hmgcs2 on mitochondrial maturation in the heart. Thus, we constructed the heart-specific conditional Hmgcs2 knockout (H-cKO) mice. The mRNA and protein levels of Hmgcs2 in the heart were significantly reduced in H-cKO mice (Supplementary Fig. S5a, b). Cardiomyocyte-specific Hmgcs2 knockout had no significant effect on ketone body levels in the blood (Fig. 4g), possibly because ketogenesis takes place mainly in the liver. The ketone body contents were decreased by approximately 30% in the heart tissues of cardiomyocyte-specific Hmgcs2 knockout mice compared with those of WT mice (Fig. 4h). Surprisingly, we found that the morphology of mitochondria in the heart was not altered (Fig. 4i), although a decreased mitochondrial area was observed (Supplementary Fig. S5c).

The liver is the main organ producing ketone bodies in the adult body, which can be absorbed and utilized by the heart¹⁰. Therefore, cardiomyocytes could uptake liver-derived ketone bodies from blood although Hmgcs2 was deleted in the heart, which resulted in a large amount of residual ketone bodies in the hearts of myocardial-specific Hmgcs2 knockout mice (Fig. 4h). Thus, this limited ketone body decrease might not cause the change of mitochondrial morphology. To confirm hepatic contribution to ketone body levels in postnatal mouse hearts, we further obtained liver-specific conditional Hmgcs2 knockout (L-cKO) mice. The mRNA and protein levels of Hmgcs2 in the liver were significantly reduced in L-cKO mice (Supplementary Fig. S5d, e). It was found that liver-specific knockout of Hmgcs2 resulted in a decrease in ketone body levels in the blood (Fig. 4j), but did not affect ketone body levels in the heart tissue (Fig. 4k). Thus, there was no significant change in mitochondrial morphology and size in the hearts of L-cKO mice, compared with those of WT mice (Fig. 4l; Supplementary Fig. S5f). Thus, liver-specific deletion of Hmgcs2 does not influence ketone bodies and mitochondrial maturation in neonatal mouse hearts.

Taken together, the effects of Hmgcs2 deficiency on mitochondrial morphology and function indicate that Hmgcs2 and ketone body β-HB is essential for mitochondrial maturation in neonatal mouse hearts.

Hmgcs2 and ketone body β-HB regulate FA-stimulated metabolic reprogramming in neonatal mouse heart

The energy for the fetal heart is supplied mainly by carbohydrates (especially lactate and glucose) from the placenta. Following birth, exposure to lipid-rich colostrum makes it possible for cardiomyocytes to utilize more efficient FA to produce ATP through FA oxidation and oxidative phosphorylation in mitochondria². We found that FA stimulation largely enhanced lipid metabolism in the neonatal heart, since the expression of genes responsible for lipid uptake into cells (Cd36, Fabp3), lipid uptake into mitochondria (Cpt1b) and beta-oxidation (Acadl and Acadm) was significantly augmented (Fig. 5a), while the expression of genes responsible for glucose uptake (Slc2a4) and metabolism (Hk2, Pfkm, Pkm) was not altered (Fig. 5b). Extracellular metabolic flux analysis indicated that FA stimulation largely enhanced the OCR of cardiomyocytes, but this effect was blocked by Hmgcs2 knockdown (Fig. 5c; Supplementary Fig. S6a–d). FA-enhanced ATP production was also decreased by Hmgcs2 knockdown (Supplementary Fig. S6e). Hmgcs2 deletion also largely decreased the OCR of cardiomyocytes in the neonatal heart, which could be rescued by β-HB (Fig. 5d; Supplementary Fig. S6f). On the other hand, Hmgcs2 deletion had no significant effect on glycolysis (Fig. 5e; Supplementary Fig. S6g). The lactate, pyruvate and ATP levels were also similar between WT and KO hearts (Fig. 5f–h).

**Fig. 5: *Hmgcs2* and ketone body β-HB are essential for FA-stimulated metabolic reprogramming in neonatal mouse heart.**

To further clarify the effect of Hmgcs2 on the ability of cardiomyocytes to utilize metabolic substrates (glucose and FA), we isolated cardiomyocytes from neonatal WT and KO mice and examined metabolic capacity utilizing FA or glucose. Extracellular flux analysis was performed to determine the fuel capacity of FA or glucose by detecting the OCR of a specific fuel when other fuel pathways are inhibited. It was found that Hmgcs2 knockout had no effect on glucose oxidation capacity but resulted in reduced FA oxidation capacity in cardiomyocytes (Fig. 5i, j). These results are consistent with the previous data that Hmgcs2 knockout caused lipid accumulation but did not affect pyruvate and lactate levels in mouse hearts (Fig. 5f, g; Supplementary Fig. S4f, g). Therefore, Hmgcs2 deletion disrupts lipid metabolism and mitochondrial maturation without influencing glucose metabolism in the postnatal heart.

To determine whether the physiological state of the heart of KO mice is close to that of the embryonic heart, we examined the expression of the embryonic marker genes that are strongly expressed in the embryonic heart and are responsible for embryonic heart development. We found that Hmgcs2 deletion led to the increase of the expression of Nppa, Nppb, and Myh7 rather than Hand1, Msx2 and Hey2 (Fig. 5k–p). Considering that Nppa, Nppb and Myh7 are not only embryonic marker genes but also markers of cardiac hypertrophy, we infer that Hmgcs2 knockout causes cardiac stress, but does not affect cardiac development. Taken together, all these data suggest that elevations in the expression of Hmgcs2 and its product β-HB in the neonatal heart are responsible for the metabolic maturation of FA oxidation in the neonatal heart stimulated by FA.

Neonatal elevation of ketone bodies is critical for postnatal heart development and heart function

Next, we explored the consequences of Hmgcs2 deletion and ketone body depletion during the neonatal stage. We found that only approximately 60% of newborn KO mice survived beyond the suckling period (P21d) (Fig. 6a). When we supplied β-HB during the whole suckling period, the survival percentage increased to 80% (Fig. 6a). Further examination indicated that the heart function of surviving KO mice was largely impaired (Fig. 6b). The ejection fraction (Fig. 6c) and fractional shortening (Fig. 6d) of surviving KO mice were significantly lower than those of WT mice. The anatomic study showed that the heart/body weight ratio showed no significant difference between KO and WT mice (Supplementary Fig. S7a–c), and there were no significant structural differences except for a slight decrease in ventricular wall diameter in the KO mouse hearts (Supplementary Fig. S7d). WGA staining revealed that the cardiomyocyte area was significantly reduced in the ventricular wall of KO mouse hearts (Fig. 6e, f). Moreover, the decrease in cardiac function of KO mouse hearts was alleviated by supplementation with β-HB during the whole suckling period (Fig. 6b–d). However, the structural defects and decreased cardiomyocyte area of KO mouse hearts were not alleviated by β-HB supplementation (Fig. 6e, f; Supplementary Fig. S7d). We also tested the effect of Hmgcs2 in the liver on the heart function to figure out whether the liver-derived ketone body regulates postnatal heart function. It was found that liver-specific Hmgcs2 knockout does not influence the heart function of postnatal mice (Supplementary Fig. S7e–g). All these data suggest that a shortage of ketone body stimulation during the neonatal stage results in postnatal heart functional defects.

**Fig. 6: Neonatal elevation of ketone bodies regulates postnatal heart development and heart function.**

On the other hand, one of the important events in postnatal heart development is the gradual loss of regeneration ability of the neonatal heart, which occurs within one week after birth⁹. By pHH3 immunofluorescence staining and BrdU incorporation assay, we showed that cardiomyocyte proliferation did not stop in the neonatal hearts of Hmgcs2-deficient mice, but it did stop upon administration of β-HB (Fig. 6g; Supplementary Fig. S7h–j). We found that FA in colostrum stimulated the expression of Hmgcs2 and enhanced mitochondrial function in NRVMs (Figs. 3, 5). Thus, through CCK-8 detection and immunofluorescence staining of pHH3 and proliferating cell nuclear antigen (PCNA), we examined the effect of FA on cardiomyocyte proliferation and found that FA blocked the proliferation of H9C2 cells (Fig. 6h; Supplementary Fig. S7k–m). Moreover, β-HB had a similar effect as FA on cardiomyocyte proliferation (Fig. 6i; Supplementary Fig. S7n–p).

Furthermore, we used nicotinamide riboside (NR) and omaveloxolone (OMA) to enhance mitochondrial activity (Supplementary Fig. S8a, b). Immunofluorescence staining of pHH3 and PCNA showed that cardiomyocyte proliferation was blocked by NR and OMA (Fig. 6j; Supplementary Fig. S8c–i), suggesting that FA-regulated mitochondrial maturation was also related to the inhibition of cardiomyocyte proliferation. Therefore, our data suggest that Hmgcs2 expression and ketone body production in the neonatal heart are beneficial for postnatal heart development and function.

Hmgcs2 and ketone body β-HB regulate mitochondrial maturation possibly by mediating protein β-hydroxybutyrylation and acetylation in the neonatal heart

Previous reports have shown that ketogenesis can affect mitochondrial protein lysine acetylation (K-ac) and β-hydroxybutyrylation (K-bhb)^17,18. Therefore, we isolated mitochondria from perinatal mouse hearts and found that the K-ac of the large-molecular-weight mitochondrial proteins was increased in neonatal hearts compared with that in embryonic hearts. However, the K-ac of the small-molecular-weight mitochondrial proteins did not change (Fig. 7a; Supplementary Fig. S9a). Moreover, the K-bhb of mitochondrial proteins was largely enhanced in the mouse hearts from the embryonic to neonatal period (Fig. 7b; Supplementary Fig. S9b). We then deleted Hmgcs2 to deplete ketone bodies and found that mitochondrial protein K-ac was increased (Fig. 7c; Supplementary Fig. S9c), while mitochondrial protein K-bhb was largely decreased (Fig. 7d; Supplementary Fig. S9d) in Hmgcs2-deficient hearts. Acetyl-CoA and β-HB are donors for protein K-ac and K-bhb, respectively^19,20. Hmgcs2 deficiency decreased ketone body levels (Fig. 4b) and led to the accumulation of acetyl-CoA in neonatal hearts (Fig. 7e). Thus, we wondered whether mitochondrial protein K-bhb and K-ac can influence each other. Mitochondria of neonatal mouse hearts were isolated and treated with β-HB or acetyl-CoA. As expected, β-HB promoted mitochondrial protein K-bhb in Hmgcs2-deleted hearts (Fig. 7f; Supplementary Fig. S9e). Interestingly, the K-ac of mitochondrial proteins was blocked by β-HB (Fig. 7f; Supplementary Fig. S9f). On the other hand, acetyl-CoA enhanced mitochondrial protein K-ac (Fig. 7g; Supplementary Fig. S9g) but inhibited K-bhb (Fig. 7g; Supplementary Fig. S9h) in WT hearts. The succinate dehydrogenase complex flavoprotein subunit A (SDHA), isocitrate dehydrogenase 2 (IDH2) and malate dehydrogenase 2 (MDH2) are critical enzymes in the TCA cycle and are modified by K-ac and K-bhb^19,21. In Hmgcs2-deficient hearts, the K-bhb of these three enzymes was decreased (Fig. 7h; Supplementary Fig. S9i). However, the K-ac of IDH2, SDHA and MDH2 was up-regulated in KO mouse hearts compared to that in WT mouse hearts (Fig. 7i; Supplementary Fig. S9j). In addition, β-HB could promote K-bhb of IDH2, SDHA rather than MDH2 in KO mouse hearts (Supplementary Fig. S9k). β-HB could also inhibit the increased K-ac of IDH2 and SDHA in KO mouse hearts (Supplementary Fig. S9l). Furthermore, the enzyme activity of IDH and SDH, but not MDH, was decreased in Hmgcs2-deleted hearts and recovered by β-HB supplementation (Fig. 7j–l). The mitochondrial respiratory capacity in Hmgcs2-deleted hearts was also improved by β-HB (Fig. 7m; Supplementary Fig. S10a).

**Fig. 7: Mitochondrial protein β-hydroxybutyrylation and acetylation may be responsible for the regulation of mitochondrial maturation by ketone body β-HB.**

To further determine the contribution of K-bhb modification induced by β-HB to the activities of IDH, SDH and MDH, the activities of mitochondrial IDH, SDH and MDH in β-HB-treated 293T cells were detected. It was found that the activities of IDH and SDH but not MDH were enhanced by β-HB treatment (Supplementary Fig. S10b–d). Moreover, we mutated the specific lysine residue modified by K-bhb in IDH2 and SDHA²². We found that mutations of lysine179 (K179) to arginine (K179R) in IDH2 and lysine263 (K263) to arginine (K263R) in SDHA, which prevented K-bhb, inhibited the enzyme activity of IDH and SDH (Fig. 7n, o). The K to R mutations in IDH2 and SDHA also decreased mitochondrial respiratory capacity (Fig. 7p, q; Supplementary Fig. S10e, f). However, since we cannot rule out the effects of other protein modifications or allosteric effects on enzyme activity, we conclude that ketone body β-HB can promote mitochondrial metabolic enzyme activity and mitochondrial respiratory function, possibly by not only affecting the K-bhb and K-ac modification of mitochondrial proteins, but also other protein modifications and allosteric effect of β-HB. Regarding cell proliferation, PCNA expression was not affected by K to R mutation. However, the protein level of pHH3 was increased by K to R mutation even though the difference was not statistically significant (P = 0.062 between WT-IDH2 and IDH2-K179R, P = 0.084 between WT-SDHA and SDHA-K263R, respectively. Figure 7r; Supplementary Fig. S10g–h). To exclude the effect of HMGCS2 or ketone body β-HB on the mRNA and protein levels of IDH2, SDHA and MDH2, we detected the mRNA and protein levels of IDH2, SDHA and MDH2, and found that there was no significant difference in the mRNA and protein levels of IDH2, SDHA and MDH2 between WT and KO mouse hearts (Supplementary Fig. S10i, j). Taken together, the results indicate that ketone body β-HB regulates mitochondrial enzyme activity, which might be dependent on mitochondrial protein K-bhb and K-ac (Supplementary Fig. S11).

Discussion

Organ development continues after birth. For example, the nervous system undergoes myelination and synaptogenesis during the postnatal stage²³, and the heart also starts hypertrophic growth to prepare for increasing contraction workload during adulthood^24,25. It has been reported that exposure to famine in infants leads to increased susceptibility to coronary heart disease, hypertension or diabetes in adulthood^26,27,28. However, the underlying mechanism in postnatal development remains largely unknown. Herein, our study indicated that Hmgcs2-controlled ketone body β-HB might be the important molecule that regulates postnatal heart development by promoting cardiomyocyte mitochondrial maturation and metabolic reprogramming.

External environmental factors such as temperature and oxygen change dramatically during the perinatal stage, and factors related to the internal milieu, such as metabolic substrates and related endocrine hormones, are largely altered after birth. FA levels in the blood are increased immediately after birth due to the consumption of lipid-rich colostrum²⁹. FA activates PPAR signaling, permitting the transcription of genes related to FA metabolism, such as FA uptake- and beta-oxidation-related genes^30,31 as well as ketogenic genes. Ketogenesis occurs at rates proportional to FA oxidation in hepatic mitochondria¹⁰. We demonstrated that increased FA levels after birth induced Hmgcs2 expression in cardiomyocytes, which regulated postnatal heart development and cardiac functional maturation. When Hmgcs2 was deleted to decrease ketone body levels, approximately 40% of mice died before weaning. Sudden cardiac death (SCD) is a rare clinical encounter, but autopsy-negative SCD without heart structure defects is more common in pediatric patients than in adult patients. Recently, sudden arrhythmic death syndrome (SADS) and sudden unexpected death in epilepsy (SUDEP) have emerged as etiologies of nonstructural heart defect SCD in children and adults³². However, we still do not know how SADS and SUDEP developed in these individuals. The early death of Hmgcs2-deleted mice and the cardiac function defects of surviving mice suggest that postnatal heart development regulated by ketone body might be related to the etiology of SCD.

We found in Fig. 2 that only HMGCS2 in the liver and spleen was consistently expressed after birth. High abundance of HMGCS2 expression in liver is reasonable since liver is responsible for ketone body production in adult stage¹⁰. The postnatal spleen continues to develop and gradually improves its immune function³³. After birth, aerobic respiration provides most of the energy required by the body. In this work, we found that ketone bodies are essential for mitochondrial maturation after birth. Moreover, a previous study found that ketone bodies can promote the development of memory T cells³⁴. Thus, the persistent expression of HMGCS2 in the postnatal spleen may be related to the postnatal development of the spleen.

HMGCS2 was highly expressed in the right ventricle and epicardium but it was rarely expressed in the left ventricle and interventricular septum. We infer that there might be some relationship between HMGCS2 expression pattern and heart function. Most of the energy required for healthy adult mammalian heart contraction comes from fatty acids, with the remainder being derived from glycolysis³⁵. Although no study has found a relationship between heart failure and HMGCS2 expression, a failing heart has a reduced ability to utilize fatty acids and tends to utilize ketone bodies³⁶. In addition, compared with the left ventricle, the right ventricle has a weaker systolic function and has a relatively lower demand for fatty acid utilization³⁷. Moreover, the epicardium has no systolic function. Therefore, we speculate that the high expression of HMGCS2 in the right ventricle and epicardium may be related to weak heart function.

Although the predominant pathways of energy production in the heart are FA catabolism and glucose/lactate catabolism, cardiomyocytes can also utilize ketone bodies¹⁰. The exciting finding of ketone metabolism suggests that failing human hearts preferentially switch to ketone body utilization^38,39,40. An increasing number of studies have supported the notion that ketone bodies are not only metabolic fuel for cardiac mitochondrial oxidation but also important regulatory molecules that play a role in both signal transduction and epigenetic modification^10,41. Here, we showed that ketone body deficiency caused by Hmgcs2 knockout blocked postnatal mitochondrial maturation by directly modifying mitochondrial proteins and downregulating their enzymatic activity. The impairment of mitochondrial function led to a decrease in FA oxidation in cardiomyocytes. Thus, we observed lipid accumulation in Hmgcs2 knockout hearts. Consistent with our study, a study on human diseases with HMGCS2 mutations revealed that mutations in HMGCS2 led to symptoms of fatty liver and hepatomegaly in patients⁴², which might be because that the loss of ketogenic function of HMGCS2 blocked the metabolic flow channeled from acetyl-CoA to ketone bodies and thus inhibited lipid oxidation. A recent study showed that inhibiting FA oxidation can promote cardiomyocyte proliferation after birth⁴³. We did find that ketone body inhibited cardiomyocyte proliferation after birth, possibly through regulation of FA metabolism in the neonatal heart. Thus, our observation suggests that FA in colostrum-stimulated ketogenesis is critical for heart functional maturation or postnatal development.

Since the expression of ketone body oxidative genes such as Oxct1 is not elevated by FA, the ketone bodies whose synthesis is increased by Hmgcs2 might not be metabolic fuel but important regulatory molecules²⁰. Here, we revealed that the modification of mitochondrial proteins was affected by Hmgcs2 deletion: protein K-bhb was inhibited, while protein K-ac was enhanced by Hmgcs2 knockout in neonatal hearts. It has been reported that approximately 60% of mitochondrial proteins contain lysine acetylation sites, and K-ac inhibits the enzyme activity of most mitochondrial proteins¹⁹. Ketogenesis deficiency in the liver leads to hepatic mitochondrial dysfunction and lipid accumulation during the neonatal period due to mitochondrial protein hyperacetylation⁴⁴. Moreover, a recent study indicated that K-bhb can enhance the enzyme activity of citrate synthase¹⁸. However, this study did not explore the effect of K-bhb on the other enzymes in mitochondria and the relationship between K-bhb and K-ac. Our work indicates that the activity of various mitochondrial enzymes such as IDH and SDH relies on K-bhb. Furthermore, we found that β-HB inhibited mitochondrial protein K-ac, while acetyl-CoA inhibited mitochondrial protein K-bhb. This suggests that K-bhb and K-ac of mitochondrial proteins might compete. The balance of protein K-bhb and K-ac might regulate the functions of mitochondrial proteins, such as IDH and SDH, and then affect mitochondrial maturation. However, even though K to R mutation inactivated protein K-bhb¹⁸, whether K-bhb and K-ac compete at the same lysine sites of mitochondrial proteins needs more examination in the future.

It has been reported that cardiomyocytes of failed hearts generally have a reduced ability to utilize FA and thus tend to absorb and utilize ketone bodies^36,45. β-HB intervention could be used to maintain heart function in heart failure patients⁴⁶. Under conditions of ischemia or reperfusion injury, a ketogenic diet or ketone bodies have cardioprotective effects, possibly because ketone bodies treatment increases the abundance of cardiac mitochondria^47,48,49,50. Since elevations in ketone bodies are beneficial to postnatal heart function, our study suggests that supplementation with ketone bodies may alleviate abnormal heart development or function in babies after birth. Furthermore, we speculate that ketone body deficiency during the postnatal stage might be related to the incidence of sudden cardiac death in infants and adolescents⁵¹. Thus, ketone body supplementation may also be an effective treatment for preventing sudden cardiac death.

In conclusion, we have revealed that transient ketone body elevation in the postnatal heart can enhance K-bhb and inhibit K-ac of mitochondrial proteins, which might be essential for mitochondrial maturation and cardiac function in postnatal mice.

Materials and methods

Animals

C57BL/6J mice were obtained from GemPharmatech Co., Ltd. (Jiangsu, China). Cidea knockout mice were obtained from Peng Li laboratory in Tsinghua University. CRISPR/Cas9 system was used to make Hmgcs2-floxed mice (Hmgcs2^fl/fl) mice. In detail, Cas9 mRNA, sgRNA and donor were co-injected into zygotes. sgRNA directed Cas9 endonuclease cleavage in intron 1–2 and intron 2–3, and created a double-strand break. Such breaks were then repaired, and loxp sites were inserted into intron 1–2 and intron 2–3 respectively by homologous recombination. The sequence between two loxp sites will be deleted in specific tissues or cells when tissue-specific promoter drives the expression of Cre. Systemic Hmgcs2 knockout mice were generated by crossing Hmgcs2^fl/fl mice with EIIA-Cre transgenic mice. H-cKO mice were generated by crossing Hmgcs2^fl/fl mice with cTnT-Cre transgenic mice. L-cKO mice were generated by crossing Hmgcs2^fl/fl mice with Alb-Cre transgenic mice. All animals were housed in an environment with strictly controlled temperature and humidity under a normal 12-h light/dark cycle and were fed ad libitum. All animal experiments were carried out according to the guidelines of the Animal Care and Use Committee of Nanjing Medical University in Nanjing, China.

The hearts of WT and Hmgcs2 knockout mice at P3d were collected for transcriptomic analysis. C57BL/6J mice at E18.5d, P1d and P7d used for multi-omics analysis were obtained from the Nanjing Medical University animal care unit (Nanjing, China). The mouse hearts were collected for transcriptome, proteome and metabolome analysis. Each time point had three biological replicates.

Neonatal mice were treated with saline or β-HB (400 mg/kg/day) through i.p. from P3d to P5d or from P3d to P21d. 1 hour before the mice were sacrificed, neonatal mice were treated with BrdU (50 mg/kg) through i.p.

Cell culture

The hearts of neonatal (0.5-day-old) Sprague–Dawley rats were dissociated and digested with trypsin and type II collagenase (Gibco, USA) to obtain NRVMs. The NRVMs were attached to the bottom of the cell culture dish 48 h after plating. NRVMs or H9C2 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich, cat D7777, USA) supplemented with 10% fetal bovine serum (FBS, Gibco, USA), 100 U/mL penicillin and 100 μg/mL streptomycin at 37 °C with 5% CO₂. The method used to isolate NMVMs was the same as that used to isolate NRVMs.

siRNA was transfected into NRVMs using Lipofectamine 2000 reagent (Invitrogen, cat 11668019, USA) for 48 h according to the manufacturer’s instructions.

FA (palmitate (PA): oleate (OA) = 1:1, 200 μM) was used to treat NRVMs for indicated times, then mRNA and protein levels of HMGCS2 were detected. Both PA and OA were dissolved in 10% BSA.

Chemicals

Palmitate (cat P5585), oleate (cat O7501), FA-free bovine serum albumin (BSA, cat A0281), CoCl₂ (cat C8661), β-HB (cat H0261), BrdU (cat B5002) and Ac-coA (cat A2181) were purchased from Sigma-Aldrich (USA). NR (cat S2935) and OMA (cat S7672) were purchased from Selleck (China).

Measurement of β-HB, lactate, pyruvate, triglycerides, and ATP

The content of β-HB in blood was detected by a blood ketone tester (Keto Mojo, USA). The content of β-HB in heart tissues was detected using a β-Hydroxybutyrate Colorimetric Assay Kit (BioVision, cat K632-100, USA) according to the manufacturer’s instructions. The lactate, pyruvate, and triglyceride levels in heart tissues were measured using a detection kit purchased from Nanjing Jiancheng Bioengineering Institute (cat A019-2, A081, and A110-1, China) according to the manufacturer’s instructions. The ATP content in heart tissues was detected using a detection kit purchased from Beyotime (cat S0026, China) according to the manufacturer’s instructions.

Echocardiographic measurements

The mice were anesthetized with isoflurane. Then, cardiac function was assessed by transthoracic two-dimensional guided M-mode echocardiography with a Vevo 2100 (VisualSonics, Canada).

RNA isolation and qPCR

Total RNA was isolated from mouse hearts or cells using TRIzol reagent (Takara, cat 9109, Japan) according to the manufacturer’s instructions. The concentration of total RNA was measured with a NanoDrop 2000 (Thermo Fisher Scientific, USA). Then, PrimeScript™ RT Master Mix (Takara, Japan) was used to reverse-transcribe 1 μg of RNA into cDNA according to the manufacturer’s instructions.

qPCR was performed with ChamQ Universal SYBR qPCR Master Mix (Q711, Vazyme Biotech Co., Ltd, China) on a ViiA 7 Real-Time PCR System (Applied Biosystems, USA). The relative mRNA levels were normalized to those of Rplp0 (mouse) or Rn18s (rat). The primer sequences are listed in Supplementary Table S4.

Mitochondrial genome quantification

Total DNA of the hearts was extracted using Phenol-Chloroform-Isoamylol (Solarbio, P1012, China). mtDNA content was assessed by qPCR using primers specific for mitochondrial-encoded genes (mt-Co1, mt-Dloop, mt-Atp6) and normalized to nuclear DNA content (a specific locus on mouse chromosome 6). Primer sequences are listed in Supplementary Table S4.

Histological analysis, immunohistochemistry, and immunofluorescence staining

Mouse hearts from different developmental stages were collected and routinely fixed in 4% paraformaldehyde (PFA) overnight, dehydrated in gradient ethanol, embedded in paraffin wax, and sectioned into 5 μm slices.

For histological analysis, the paraffin sections of heart tissues were stained with hematoxylin and eosin (H&E) according to a standard protocol.

For immunohistochemistry and immunofluorescence staining, the paraffin sections were dewaxed, rehydrated, and boiled in citrate buffer (pH 6.0) for antigen retrieval. Then, the sections were permeabilized with 0.1% Triton X-100, blocked with 10% goat serum and incubated with the indicated primary antibodies overnight at 4 °C. Afterward, the slides were washed with PBS 3 times, incubated with secondary antibodies for 1–2 h at room temperature and then washed with PBS 3 times. Immunohistochemical detection was performed with a DAB substrate kit (ZSGB-BIO, cat ZLI-9018, China). A FluoView™ FV3000 confocal microscope (Olympus, Japan) was used to observe the immunofluorescence staining.

The primary antibodies used in immunohistochemistry and immunofluorescence staining are listed in Supplementary Table S5.

Transmission electron microscopy

Mouse hearts were fixed in 4% glutaraldehyde, postfixed in 1% osmium tetroxide, dehydrated in gradient acetone, and embedded in Epon resin. The resin was then polymerized at 30 °C, 45 °C, and 60 °C for 1 day per temperature. The embedded tissues were cut at 70 nm, and the sections were stained with uranyl acetate and lead citrate. Transmission electron microscopy (FEI Tecnai G2 Spirit Bio TWIN, Japan) was used to observe the mitochondrial ultrastructure.

WGA staining and BODIPY staining

For WGA staining, the paraffin sections were dewaxed, rehydrated, and stained with 5 μg/mL WGA (Thermo Fisher Scientific, cat W11261, USA) in PBS for 30 min at room temperature. Then, the sections were washed with PBS 3 times and observed with a FluoView™ FV3000 confocal microscope (Olympus, Japan).

For BODIPY staining, the mouse hearts were fixed in 4% PFA, dehydrated in 30% sucrose, embedded in OCT and sectioned into 8 μm slices. Frozen sections were thawed at room temperature and stained with 2 μM BODIPY (Thermo Fisher Scientific, cat D3922, USA) for 15–20 min at 37 °C. Then, the sections were washed with PBS 3 times and observed with a FluoView™ FV3000 confocal microscope (Olympus, Japan).

Immunoblotting and immunoprecipitation

For western blot analysis, mouse hearts or cells were collected and homogenized with RIPA buffer containing protease inhibitor cocktails (Roche, cat 04693132001, Switzerland) and PMSF (1 mM). Then, the lysates were centrifuged at 12,000× g for 15 min at 4 °C. The supernatant was collected and boiled with loading buffer for 5 min.

The proteins were separated on an SDS-PAGE gel and transferred to a polyvinylidene difluoride (PVDF) membrane. Then, the membrane was blocked with 5% nonfat milk and incubated with the primary antibody overnight at 4 °C followed by washing with PBST. Then, the membrane was incubated with the indicated HRP-conjugated secondary antibody and detected with a chemiluminescent substrate.

For immunoprecipitation, the mouse hearts were collected and homogenized in IP buffer (25 mM Tris-HCl (pH 7.5), 150 mM NaCl, 2 mM EDTA, 1% Triton X-100, 5% glycerol) containing protease inhibitors cocktails and PMSF (1 mM). Then, the lysates were centrifuged at 12,000× g for 15 min at 4 °C. The supernatant was precleared with 1 µg of the appropriate control IgG and 20 µL of Protein A/G PLUS-Agarose (Santa Cruz, cat sc-2003, USA) and centrifuged at 1000× g for 5 min at 4 °C. Then, the supernatant was incubated with the indicated primary antibody overnight at 4 °C and incubated with Protein A/G PLUS-Agarose (Santa Cruz, cat sc-2003, USA) for 2–3 h at 4 °C. The immunoprecipitates were centrifuged, washed with IP buffer, and denatured. The proteins were analyzed by immunoblot. The primary antibodies used in the immunoblot analysis and immunoprecipitation are listed in Supplementary Table S5.

Mitochondrial isolation

Fresh mouse hearts were homogenized in mitochondrial isolation buffer (MIM, 70 mM sucrose, 210 mM mannitol, 5 mM HEPES, 1 mM EGTA, pH 7.2) using a drill-driven Teflon Dounce homogenizer. The homogenate was centrifuged at 800× g for 10 min at 4 °C. The supernatant was collected and centrifuged at 800× g for 10 min at 4 °C. Then the supernatant was collected and centrifuged at 8000× g for 10 min at 4 °C. The light layer was removed, and the pellet of isolated mitochondria was collected and washed three times with MIM. For Ac-coA or β-HB treatment, the mitochondria stored in MIM were treated with Ac-coA or β-HB for 2 h at 37 °C.

Measurement of OCR and ECAR

Cardiomyocytes were plated in XF24 Cell Culture Microplates before OCR or ECAR detection. Then, an XF24 Extracellular Flux Analyzer (Seahorse Bioscience, USA) was used for the analysis according to the manufacturer’s instructions. The OCR of isolated mitochondria was measured as described previously⁵². Extracellular flux analysis of the metabolic capacity utilizing fatty acids or glucose was performed using an XF Mito Fuel Flex Test Kit (Agilent, cat#103260-100) by detecting the OCR of a specific fuel when other fuel pathways are inhibited according to the manufacturer’s instructions.

Site-directed mutation of lysine and transfection

In IDH2 and SDHA protein domain, the lysine (K) to arginine (R) mutant plasmids were constructed. Overlap extension PCR with primers bearing mutations was performed to produce the point mutations in Idh2 and Sdha. All mutations were verified by DNA sequencing. The primer sequences are listed in Supplementary Table S4.

Measurement of the enzyme activity of IDH, SDH, and MDH

Mouse hearts were collected, and enzyme activity was detected immediately. The enzyme activities of IDH and SDH were measured using assay kits purchased from Solarbio (cat BC2160, cat BC0955, China) according to the manufacturer’s instructions. The enzyme activity of MDH was measured using assay kits purchased from Nanjing Jiancheng Bioengineering Institute (cat A021-2-1, China) according to the manufacturer’s instructions.

Statistical analysis

Quantitation was performed using Image J software. All data are presented as the means ± SEM of at least three independent experiments. The statistical significance of differences between two groups was analyzed by unpaired two-tailed Student’s t-test. Differences among multiple groups were analyzed by one-way ANOVA. P < 0.05 was considered to indicate statistical significance. *P < 0.05; **P < 0.01; ***P < 0.001.

Further details of materials and methods such as transcriptomic analysis and proteomic analysis are described in Supplementary Data S1.

Data availability

We submitted RNA sequencing data to the Gene Expression Omnibus under accession GSE207305 and GSE206797. The authors declare that all relevant data of this study are available within the article or from the corresponding author upon reasonable request.

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Acknowledgements

This work was supported by the Program of the National Natural Science Foundation of China (81830100), the National Key R& D Program of China (2018YFC1004703), and the Program of the National Natural Science Foundation of China (91857109, 31530046 and 31771492).

Author information

These authors contributed equally: Danyang Chong, Yayun Gu, Tongyu Zhang

Authors and Affiliations

Gusu School, State Key Laboratory of Reproductive Medicine and China International Joint Research Center on Environment and Human Health, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu, China
Danyang Chong, Yayun Gu, Yu Xu, Zhibin Hu & Chaojun Li
Model Animal Research Centre (MARC), Medical School of Nanjing University, National Resource Centre for Mutant Mice, Nanjing, Jiangsu, China
Danyang Chong, Tongyu Zhang, Dandan Bu, Zhong Chen, Na Xu, Haiquan Wang, Yangqing Li, Feng Zheng & Chaojun Li
State Key Laboratory of Membrane Biology and Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing, China
Liangkui Li, Peng Li & Li Xu
Department of Cardio-Thoracic Surgery, Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School, Nanjing, Jiangsu, China
Xiyu Zhu & Dongjin Wang

Authors

Danyang Chong
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Yayun Gu
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Tongyu Zhang
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Yu Xu
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Dandan Bu
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Zhong Chen
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Na Xu
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Liangkui Li
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Xiyu Zhu
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Haiquan Wang
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Yangqing Li
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Feng Zheng
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Dongjin Wang
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Peng Li
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Li Xu
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Zhibin Hu
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Chaojun Li
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Contributions

C.L. and D.C. designed the experiments and wrote the paper. D.C., Y.G., T.Z., Y.X, D.B, Z.C., L.L., X.Z., H.W., Y.L., and F.Z. performed experiments. N.X., D.W., P.L., L.X., Z.H., and C.L. provided materials and methodology support.

Corresponding authors

Correspondence to Li Xu, Zhibin Hu or Chaojun Li.

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The authors declare no competing interests.

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All animal procedures were performed under the guidelines of the institutional review board and the ethics committee of Nanjing Medical University.

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Table S2

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Table S5

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Chong, D., Gu, Y., Zhang, T. et al. Neonatal ketone body elevation regulates postnatal heart development by promoting cardiomyocyte mitochondrial maturation and metabolic reprogramming. Cell Discov 8, 106 (2022). https://doi.org/10.1038/s41421-022-00447-6

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Received: 22 December 2021
Accepted: 01 July 2022
Published: 11 October 2022
DOI: https://doi.org/10.1038/s41421-022-00447-6

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