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Cellular and Molecular Biology

A multimodal imaging study to highlight elastin-derived peptide pro-tumoral effect in a pancreatic xenograft model

British Journal of Cancer volume 128, pages 2000–2012 (2023)Cite this article

Subjects

Abstract

Background

Pancreatic ductal adenocarcinoma (PDAC) is highly malignant with a very poor prognosis due to its silent development and metastatic profile with a 5-year survival rate below 10%. PDAC is characterised by an abundant desmoplastic stroma modulation that influences cancer development by extracellular matrix/cell interactions. Elastin is a key element of the extracellular matrix. Elastin degradation products (EDPs) regulate numerous biological processes such as cell proliferation, migration and invasion. The aim of the present study was to characterise for the first time the effect of two EDPs with consensus sequences “GxxPG” and “GxPGxGxG” (VG-6 and AG-9) on PDAC development. The ribosomal protein SA (RPSA) has been discovered recently, acting as a new receptor of EDPs on the surface of tumour cells, contributing to poor prognosis.

Methods

Six week-old female Swiss nude nu/nu (Nu(Ico)-Foxn1nu) mice were subcutaneously injected with human PDAC MIA PaCa-2/eGFP-FLuc+ cells, transduced with a purpose-made lentiviral vector, encoding green fluorescent protein (GFP) and Photinus pyralis (firefly) luciferase (FLuc). Animals were treated three times per week with AG-9 (n = 4), VG-6 (n = 5) or PBS (n = 5). The influence of EDP on PDAC was examined by multimodal imaging (bioluminescence imaging (BLI), fluorescence imaging (FLI) and magnetic resonance imaging (MRI). Tumour volumes were also measured using a caliper. Finally, immunohistology was performed at the end of the in vivo study.

Results

After in vitro validation of MIA PaCa-2 cells by optical imaging, we demonstrated that EDPs exacerbate tumour growth in the PDAC mouse model. While VG-6 stimulated tumour growth to some extent, AG-9 had greater impact on tumour growth. We showed that the expression of the RPSA correlates with a possible effect of EDPs in the PDAC model. Multimodal imaging allowed for longitudinal in vivo follow-up of tumour development. In all groups, we showed mature vessels ending in close vicinity of the tumour, except for the AG-9 group where mature vessels are penetrating the tumour reflecting an increase of vascularisation.

Conclusions

Our results suggest that AG-9 strongly increases PDAC progression through an increase in tumour vascularisation.

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Fig. 1: RPSA expression in healthy and pancreatic tissue.
Fig. 2: Generation of stable reporter gene expression in the pancreatic cancer cell line for optical imaging.
Fig. 3: Evaluation of the elastin-derived peptide on tumour growth in a pancreatic mouse model.
Fig. 4: Optical imaging approach enables in vivo assessment of the effect of elastin-derived peptide on tumour growth.
Fig. 5: Magnetic resonance imaging (MRI) based in vivo assessment of the effect of elastin-derived peptide on tumour growth.
Fig. 6: 3D MRI angiography for in vivo assessment of the effect of elastin-derived peptide on tumour vascularisation and tumour growth.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

We gratefully acknowledge the VIB Bio Imaging Core of KU Leuven for their support and assistance in this work. We thank the PICT-IBiSA Platform of the University of Reims Champagne-Ardenne (France), Dr. Carla Rios Luci and Pr. Stefaan Soenen (NanoHealth and Optical Imaging Group, KU Leuven, Belgium), Dr. Bella Manshian (Translational Cell and Tissue Research, KU Leuven, Belgium) and Aurélie Dupont-Deshorgue (UMR7369) for their skilful technical assistance.

Funding

This work was supported by grants from the European Commission for the PANA project (H2020-NMP-2015-two-stage, grant 686009), the Centre National de la Recherche Scientifique (UMR7369), the University of Reims Champagne-Ardenne, the Region Champagne-Ardenne and the Department Imaging and Pathology of KU Leuven provided a FLOV mandate to partially finance LNs PhD scholarship.

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  1. These authors contributed equally: Uwe Himmelreich, Bertrand Brassart.

Authors and Affiliations

  1. KU Leuven, Department of Imaging and Pathology/Biomedical MRI, Leuven, Belgium

    Lise Nannan, Willy Gsell, Sarah Belderbos, Jens Wouters & Uwe Himmelreich

  2. CNRS UMR 7369 Matrice Extracellulaire et Dynamique Cellulaire, Reims, France

    Lise Nannan, Célia Gallet, Sylvie Brassart-Pasco & Bertrand Brassart

  3. Université de Reims Champagne Ardenne, Laboratoire de Biochimie Médicale et Biologie Moléculaire, Reims, France

    Lise Nannan, Célia Gallet, Sylvie Brassart-Pasco & Bertrand Brassart

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Contributions

LN, WG, UH, CG, JW, SB and BB designed, performed and analysed all the experiments. LN wrote the paper with BB, WG, UH and SBP provided essential editorial oversight and critical review. BB and UH jointly supervised this work.

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Correspondence to Bertrand Brassart.

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Nannan, L., Gsell, W., Belderbos, S. et al. A multimodal imaging study to highlight elastin-derived peptide pro-tumoral effect in a pancreatic xenograft model. Br J Cancer 128, 2000–2012 (2023). https://doi.org/10.1038/s41416-023-02242-w

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