Alveolar epithelial injury is one of the important pathological changes in idiopathic pulmonary interstitial fibrosis (IPF), but the regulatory mechanism remains unclear. Here, we reported that alveolar epithelial type-II cells (AT II) play important roles in pathological process of pulmonary fibrosis. Through iTRAQ (isobaric tagging for relative and absolute quantification) quantitative proteomics, TSSK4 was identified to be upregulated in bleomycin-induced fibrotic mice model, which was further confirmed in clinical IPF patients’ tissue specimens. TSSK4 is a germ-related protein, but its expression in other tissues and the association with other diseases are not reported. Immunofluorescence staining showed that TSSK4 selectively expressed in AT-II cells, which are essential for inflammation-induced AT-II loss during fibrosis. Luciferase assay and other molecular biological experiments proved that TSSK4 expression is regulated by TNF-α-mediated NF-κB signaling. The TSSK4 kinase activity is found to be closely related to the function of HSP90-AKT pathway that TSSK4 can phosphorylate its substrate HSP90β on serine 255, to inhibit the ATPase activity of HSP90β and reduce its molecular chaperone function on AKT. Under this condition, kinase activity of AKT is diminished to interfere its survival function, subsequently facilitating AT-II cellular apoptosis through the mitochondrial death machinery. Our findings highlight the importance of TSSK4 in regulating pulmonary fibrosis by facilitating AT-II loss through HSP90-AKT signaling, all of which suggest TSSK4 and the regulating mechanism as attractive targets for the clinical intervention of pulmonary injury and fibrosis.
Idiopathic pulmonary fibrosis (IPF) is a kind of severe respiratory disease with high lethality but limited treatment, and the etiology still remains unclear [1, 2]. The pathological features of IPF include excessive extracellular-matrix (ECM) deposition, honeycomb changes, interstitial scarring, and fibroblast foci, all of which ultimately lead to pulmonary-structure destruction and irreversible loss of lung function [3, 4]. The prevalence of IPF appears to be increasing [5,6,7]. It is imperative to further investigate the molecular regulatory mechanism of pulmonary epithelial injury and pulmonary fibrosis, as well as early intervention in therapeutic approaches.
Alveolar epithelial cells play an important role in maintaining the normal lung function . The pulmonary alveolar epithelium is mainly composed of two types of epithelial cells: alveolar type-I (AT I) and type-II (AT-II) cells. AT-II cells are considered to be progenitor (stem) cells of alveolar epithelium, which are responsible for epithelium regeneration after injury [9, 10]. It is reported that the pathological process of IPF involves repeated epithelial injury and abnormal damage repair, subsequently leading to the activation of intestinal fibroblasts, collagen deposition, and development of fibrotic lesions [11, 12]. It is reported that the function of AT II is impaired during pulmonary fibrosis [13, 14]. Nevertheless, it remains unclear how the AT-II dysregulation is triggered and what is the mechanism for AT II involving in IPF.
Testis-specific serine kinase 4 (TSSK4) is a member of the testis-specific serine kinases’ family. It is reported to mainly express in the acrosome part of sperm and participate in the formation of normal structure of sperm tail [15, 16]. Studies have shown that TSSK4 can induce apoptosis in some cell lines in vitro, such as HeLa, COS-7, and H1299 cell lines, and its pro-apoptotic function was verified to be related to its protein-kinase activity . Whether TSSK4 is expressed in other specific organizations to participate in other functions needs further verification.
AKT, also known as protein-kinase B (PKB), is involved in cell survival by inhibiting the apoptotic process . AKT could phosphorylate BAD (BCL2 antagonist of cell death), a pro-apoptotic protein of the BCL-2 family, to block the translocation of BAD to mitochondria and consequently interfere the mitochondrial apoptotic machinery . Heat-shock protein 90 (HSP90), including two isoforms α and β, is a widely existing and highly conserved molecular chaperone to assist its “client” proteins for stabilization and proper performance of protein functions [20, 21]. HSP90 protein is involved in many cellular behaviors, including cell cycle regulation, hormone-signal transduction, and cell homeostasis [22, 23]. HSP90 is reported to stabilize some signaling molecules, including PI3K and AKT proteins. Inhibition of HSP90 could restrict PI3K/AKT pathway, leading to downregulation of survival function and resulting in apoptosis of target cells .
In this article, based on high-throughput proteomic analysis, we report that TSSK4 is selectively expressed in AT II and promotes AT-II loss through HSP–AKT signaling axis during pulmonary fibrosis. It is one of the molecular mechanisms for the process of pulmonary fibrosis, in which interfering the expressing of TSSK4 or regulating the activity of AKT could be the potential therapeutic strategies to improve pathological process of IPF.
TSSK4 is upregulated in type-II alveolar epithelial cells in bleomycin-induced pulmonary fibrosis
To investigate the pathological progress of pulmonary fibrosis, we utilized a relatively mild-grade mice model that C57BL/6 mice were intratracheally challenged with 3 mg/kg body weight of bleomycin. General observation found that without obvious mortality, the body weight of the model mice decreased dramatically in the first 14 days after bleomycin induction, and gradually increased with the passage of time (Fig. S1A). Nevertheless, HE staining and Masson’s trichrome staining showed significant pulmonary tissue damage, collagen deposition and fibrotic response on days 14 and 21 (Fig. S1B, C), with significantly increased RNA level of fibrosis-related genes (Fig. S1D). Moreover, immunofluorescence staining showed the number of type-II alveolar epithelial cells (AT II, SP-C-positive staining) in the area of fibrotic lung tissue (type-I alveolar epithelial area with GP36-positive staining) observably decreased (Fig. 1A, B). In order to explore the key proteins that are dysregulated in the fibrotic lung tissue, lung tissues from bleomycin-induced mice models were analyzed by iTRAQ (isobaric tagging for relative and absolute quantification) quantitative proteomics (data are available via ProteomeXchange with identifier PXD027900). The results showed that in AT-II-associated proteins (LAMP3, ABCA3, and SFTPC), the expression of ABCA3 and SFTPC was significantly downregulated, which can also suggest the loss of AT II in pulmonary fibrosis (Fig. S1F). According to the analysis of iTRAQ, TSSK4 was detected to be upregulated (Fig. S1E, C). Based on the analysis of human protein Atlas database, it is worth noting that the RNA and protein of TSSK4 can also be detected in human lung tissues (Fig. S1G). The expression of TSSK4 in other tissues was also confirmed accordingly (Fig. S1H, I). We further confirmed the finding by immunoblotting and qPCR that although the protein and RNA level of TSSK4 were low in the resting condition, it significantly increased on days 14 and 21 of bleomycin induction, suggesting TSSK4 to be a kind of inducible protein in fibrotic lung tissues (Fig. 1D, E). It was further verified in the clinical human samples by immunohistochemistry analysis that compared with the paracancerous pulmonary tissues as control, the protein level of TSSK4 was significantly upregulated in clinical fibrotic lung tissues (in total, six fibrotic patient samples were tested with similar results, two of which are presented in Fig. 1F, G. Demographic information and the results of clinical examination are listed in Table 1). We further investigated the specificity of TSSK4 in lung tissue by immunofluorescence staining and the results showed that upregulated TSSK4 was mainly localized in AT-II cells (Fig. 1H), which strongly suggested that the upregulation of TSSK4 selectively occurred in AT-II cells.
TSSK4 upregulation is essential for bleomycin-induced pulmonary fibrosis
To investigate the contributions of TSSK4 upregulation in lung fibrosis, a vector of small-hairpin RNA targeting TSSK4 was imported into C57BL/6 mice by lentivirus-mediated intrapulmonary infection, to suppress TSSK4 expression in vivo (Fig. 2G, first panel). Pathological examination showed that compared with the control group, lung damage and collagen aggregation were significantly reduced in TSSK4-interference group (Figs. 2A and S2). It was also confirmed that TSSK4 knockdown distinctly reduced fibrotic responses, such as less induction of pulmonary fibrotic genes (Fig. 2B), less hydroxyproline in tissues (Fig. 2C), and statistically significant improvement of the symptoms of weight loss in fibrotic mice models (Fig. 2D). Immunohistochemistry and immunoblotting analysis showed that TSSK4 depletion could significantly reduce the loss of AT II in pulmonary tissue (Fig. 2E, G). Taken together, in fibrotic lung tissue, TSSK4 is selectively upregulated in AT-II cells, thereby promoting the loss of AT II and pathological progress of pulmonary fibrosis.
TSSK4 upregulation is regulated by NF-κB signaling pathway
To further investigate the upstream-regulatory mechanism of TSSK4 expression, we detected cytokines in pulmonary fibrotic mice tissues. The qPCR results showed that the mRNA level of TNF-α was significantly increased, but not TGF-β1 or TGF-β2 (Fig. 3A). To explore whether TNF-α was the key cytokine to promote TSSK4-selective expression in AT II, MLE12 (commonly used murine cell line of AT II) was performed to be stimulated with TNF-α (20 ng/ml). According to the stimulating time course, TSSK4 transcription gradually increased in MLE12, but not in MEFs (mouse embryonic fibroblasts) (Fig. 3B). Silencing of p65 in MLE12 inhibited TNF-α-induced TSSK4 expression both in protein and mRNA levels (Fig. 3C, D). It is consistent with the results of inhibitor treatment that NF-κB inhibitor (Bay 11–7082) but not P38 inhibitor (SB202190) could effectively block TSSK4 upregulation (Fig. S3A). All of these results suggest that TNF-α activated NF-κB to be the possible regulator of TSSK4 expression.
In order to further confirm the regulation of transcription factor p65 on TSSK4 gene expression, we analyzed the gene sequence of human TSSK4 (Gene ID: 283629) to identify the 5′-flanking region. Computational prediction indicated two potential p65-binding sites in the promoter region (about 1.8 kb up to transcript start site (TSS)) (Figs. 3E and S3B). As presented in the nucleotide schematic diagram, two truncated promoter fragments were constructed for dual-luciferase assay. The results showed that compared with full length of promoter region (−1.8 kb), the 5′- flanking region containing −0.8 kb is sufficient for maximal activity, suggesting it to be the major p65-bind region for TSSK4 gene transcription (Fig. 3F). The exact p65-binding nucleotide sequence requires further site mutation and report gene-activity analysis.
Taken together, our results indicate that TSSK4 expression is regulated by TNF-α-promoted NF-κB signaling pathway, and the p65-binding sites locate in the 5′-flanking region −0.8 kb of TSSK4-promoter region.
TSSK4 promotes AT-II loss through TNF-α-induced cellular apoptosis
Based on the results of quantitative proteomics (Fig. 1C), KEGG signaling pathway enrichment analysis revealed apoptosis-related signaling pathway to be activated (Fig. S4A). We hypothesized that the loss of AT-II cells in fibrosis might be through apoptosis. Immunofluorescence staining showed that according to the extension of bleomycin induction, the TUNEL-positive signals in lung sections gradually increased, which were mainly concentrated in AT-II cells (Figs. 4A, B and S4B). It was consistent with another immunofluorescence staining result that cleaved caspase-3 also concentrated on AT-II but not on AT-I cells (Fig. 4C). All of these results suggested apoptosis of AT-II cells during the pulmonary fibrotic process.
Caspase-3 activity assay showed that lentivirus-mediated shTSSK4 induction could significantly inhibit caspase-3 activity in fibrotic lung tissues (Fig. 4D). In vitro results showed that TNF-α could induce PARP cleavage in wild-type MLE12 cells (Fig. S4C), but less cleaved PARP was detected when TSSK4 expression was suppressed by shTSSK4 interfering (Fig. S4D and left part of Fig. 4E). On the contrary, TNF-α induced a more significant PARP cleavage in TSSK4-persistent expressing MLE12 cells (TSSK4+) than that in wild-type or TSSK4-deficient cells (Fig. 4E). Consistently, TNF-α could promote caspase activity and cellular apoptosis in TSSK4+ cells, which were both suppressed in TSSK4-deficient cells (Fig. 4F, G).
Taken together, TSSK4 upregulation in AT-II cells plays an important role in TNF-α-induced cellular apoptosis in vitro and AT-II loss in pulmonary fibrosis.
TSSK4 mediates AT-II apoptosis through the mitochondrial death machinery
It is reported that TNF-α cytotoxicity in diseases depends on the BH3-only protein BAD-mediated mitochondrial death machinery [19, 25]. In TSSK4-persistent expressing MLE12 cells (TSSK4+), immunofluorescence staining showed that TNF-α promoted the translocation of BAD protein to mitochondria (Figs. 5A, B and S5). Furthermore, mitochondrial membrane potential decreased significantly under TNF-α induction, which was totally suppressed in TSSK4-deficient MLE12 cells (Fig. 5C). Mitochondria/cytosol fractionation showed that in TSSK4+ cells, TNF-α promoted the percentage of BAD in the mitochondrial fractionation (Fig. 5D). Immunoprecipitation results also confirmed that TNF-α induced the interaction between BAD and mitochondrial outer-membrane protein BCL-xL in TSSK4+ cells but not in TSSK4-deficient cells (Fig. 5E). The same results further presented that BAD translocated to mitochondria in lung tissues of bleomycin-induced fibrosis, which was dramatically suppressed in lentivirus-mediated TSSK4-deficient model mice (Fig. 5F).
Taken together, AT-II apoptosis depends on the mitochondrial death machinery. Interfering TSSK4 expression could block the translocation of BAD to mitochondria, thereby inhibiting its pro-apoptotic function.
TSSK4 restrains AKT activation and consequently promotes AT-II apoptosis in vitro and in vivo
It was reported that PI3K–AKT pathway plays an essential role in cellular survival . IHC staining showed that compared with the control group, the level of phosphorylated AKT in bleomycin-induced fibrotic lung tissues was significantly upregulated when TSSK4 was depleted (Figs. 6A, B and S6A). TSSK4 depletion could significantly facilitate AKT phosphorylation under fibrotic condition (Fig. S6B, second panel). Meanwhile, immunofluorescence staining showed that AKT phosphorylation mainly occurred in AT-II cells (Fig. 6C). With the activation of AKT, serine 136 of BAD (the specific phosphorylation site by AKT to negatively regulate the pro-apoptotic function of BAD ) was also increased (Fig. S6B, first panel), but apoptotic signaling (PARP cleavage) was decreased (Fig. S6B, fifth panel). All of these data suggest AKT signaling pathway to be closely related to AT-II loss in pulmonary fibrosis.
As presented in (Fig. 6D), TNF-α stimulation induced upregulation of TSSK4 in MLE12 cells, but AKT phosphorylation was very weak. On the other hand, when blocking TSSK4 with shRNA, the AKT activation but not PI3K or p65 increased. It suggests that TSSK4 expression could suppress AKT activation in cells. As our results showed, TNF-α-induced apoptosis of TSSK4+ was significantly reduced when AKT was activated by AKT agonist SC-79 (Fig. 6E, F). On the other hand, in TSSK4-deficient cells, AKT inhibitor GSK690693 promoted TNF-α-induced apoptosis (Fig. S6C, D). Together, our data demonstrate that TSSK4 upregulation could inhibit AKT activation, thereby impairing its prosurvival function.
Because of weak AKT activation in bleomycin-induced pulmonary fibrosis (Fig. 6A, B), we further used AKT agonist SC-79 to treat mice. The results showed that intraperitoneal administration of SC-79 in the first 3 days of bleomycin induction could improve the fibrotic symptoms, including tissue pathological changes, collagen deposition, and body-weight loss (Figs. 6G–I and S6F). In brief, the AKT agonist contributes to enhance the prosurvival function of AKT, thereby ameliorating bleomycin-induced fibrosis.
TSSK4 phosphorylates HSP90β on serine 255
Since TSSK4 is a kind of serine-/threonine-phosphorylation kinase, we hypothesized that the mechanism of TSSK4-suppressing AKT activation was based on kinase-substrate reaction. In order to authenticate possible TSSK4-phosphorylation substrates and map out the precise phosphorylation site(s), lung tissues on day 14 of bleomycin induction were performed for iTRAQ quantitative-phosphorylation mass spectrometry. Phosphorylated HSP90β was detected with high detection ratio, and the phosphorylated site was serine 255 as the mass spectrogram presented (Fig. 7A). To further confirm HSP90β as the substrate of TSSK4, we replaced the predicted serine 255 with nonphosphorylated alanine using a site-directed mutagenesis approach. TSSK4 immune-complex kinase assay showed that TNF-α stimulation could promote TSSK4 to phosphorylate the GST-HSP90β (WT), but not GST-HSP90β (S255A) mutant (Fig. 7B). Together, HSP90β is the substrate of TSSK4 with serine 255 as the specific phosphorylation site.
TSSK4 phosphorylates HSP90β to suppress HSP90–AKT signaling axis for AT-II apoptosis
GST pull-down experiments showed that TNF-α induced mild interaction between AKT and wild-type GST-HSP90β (Fig. 7C, left panels). This kind of AKT-HSP90β interaction was substantially increased when serine 255 of HSP90β mutated with nonphosphorylate alanine (S255A) (Fig. 7C, middle panels). On the contrary, AKT-HSP90β interaction was dramatically reduced when serine 255 mutated with phosphorylated mimic aspartic acid (S255D) (Fig. 7C, right panels). The results indicate that TSSK4 phosphorylating HSP90β on serine 255 could interfere its interaction with AKT. It was further proved by the immunoprecipitation results that TNF-α induced a more significant AKT-HSP90β interaction in TSSK4-deficient cells than that in TSSK4-persistent expressing MLE12 cells (TSSK4+) (Fig. 7D).
Because the chaperone function of HSP90β is in ATP-dependent manner, we next detected the effect of TSSK4 on the ATPase activity of HSP90. Our results showed that TNF-α could promote ATPase activity of HSP90β in TSSK4-deficient MLE12 cells, but no effect in TSSK4+ cells (Fig. 7E). Immunoblotting results showed that TNF-α could promote AKT phosphorylation and activation, consequently less PARP cleavage in TSSK4-deficient cells, all of which were totally reversed when cells were pretreated with HSP90 inhibitor 17-AAG (Fig. 7F). It was consistent with cellular apoptotic analysis that 17-AGG dramatically enhanced TNF-α-induced apoptosis in TSSK4-deficient MLE12 cells (Fig. 7G).
Taken together, TSSK4 expression could suppress the ATPase activity of HSP90, interfered its interaction with AKT, and thereby affects the activation of AKT and its prosurvival function.
In this report, we demonstrate that TNF-α induces selective expression of TSSK4 in AT II cells, thereby facilitates the apoptotic pathway for AT II loss, local lung tissue damage and fibrosis as pathological characters. At the molecular level, TNF-α-mediated NF-κB signaling pathway initiates TSSK4 gene expression in AT II. TSSK4 can phosphorylate its substrate HSP90β on serine 255, to inhibit its ATPase activity and reduce its molecular chaperone function on AKT. Under this condition, kinase activity of AKT is diminished to interfere its survival function, that pro-apoptotic protein BAD, without its negative regulatory phosphorylation by AKT (serine 136), translocates to the outer membrane of mitochondria to promote apoptosis through the mitochondrial death machinery (Fig. 8).
The in situ staining and other cellular results further demonstrated that AT-II loss depends on mitochondrial apoptotic machinery (Figs. 4A, C and 5A). The qPCR results showed that the mRNA level of TNF-α was significantly increased (Fig. 3A), which suggests TNF-α to be the major trigger of AT-II apoptosis. According to literatures, besides TNF-α, TGF-β is also important for the development of IPF. TGF-β superfamily includes TGF-β1, TGF-β2, and bone morphogenic proteins (BMPs). It is reported that the crosstalk between TGF-β and BMPs plays important roles in fibrotic processes. In lung, BMP-4 and BMP-7 could play antergic function against TGF-β to improve fibrosis [28, 29]. Based on our results (Fig. 3A), various expression levels of TGF-β superfamily (TGF-β 1, TGF-β 2, BMP-2, and BMP-4) were detected, suggesting the complexity of TGF-β superfamily in fibrosis. Further research is required to investigate the feedback regulation of TGF-β superfamily and the crosstalk among different members for fibrotic progress.
TSSK4-mediated HSP90 suppression leads to AKT deactivation in AT II, which is one of the important mechanisms for AT-II loss in fibrosis (Fig. 8). AKT plays an important role in lots of cellular behaviors, such as cell survival, cell differentiation, cellular activation, etc., but the contribution of AKT pathway in fibrosis is complicated and remains to be further investigated. For lung fibrosis, AKT activation is reported in different cells with different pathological functions. For example, AKT activation in macrophages could promote several fibrotic factors for wound healing and organ fibrosis; AKT activation in fibroblasts could regulate collage expression . From these perspectives, AKT inhibitors could be as potential therapeutic agents for IPF . On the other hand, we found that AKT was suppressed selectively in AT-II cells because of TSSK4 expression that promoted AT-II loss under inflammatory conditions. It is convinced that AT-II loss happens at the early stage of IPF and further promotes pulmonary damage and subsequent fibrosis. Therefore, it is meaningful for AKT agonist application in the early stage of IPF to prevent AT-II loss. It is not contradicted with AKT-inhibitor application mentioned above, because both of them focus on different molecular mechanisms and targets in different pathological stages during the whole IPF progress. Our data showed that in vivo application of AKT agonist SC-79 has an obvious inhibitory effect on bleomycin-induced pulmonary fibrosis (Fig. 6G, I). According to our results, SC-79 treatment in the first 3 days has a significant effect on fibrosis inhibition (Fig. 6G, I), but the intervention effect is not obvious or even counterproductive if treated with SC-79 for more than 7 days (data not shown). We believe that the effect of SC-79 is focused on the apoptosis of AT II as the inhibitory target in the initial stage. The key point is to find more specific compounds/drugs to target on the core regulatory cells and processes. AT-II loss and pulmonary damage are the initiator of fibrosis but fibroblast activation involves in the subsequent pathological progress. AT-II protection and fibroblast suppression are two opposite and independent potential strategies for clinical treatments of IPF. Further in-depth research is required to investigate the efficacy of early application of AKT agonist (such as SC-79) combined with late-selective fibroblast suppression in pulmonary fibrosis therapeutic treatment.
Taken together, given the importance of TSSK4 upregulation in AT II for cellular apoptosis and thereby fibrosis, our finding is likely to have great significance for further understanding the pathogenesis of pulmonary fibrosis and subsequent clinical application of IPF.
Materials and methods
Antibodies against TSSK4 (A7861) and caspase-3 (A0214) were purchased from ABclonal. Antibodies against HSP90β (GB12284) and PARP (GB111500) were from Servicebio. Antibody against Phospho-PI3-kinase p85/p55 (YP0224) was from ImmunoWay. Antibodies against SP-C (10774-1-AP), Tom20 (11802-1-ap), and p-AKT (66444-1-ig) were from Proteintech. Antibodies against p-BAD Ser136 (9295), BCL-xL (2762), and β-actin (c3700) were from CST. Antibodies against phosphoserine (ab9332), GP36 (ab11936), and p65 (ab16502) were from Abcam. Compounds SC-79 (T2274), Bay11-7082 (19524-67-7), SB202190 (152121-30-7), and 17-AAG (T6290) were from Target Mol. TNF-α (murine) was from R&D. JC-1 Assay Kit was from Molecular Probes of Life Technologies. Hydroxyproline (HYP) Assay Kit (20201102) was from Solarbio.
For TSSK4 gene silence, small-hairpin RNA targeting TSSK4 was constructed in pLV[shRNA]-EGFP: T2A: Puro vector. Three different target sequences are listed as:
shTSSK4-1: CCACGTGAGATACAGGTAATG; shTSSK4-2: CATCGTGCACCGGGATTTAAA; shTSSK4-3: CTCGAATGGATCCAACGATAT.
For TSSK4 expression, mTSSK4-encoding cDNA was subcloned in pLV[Exp]-EGFP: T2A: Puro vector. The recombinant lentivirus-encoding targets or invalid vectors were generated using standard procedures and purified by glucose density-gradient centrifugation.
The sequence of sip65-1: GAGUUUCAGCAGCUCCUGAAC, and siCtrl: GGAGCGCACCAUCUUCUUC, was synthesized from Tsingke. sip65-2 siRNA (6339) was directly purchased from CST.
Expression vector encoding GST-HSP90β was subcloned in pET-GST/TEV vector. S255A and S255D mutant were introduced using Mut Express II fast mutagenesis kit (Vazyme) and verified by DNA sequencing.
Cell culture and stable cell lines’ establishment
MLE-12 was purchased from ATCC and cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum, 2 mM glutamine, 100 U/ml penicillin, and 100 mg/ml streptomycin, at 37 °C with 5% CO2. All of the cells in experiments have been tested to be mycoplasma free.
To establish TSSK4−/− stable cell line, MLE12 cells were transfected with pLV[shTSSK4]-EGFP: T2A: Puro plasmid and selected with puromycin (1.5 μg/ml).
For TSSK4-persistent expressing MLE12 cells (TSSK4+), TSSK4−/− stable cells were transiently infected with lentivirus-mediated pLV[TSSK4]-EGFP-expression vector for 48 h. The results of regulated TSSK4 expression were tested by immunoblotting.
C57BL/6 mice were purchased from SPF Biotechnology Co., LTD (SCXK 2016-0002, China). Mice were maintained under controlled conditions (indoor temperature: 22 ± 1 °C and humidity: 40–60%) and a 12 h dark–light cycle in the specific pathogen-free (SPF) facility. Male 6–8-week-old mice were used in this study. All animal procedures were conducted in accordance with the protocols approved by the research ethics committee of the Second Affiliated Hospital of Guangzhou Medical University.
For bleomycin-induced pulmonary fibrotic mice model, C57BL/6 male mice were treated with 3 mg/kg bleomycin or the same amount of sterile saline via intratracheal administration.
For TSSK4 knockdown in vivo, C57BL/6 male mice were intratracheally infected with lentivirus-mediated shTSSK4 or control vectors at the dose of 1.05 × 1010 infectious units (IFUs) in a volume of 30 μl per animal, followed with bleomycin-induced pulmonary fibrosis.
For AKT-agonist administration in vivo, bleomycin-induced pulmonary fibrotic mice were daily intraperitoneally injected with SC-79 (40 mg/kg body weight in 10% DMSO) or the same amount of DMSO in the first 4 days.
Body weight was detected every two days. The mice were preeuthanatized on days 7, 14, and 21 after bleomycin induction. Lung tissues were collected and stocked at −80 °C for molecular analysis, including immunoblotting and hydroxyproline assay, according to the manufacturer’s protocol. For pathological analysis, lung lobes were fixed in 4% paraformaldehyde for 12 h. The tissues were sliced to 5 μm thickness for hematoxylin and eosin (HE) staining, Masson’s trichrome staining, and immunohistochemical or immunofluorescence staining, according to the manufacturer’s protocols.
Clinical IPF-tissue specimens
Lung-tissue slices from IPF patients were obtained from the Biological Sample Bank of State Key Laboratory of Respiratory Diseases and pathological department of the Second Affiliated Hospital of Guangzhou Medical University. The project was approved by the ethics committee of the Second Affiliated Hospital of Guangzhou Medical University.
Subcellular fractionation and caspase-3 activity assay
To detect subcellular localization of BAD proteins, cells or tissue lysis were performed for cytosol/mitochondrial fractionation with kit (Abcam). Fractions from the same groups were analyzed by immunoblotting. The sum of BAD proteins in different fractions was set as 100%, and the ratio of BAD in different subcellular fractions was analyzed by ImageJ program.
For caspase-3 activity assay, total lysis from cells or tissues was performed with kit using Ac-DEVD-pNA as substrate (Calbiochem).
Apoptosis assay, mitochondrial membrane potential, and quantitative real-time PCR
For apoptosis analysis, cells were harvested by Trypsin digestion after different treatments as indicated in figure legends. Annexin V/propidium iodide (PI) staining was performed with Annexin V-AF647/PI apoptosis kit (ES science), following with flow cytometric analysis (BD Biosciences).
For mitochondrial membrane potential, the JC-1 Assay Kits were used to stain cells according to the manufacturer’s instruction. The mitochondrial membrane potential was indicated by a fluorescence-emission shift from monomer to polymer and the ratio of mean fluorescence intensity (MFI) was analyzed by flow cytometer.
For quantitative real-time PCR analysis, total RNA was extracted by Trizol reagent from cells or tissues. Total RNA (1 μg) was used for cDNA synthesis, following quantitative PCR analysis with SYBR Green real-time PCR master Mix (Invitrogen). PCR primers were synthesized from Tsingke. GAPDH was used as internal control. The sequences of primers were listed as below:
TSSK4: sense, 5′-CGCCAATCTACCAAGCGTG-3′; antisense, 5′-TGCCGTTTAGCAGAGCTGG-3′
TGF-β1: sense, 5′-CTCGGGGGCTGCGGCTACTG-3′; antisense, 5′- GGCGTATCAGTGGGGGTCA -3′
TGF-β2: sense, 5′-CGAGCGGAGCGAGCAGGAG-3′; antisense, 5′-TAGGAGGGCAACAACATTA-3′
BMP2: sense, 5′-GGGACCCGCTGTCTTCTAGT-3′; antisense, 5′- TCAACTCAAATTCGCTGAGGAC-3′
BMP4: sense, 5′-GACTTCGAGGCGACACTTCTA-3′; antisense, 5′-GCCGGTAAAGATCCCTCATGTAA-3′
mTNF-α: sense, 5′-TCTTCTCATTCCTGCTTGTGG-3′; antisense, 5′-GGTCTGGGCCATAGAACTGA-3′
Timp1: sense, 5′-GCAAAGAGCTTTCTCAAAGACC-3′; antisense, 5′-AGGGATAGATAAACAGGGAAACACT-3′
Col3: sense, 5′-AACCTGGTTTCTTCTCACCCTTC-3′; antisense, 5′-ACTCATAGGACTGACCAAGGTGG-3′
Mmp12: sense, 5′-CTGGGCAACTGGACAACTCAACTC-3′; antisense, 5′-AATGCTGCAGCCCCAAGGAAT-3′
α-SMA: sense, 5′-CCCCTGAAGAGCATCGGACA-3′; antisense, 5′-TGGCGGGGACATTGAAGGT-3′
GST protein pulldown, immunoprecipitation (IP), HSP90β ATPase activity assay, and kinase assay
For GST pulldown, GST-tagged HSP90β proteins (WT, S255A, or S255D mutant) were IPTG (0.1 mM) induced and purified from BL21 bacteria containing prokaryotic expression vectors of GST fusion target proteins. Incubated with different cellular lysis as indicted in figure legends, GST proteins together with binding targets were precipitated by GSH-Sepharose beads and detected by immunoblotting. The amount of GST-tagged proteins in the system was detected by Coomassie brilliant blue staining.
IP assay was performed as previously described25. In brief, 100 μg of total cellular lysates were mixed with 30 μL of protein-A Sepharose beads (50% slurry) containing 4 μl of anti-TSSK4 antibody, and rotated at 4 °C overnight. After short centrifuge, pellet complexes were collected to detect the binding proteins by immunoblotting.
For HSP90β ATPase activity assay, HSP90β in cells was immunoprecipitated with anti-HSP90β antibody. The ATPase activity was detected with total ATPase Assay Kit (Nanjing Jiancheng Biotech., A070-1) according to the manufacturer’s protocols.
For TSSK4 kinase assay, TSSK4 proteins were immunoprecipitated from TSSK4+ cells without or with TNF-α treatment as indicated in figure legends. Purified GST-HSP90β proteins (WT or S255A mutant) were used as substrates to incubate with precipitated TSSK4 in the presence of 0.2 mM cold ATP (nonradioactive) in 30 °C for 1 h. Substrate phosphorylation was detected by immunoblotting with antiphosphoserine antibody.
To examine the p65 transcript activity, different fragments of TSSK4 promoters were subcloned in pGL3 vector containing the firefly luciferase reporter gene. The constructed pGL3-promoter plasmids along with pCDNA3.1–p65 expression vector and Renilla luciferase reporter vector were cotransfected in 293T cells. At 48 h after transfection, firefly and Renilla luciferase activities were detected by the Dual-Light Combined Reporter Gene Assay System (Promega). The RLU (relative light unit) was normalized by the Renilla reporter signaling.
Cellular apoptosis assay, qPCR, hydroxyproline assay, and luciferase assay between two groups were presented as mean ± s.d. Statistical analysis was conducted using two-tailed unpaired Student’s t test. For immune-staining data, at least five random fields were picked for ImageJ program analysis, and conducted using two-tailed unpaired Student’s t test. For body-weight-loss analysis, the results were interpreted using one-way ANOVA test. For animal experiments, mice with same age and similar body weight were blinded and randomly divided into different groups. At least 5 mice per group to ensure adequate power, and all of the animal data were included for analysis. Similar variation of each group/treatments was estimated before statistical analysis. In all experiments, p < 0.05 was considered to be significant.
All data associated with this study are presented in the paper or the Supplementary Materials. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD027900.
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This study was funded by the Key Scientific Research Projects of University in Guangdong Province (2018KZDXM057), University Scientific Research Project of Guangzhou Education Bureau (202032805), and Health Science and Technology Project of Guangzhou City (20211A010057). We are also grateful for the support from 111 Project (D18010).
The authors declare no competing interests.
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Chen, H., He, A., Li, H. et al. TSSK4 upregulation in alveolar epithelial type-II cells facilitates pulmonary fibrosis through HSP90-AKT signaling restriction and AT-II apoptosis. Cell Death Dis 12, 938 (2021). https://doi.org/10.1038/s41419-021-04232-3