Correction to: Cell Death & Disease
https://doi.org/10.1038/s41419-020-2545-6 published online 5 May 2020
In addition, the last sentence of the “Material and methods” section describing the Detection of exosomes uptake by cardiomyocytes contained incorrect information on labelling. The full and corrected paragraph is provided below.
Isolated exosomes were incubated with 3.3 μL of Alexa FlourTM 488 C5 Maleimide (200 μg/mL, A10254, Thermo Scientific, San Jose, CA, USA) for 1 h at room temperature. The labelling was disturbed by passing through the exosome spin column (MW3000, 4484449, Thermo Scientific, San Jose, CA, USA), according to manufacturer’s instruction. The labelled exosomes were washed out and resuspended with 1 mL of serum free OptiMEM (31985088, Thermo Scientific, San Jose, CA, USA). For each well in a 4-well plate, 250 μL labelled exosomes were incubated with primary cardiomyocytes in the standard cell culture condition for 4 h at 37 °C. Cardiomyocytes were then counterstained with CellTracker Deep Red dye and mounted with ProLong Gold antifade mountants without DAPI (#P36934, Thermo Scientific, San Jose, CA, USA). The cells labelling with Alexa 488 (green) under the microscope were considered as positive cells containing exosomes.
The authors apologise for these errors.
Edited by G. Melino
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Peng, Y., Zhao, JL., Peng, ZY. et al. Correction: Exosomal miR-25-3p from mesenchymal stem cells alleviates myocardial infarction by targeting pro-apoptotic proteins and EZH2. Cell Death Dis 11, 845 (2020). https://doi.org/10.1038/s41419-020-03025-4