Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

Clinical Study

First-in-human Phase 1 open label study of the BET inhibitor ODM-207 in patients with selected solid tumours

Abstract

Background

Bromodomain and extra-terminal domain (BET) proteins are reported to be epigenetic anti-cancer drug targets. This first-in-human study evaluated the safety, pharmacokinetics and preliminary anti-tumour activity of the BET inhibitor ODM-207 in patients with selected solid tumours.

Methods

This was an open-label Phase 1 study comprised of a dose escalation part, and evaluation of the effect of food on pharmacokinetics. ODM-207 was administered orally once daily. The dose escalation part was initiated with a dose titration in the initial cohort, followed by a 3 + 3 design.

Results

Thirty-five patients were treated with ODM-207, of whom 12 (34%) had castrate-resistant prostate cancer. One dose-limiting toxicity of intolerable fatigue was observed. The highest studied dose achieved was 2 mg/kg due to cumulative toxicity observed beyond the dose-limiting toxicity (DLT) treatment window. Common AEs included thrombocytopenia, asthenia, nausea, anorexia, diarrhoea, fatigue, and vomiting. Platelet count decreased proportionally to exposure with rapid recovery upon treatment discontinuation. No partial or complete responses were observed.

Conclusions

ODM-207 shows increasing exposure in dose escalation and was safe at doses up to 2 mg/kg but had a narrow therapeutic window.

Clinical trial registration

The clinical trial registration number is NCT03035591.

Background

Acetylation of lysine residues on histone tails is associated with open chromatin and transcriptional activation.1 Bromodomains are a highly evolutionarily conserved family of proteins responsible for binding acetylated lysine residues at the amino-terminal tails of histones.2 The bromodomain and extra-terminal (BET) family (BRD2, BRD3, BRD4 and BRDT) contain two tandem bromodomains which bind acetylated lysine residues, facilitating recruitment of transcriptional proteins to chromatin.3 BRD4 localises to promoter and enhancer regions of chromatin, and functions to regulate RNA-pol II-mediated elongation and transcription through interactions with the Mediator complex and pTEFb.4,5 In many tumour types, the efficacy of BET inhibitors has been attributed to the transcriptional suppression of genes such as MYC.6 More significantly, evidence of the oncogenic nature of BRD4 is apparent in the rare and aggressive tumour known as nut midline carcinoma (NMC), which occurs as the result of a chromosomal translocation with a resultant fusion protein of BRD4 with nuclear protein in testis (NUT).7 Although less common, the BRD3-NUT fusion protein may confer a better prognosis whilst still potentially responding to therapeutic inhibition with BET inhibitors.8,9 Importantly, there is evidence that preclinical activity of various BET inhibitors in various tumour types can be both MYC dependent6 and MYC independent.10 Consequently, the BET family of proteins are a promising candidate for anti-cancer therapy.

Several small molecule BET inhibitors, predominantly structurally related to JQ1, are in clinical development and have shown some preliminary clinical activity in NMC and lymphoma.11,12,13,14 These studies showed BET inhibition strongly correlated with decreasing platelet count.13,14 ODM-207, a novel small molecule that is structurally unrelated to JQ1, is a highly potent pan-BET inhibitor that has shown preclinical evidence of tumour growth inhibition in breast cancer and in leukaemia and prostate cancer xenograft models.15,16,17,18 ODM-207 has shown minimal cross-reactivity with non-BET family bromodomains.

Here, we describe the first-in-human Phase 1 study designed to investigate the safety, pharmacokinetics and maximum tolerated dose (MTD) of ODM-207 in patients with advanced malignancies. Platelet count was chosen as a pharmacodynamic biomarker based on the observed robust correlation between BET inhibitor exposure and thrombocytopaenia in preclinical and clinical studies.13,14

Methods

Study design

This was a Phase 1–2, multicentre, non-randomised, open-label, dose-escalation study of ODM-207 (NCT03035591, Orion Corporation) in patients with selected solid tumours. The primary objectives were to determine the safety and tolerability, dose-limiting toxicities (DLT), and maximum tolerated dose (MTD) of ODM-207. The secondary objectives of the study were to characterise the pharmacokinetics of ODM-207 and its main metabolite, to evaluate the effects of food on bioavailability and to generate preliminary evidence of target inhibition and anti-tumour activity.

The study design contained two parts: (1) dose escalation in patients with advanced solid tumours with an innovative single dose accelerated titration procedure in the first cohort (1A), (2) evaluation of food effect on bioavailability (1B). The single-dose accelerated titration procedure in the first cohort involved comparison of measured pharmacokinetic parameters in the first cohort of patients against preclinical models to evaluate whether exposure was within the target range. If the measured exposure was outside the target range (below or above), the protocol allowed for dose adjustment. Dose escalation was performed using a traditional 3 + 3 schedule. The food effect cohort involved a single fasted dose (overnight fasting, food allowed 4-h after dosing); after a washout period ODM-207 was given after a light breakfast (200–300 kcal of which 50–100 kcal from fat). The relevant national authorities provided regulatory approval and local institutional review boards approved the protocol. All study procedures were conducted in accordance with the Declaration of Helsinki and good clinical practice. All patients provided written informed consent.

Patients and drug administration

Patients in the dose escalation part received oral ODM-207 once daily in a 28-day cycle at a starting dose of a 50 mg tablet daily under fed conditions. The starting dose was chosen as it was reasonably expected to be both pharmacologically active and safe and tolerable. The target exposure for the starting dose was estimated from exposures observed in pre-clinical anti-tumour activity and safety studies. The estimated human dose required was predicted by both human equivalent dose calculations and physiologically-based pharmacokinetic modelling. A single dose titration procedure was performed in the first cohort of patients to confirm that exposure of ODM-207 in humans corresponded with the pre-defined target exposure based on non-clinical data. The starting dose provided plasma exposure that was in the target range. Therefore, no further dose titration in the first cohort was pursued (Fig. S1). Treatment was taken in the ambulatory setting, except for hospital admissions required for pre-specified study-related procedures.

Eligible patients had histologically confirmed NMC, high-grade serous ovarian cancer, HER2 negative breast cancer, castrate-resistant prostate cancer, small cell lung cancer, non-small cell lung cancer, melanoma, non-Hodgkin lymphoma, sarcoma or any other tumour predicted to have a significantly higher likelihood of response to ODM-207 (such as MYC amplified tumours), with no effective standard therapy available. Patients had to have ECOG performance status ≤ 1, adequate haematopoietic, renal, hepatic and coagulation function, life expectancy >12 weeks and needed a washout period from prior anti-cancer therapy.

Procedures and safety assessments

Safety was assessed at baseline, continuously during treatment and for 28 days following cessation of ODM-207. The National Cancer Institute Common Terminology Criteria for Adverse Events (version 4.03) were used to grade treatment-emergent adverse events (AEs).19 DLTs, defined as AEs related to ODM-207 that occurred within the first 28 days (cycle 1), included grade 4 neutropaenia lasting more than seven days, febrile neutropaenia, grade 3 thrombocytopaenia with grade 3 bleeding or grade 4 thrombocytopaenia lasting more than three days, treatment interruption of 14 days, persistent grade 3 gastrointestinal toxicity despite optimal medical intervention, grade 3 liver function abnormalities lasting more than seven days. The MTD was defined at the highest dose at which <33% of patients experienced DLTs during cycle 1 within a cohort. Response was assessed using RECIST 1.1 for patients with advanced solid tumours,20 Prostate Cancer Working Group 3 criteria (PCWG3) for castrate-resistant prostate cancer21 and Lugano criteria for non-Hodgkin lymphoma.22 Blood samples were taken at nine time points on initial dosing, after two weeks of dosing and before dosing in all follow up visits to establish the pharmacokinetic profile (maximum concentration, maximum time, area under the plasma concentration-time curve, volume of distribution, clearance and half-life, and minimum concentration at long term steady state) of ODM-207.

Statistical analyses

Safety analyses included all patients who received any doses of treatment. Efficacy evaluation was performed on all patients who received two or more cycles of treatment (≥8 weeks). Data points were excluded from relevant pharmacokinetic and platelet count analyses if samples were taken when the patient was on treatment interruption or within 3 days after resumption of treatment. Three levels of exposure (low, mid, high) were defined based on pre-clinical pharmacological in-house studies about ODM-207 and observed effects of ODM-207 on count of platelets. Pre-dose total exposure of <3500 ng/mL was not expected to have significant BET inhibition effect and a level of ≥5000 ng/mL was expected to confer BET inhibition.

Results

Between 22 December 2016 and 10 May 2019, 36 patients were enrolled, and 35 patients were treated at doses ranging from 50 mg to 205 mg corresponding with a range of 0.6–2.0 mg/kg. After a dose-limiting toxicity of fatigue was observed in one subject in cohort 1A-2 (100 mg), a decision was made to change the dosing regimen to weight-based dosing due to a wide range of exposure and assumed narrow therapeutic range. Dose escalation continued with mg/kg-based dosing up to the level of 2 mg/kg. This dose level was determined to be the MTD based upon observed adverse events, although further DLTs were not observed. The demographic information is shown in Table 1. Of the 35 treated patients, 12 (34%) had castrate-resistant prostate cancer (CRPC), 5 (14%) had melanoma, 4 (11%) had NMC, 4 (11%) had oestrogen receptor positive breast cancer (ER + BC) and 10 (29%) had other tumour types. Median age was 60 years (range 17–78 years). Treatment discontinuation occurred due to disease progression in 28 (80%) patients, due to AEs in 5 (14%) patients and 2 (6%) for other reasons.

Table 1 Patient demographics.

Dose escalation

O 36 patients enrolled in the study, 35 were evaluable for safety (one patient did not receive ODM-207 due to rapidly deteriorating performance status). Based on the pharmacokinetic results from single-dose titration in the first cohort of patients no adjustment was required for the starting dose. One patient receiving a dose of 100 mg (1.9 mg/kg) experienced a DLT of intolerable grade 3 fatigue which was attributable to ODM-207 and this cohort (1A-2) was expanded. Although dose escalation continued up to the dose of 2 mg/kg without further dose-limiting toxicities, the safety monitoring board determined this to be the maximum tolerated dose due to ongoing intolerable fatigue and nausea, necessitating dose reduction and/or treatment discontinuation beyond the DLT period at this dose level. At the dose level of 2 mg/kg, five patients out of six had AEs resulting in dosing interruption and of these two patients required dose reductions and one discontinued the study. The dose-limiting toxicities and grade 3 adverse events are shown in Table 2.

Table 2 DLTs and grade 3 adverse events.

Safety

Overall 31 (89%) patients experienced a treatment-related AE. Treatment emergent related AEs, which occurred in a frequency of >10% of patients and/or of grade 3 severity are shown in Table 3. There were five deaths on study, all of which were considered not related to ODM-207 (one patient died of bowel obstruction, two of respiratory distress and two of disease progression). Common AEs included nausea (66%), thrombocytopaenia (51% of patients reported any grade), anorexia (49%), fatigue (43%), diarrhoea (40%), vomiting (40%), headache (37%) and weight loss (23%). Most common treatment-related grade ≥3 adverse events included thrombocytopaenia (14.3%), nausea (8.6%) and fatigue (5.7%).

Table 3 Related AEs.

Pharmacokinetics

Analysis of plasma pharmacokinetics showed slow absorption and elimination of ODM-207 with a median time to peak concentration between 2 and 6 h. Mean ODM-207 AUC(0-t) increased between day 1 and day 15 of dosing by 3.4-fold (n = 28, 95% CI 2.9, 3.8). Cohort mean total plasma concentrations on day 1 and day 15 (steady state) are presented in Fig. 1a. Plasma exposure to ODM-207 (AUC0-24) under fed condition was higher 1.5-fold than under fasting condition (Fig. 1b). The main metabolite is a demethylation product of ODM-207. The metabolite/parent ratio based in AUC(0-t) was 0.1–0.2 on day 1 and 0.1–0.5 on day 15.

Fig. 1: Pharmacokinetics of ODM-207.
figure 1

Cohort mean concentrations in fed-state on day 1 and day 15 (a), and in fed/fasted state in one cohort (b). a Cohorts 1A-1 0.6 mg/kg (blue square), Cohort 1A-2 1.3 mg/kg (green circle), Cohort 1A-3 1.5 mg/kg (orange diamond), and Cohort 1A-4 2.0 mg/kg (pink triangle). b Fasted state (blue square) and fed state (green circle).

Pharmacodynamics

As was shown with other BET inhibitors,13,14,23 a strong correlation was observed between ODM-207 plasma exposure and changes in platelet count over time. Prompt recovery of platelet count upon treatment interruption was observed (Fig. 2).

Fig. 2: Platelet count change over time.
figure 2

Mean platelet change from baseline (%) by pre-dose ODM-207 exposure (a) and examples of recovery of platelets upon dosing interruption (b). a Pre-dose concentration (ng/mL) <3500 (blue triangle), 3500–5000 (orange circle), and ≥5000 (green diamond). b Dosing interruption (orange circle) and resumption (green triangle).

Anti-tumour activity

Of the 27 patients with RECIST tumour response data, 21 were evaluable (completing ≥ 8 weeks of treatment). None had a partial or complete response (PR, CR). Six patients (29%) had stable disease (SD), 9 (43%) had progressive disease (PD), and 6 (29%) had non-CR/non-PD (non-measurable disease) as their best response. Eight patients (38%) had either SD or non-CR/non-PD at 16 weeks. The median (range) duration of SD was 16.3 (8.0–23.0) weeks. One out of four patients with NMC had SD as their best response, the other patients had clinical progression prior to their first disease assessment. The median progression-free survival was 2.2 months (range 0.5–9.7 months). Duration on treatment, ongoing RECIST response and reason for study discontinuation by patient’s tumour type are summarised in Fig. 3. There was no correlation between progression-free survival and increased exposure. None of the prostate cancer patients showed decline in PSA below baseline level.

Fig. 3: Duration of treatment and reason for discontinuation by cancer type.
figure 3

CRPC castration-resistant prostate cancer, ER + mBC oestrogen receptor positive metastatic breast cancer, NMC NUT midline carcinoma, NSCLC non-small cell lung cancer, RSCDS round small cells desmoplastic sarcoma, CRC colorectal carcinoma, SCLC small cell lung cancer, NHL non-Hodgkin’s lymphoma, SD stable disease, PD progressive disease, CR complete response, EOS end of study.

Discussion

The aim of this first-in-human Phase 1 study was to assess the safety and tolerability of the BET inhibitor ODM-207. The main dose-limiting adverse events were nausea and fatigue and the maximal tolerated dose of 2 mg/kg was identified. Other significant observed toxicities included asthenia and anorexia. Consistent with other studies of BET inhibitors, reversible thrombocytopaenia was observed,12,24 which further validates platelet count as a potential pharmacodynamic marker of on-target BET inhibition. We could not validate ODM-207’s therapeutic efficacy in NMC because the enrolled patients either progressed within days of enrolment (3 out of 4) or had very low ODM-207 plasma exposure (1 patient). None of these patients developed thrombocytopaenia. Thus, the lack of activity in these patients reflects inadequate exposure, which when coupled with an aggressive underlying disease, resulted in rapid disease progression.

A unique aspect of this clinical trial was the novel dose titration methodology utilised in the first cohort of patients. Plasma pharmacokinetic parameters were sampled after single dosing and were evaluated against preclinical models of exposure, providing confidence regarding the exposure and pharmacokinetic profiles in humans and mitigating the risk of inaccurate translations from models to humans and the risk for too low or too high dose in the first cohort of the first-in-human study. This methodology is particularly useful for first-in-human studies in which preclinical confidence in bioavailability is limited due to significant variability between models, or as in this case, where a narrow therapeutic window is expected.

In this study, modelling was performed over a short duration of 7 days, and also confirmed that exposure in the first cohort was at target level based on non-clinical data, demonstrating that rapid pharmacokinetic assessment is possible within a clinical trial setting whilst minimising the risk of inadequate exposure to this cohort of patients with advanced cancer. Although not demonstrated within this trial, this design could theoretically also reduce the number of dose escalation steps if dose titration was required, saving unnecessary burden from patients, either treated with a too low or high dose. Such a procedure can also significantly decrease development time and use of resources. In addition, the food-effect cohort included within this study, robustly demonstrated the association of the fed state with increased exposure. Overall at the higher dose levels, exposure was within the range expected for clinical activity. Target inhibition was confirmed with the consistent exposure-dependent decrease in platelet count observed across the study.

Multiple first-in-human studies of BET inhibitors have now been published, with the majority of compounds being analogues of JQ1, the first identified BET inhibitor.3 The chemical composition of ODM-207 is distinct from JQ1 analogues, but notably had overlapping profile of adverse events with other compounds.12,24 Consistently with other BET inhibitors, treatment-related adverse events limited dose escalation with most commonly observed AEs being thrombocytopenia, nausea, fatigue, decreased appetite, and diarrhoea.

Eight (38% of 21 evaluable patients) had stable disease or non-CR/non-PD for 16 weeks or more. The lack of significant responses is consistent with other studies of BET inhibitors, none of which have observed significant responses in solid tumours.11,12,24 The results of this study, when contextualised with the results of other Phase 1 studies of BET inhibitors in solid tumours, convey evidence of no strong signal of efficacy as a monotherapy in tumour types other than NMC. Mechanistically, the BET family of proteins control a diverse array of cellular processes6 and more preclinical work needs to be done prior to elucidation of the appropriate clinical context wherein BET inhibitors may be best utilised.25 The results of this trial herein, and emerging data from other Phase 1 studies of BET inhibitors in solid tumours, suggest that achieving a therapeutic window where an anti-tumour effect can be achieved without undue adverse events due to on-target effects may be challenging with this class of agents, also making drug combinations particularly difficult. Nevertheless, the diverse transcriptional networks impacted by BET inhibition have raised multiple combination opportunities, the goal being more favourable risk/benefit ratios. Of particular interest are combinations with immune checkpoint inhibitors,26,27 PARP inhibitors,28,29 CDK4/6 inhibitors or similar.30,31

In conclusion, evaluation of the BET inhibitor ODM-207 in this first-in-human study demonstrated dose proportional exposure with reversible thrombocytopaenia and in higher dose levels, fatigue and nausea, consistent with other studies of BET inhibitors. Strong signal for anti-tumour activity was not observed in patients with the selected advanced solid tumours.

References

  1. Marushige, K. Activation of chromatin by acetylation of histone side chains. Proc. Natl Acad. Sci. USA 73, 3937–3941 (1976).

    CAS  Article  Google Scholar 

  2. Zeng, L. & Zhou, M.-M. Bromodomain: an acetyl-lysine binding domain. FEBS Lett. 513, 124–128 (2002).

    CAS  Article  Google Scholar 

  3. Filippakopoulos, P., Qi, J., Picaud, S., Shen, Y., Smith, W. B., Fedorov, O. et al. Selective inhibition of BET bromodomains. Nature 468, 1067–1073 (2010).

    CAS  Article  Google Scholar 

  4. Yang, Z., Yik, J. H., Chen, R., He, N., Jang, M. K., Ozato, K. et al. Recruitment of P-TEFb for stimulation of transcriptional elongation by the bromodomain protein Brd4. Mol. Cell 19, 535–545 (2005).

    CAS  Article  Google Scholar 

  5. Jang, M. K., Mochizuki, K., Zhou, M., Jeong, H. S., Brady, J. N. & Ozato, K. The bromodomain protein Brd4 is a positive regulatory component of P-TEFb and stimulates RNA polymerase II-dependent transcription. Mol. Cell 19, 523–34. (2005).

    CAS  Article  Google Scholar 

  6. Shi, J. & Vakoc, C. R. The mechanisms behind the therapeutic activity of BET bromodomain inhibition. Mol. cell 54, 728–36. (2014).

    CAS  Article  Google Scholar 

  7. French, C. A. NUT midline carcinoma. Cancer Genet. Cytogenet. 203, 16–20 (2010).

    Article  Google Scholar 

  8. Chau N. G., Ma C., Danga K., Al-Sayegh H., Nardi V., Barrette R., et al. An anatomical site and genetic based prognostic model for patients with NUT midline carcinoma: analysis of 124 patients. JNCI Cancer Spectr. 4, pkz094 (2019).

    Article  Google Scholar 

  9. Stathis, A., Zucca, E., Bekradda, M., Gomez-Roca, C., Delord, J.-P., Rouge, Td. L. M. et al. Clinical response of carcinomas harboring the BRD4–NUT oncoprotein to the targeted bromodomain inhibitor OTX015/MK-8628. Cancer Discov. 6, 492–500 (2016).

    CAS  Article  Google Scholar 

  10. Zhang, Z., Ma, P., Jing, Y., Yan, Y., Cai, M.-C., Zhang, M. et al. BET bromodomain inhibition as a therapeutic strategy in ovarian cancer by downregulating FoxM1. Theranostics 6, 219 (2016).

    CAS  Article  Google Scholar 

  11. Postel-Vinay, S., Herbschleb, K., Massard, C., Woodcock, V., Ocker, M., Wilkinson, G. et al. First-in-human phase I dose escalation study of the Bromodomain and Extra-Terminal motif (BET) inhibitor BAY 1238097 in subjects with advanced malignancies. Eur. J. Cancer 69, S7–S8 (2016).

    Article  Google Scholar 

  12. Lewin, J., Soria, J.-C., Stathis, A., Delord, J.-P., Peters, S., Awada, A. et al. Phase Ib trial with birabresib, a small-molecule inhibitor of bromodomain and extraterminal proteins, in patients with selected advanced solid tumors. J. Clin. Oncol. 36, 3007–3014 (2018).

    CAS  Article  Google Scholar 

  13. Abramson J. S., Blum K. A., Flinn I. W., Gutierrez M., Goy A., Maris M., et al. BET inhibitor CPI-0610 is well tolerated and induces responses in diffuse large B-cell lymphoma and follicular lymphoma: preliminary analysis of an ongoing phase 1 study. Blood 126, 1491 (2015).

    Article  Google Scholar 

  14. Amorim, S., Stathis, A., Gleeson, M., Iyengar, S., Magarotto, V., Leleu, X. et al. Bromodomain inhibitor OTX015 in patients with lymphoma or multiple myeloma: a dose-escalation, open-label, pharmacokinetic, phase 1 study. Lancet Haematol. 3, e196–e204 (2016).

    Article  Google Scholar 

  15. Björkman M., Mattila E., Riikonen R., Abbineni C., Jaleel M., Marappan S., et al. ODM-207, a novel BET-bromodomain inhibitor as a therapeutic approach for the treatment of prostate and breast cancer. Cancer Res. 76, 4649 (2016).

    Google Scholar 

  16. Lindqvist J., Björkman M., Riikonen R., Nicorici D., Mattila E., Abbineni C., et al. Therapeutic targeting of estrogen receptor positive breast cancer with the BET bromodomain inhibitor ODM-207. Annals of Oncology. 29, viii1–viii13 (2018)

    Google Scholar 

  17. Moilanen A.-M., Björkman M., Riikonen R., Abbineni C., Jaleel M., Marappan S., et al. Targeting cancer with a novel BET bromodomain inhibitor ODM-207. Cancer Res. 77, 5074 (2017).

    Google Scholar 

  18. Lindqvist J., Björkman M., Riikonen R., Nicorici D., Mattila E., Jaleel M., et al. Antitumor activity of ODM-207, a novel BET bromodomain inhibitor, in nonclinical models of ER+ breast cancer as single agent and as a combination treatment. Cancer Res. 79, 3827 (2019).

    Google Scholar 

  19. Health UDo, Services H. Common terminology criteria for adverse events (CTCAE), v4. 03 2010. (National Cancer Institute, 2017)

  20. Eisenhauer, E. A., Therasse, P., Bogaerts, J., Schwartz, L. H., Sargent, D., Ford, R. et al. New response evaluation criteria in solid tumours: revised RECIST guideline (version 1.1). Eur. J. cancer 45, 228–247 (2009).

    CAS  Article  Google Scholar 

  21. Scher, H. I., Morris, M. J., Stadler, W. M., Higano, C., Basch, E., Fizazi, K. et al. Trial design and objectives for castration-resistant prostate cancer: updated recommendations from the Prostate Cancer Clinical Trials Working Group 3. J. Clin. Oncol. 34, 1402 (2016).

    Article  Google Scholar 

  22. Cheson, B. D., Fisher, R. I., Barrington, S. F., Cavalli, F., Schwartz, L. H., Zucca, E. et al. Recommendations for initial evaluation, staging, and response assessment of Hodgkin and non-Hodgkin lymphoma: the Lugano classification. J. Clin. Oncol. 32, 3059 (2014).

    Article  Google Scholar 

  23. O’Dwyer P. J., Piha-Paul S. A., French C., Harward S., Ferron-Brady G., Wu Y., et al. Abstract CT014: GSK525762, a selective bromodomain (BRD) and extra terminal protein (BET) inhibitor: results from part 1 of a phase I/II open-label single-agent study in patients with NUT midline carcinoma (NMC) and other cancers. Cancer Res. 76, CT014 (2016).

    Google Scholar 

  24. Piha-Paul S. A., Hann C. L., French C. A., Cousin S., Braña I., Cassier P. A., et al. Phase 1 study of molibresib (GSK525762), a bromodomain and extra-terminal domain protein inhibitor, in NUT carcinoma and other solid tumors. JNCI Cancer Spectr. 4, pkz093 (2019)

    Article  Google Scholar 

  25. Andrieu, G., Belkina, A. C. & Denis, G. V. Clinical trials for BET inhibitors run ahead of the science. Drug Discov. Today. Technol. 19, 45–50 (2016).

    Article  Google Scholar 

  26. Zhu, H., Bengsch, F., Svoronos, N., Rutkowski, M. R., Bitler, B. G., Allegrezza, M. J. et al. BET bromodomain inhibition promotes anti-tumor immunity by suppressing PD-L1 expression. Cell Rep. 16, 2829–2837 (2016).

    CAS  Article  Google Scholar 

  27. Jing X., Shao S., Zhang Y., Luo A., Zhao L., Zhang L., et al. BRD4 inhibition suppresses PD-L1 expression in triple-negative breast cancer. Exp. Cell Res. https://doi.org/10.1016/j.yexcr.2020.112034 (2020)

  28. Yang L., Zhang Y., Shan W., Hu Z., Yuan J., Pi J., et al. Repression of BET activity sensitizes homologous recombination-proficient cancers to PARP inhibition. Sci. Transl. Med. 9, eaal1645 (2017)

  29. Karakashev, S., Zhu, H., Yokoyama, Y., Zhao, B., Fatkhutdinov, N., Kossenkov, A. V. et al. BET bromodomain inhibition synergizes with PARP inhibitor in epithelial ovarian cancer. Cell Rep. 21, 3398–3405 (2017).

    CAS  Article  Google Scholar 

  30. Liao, S., Maertens, O., Cichowski, K. & Elledge, S. J. Genetic modifiers of the BRD4-NUT dependency of NUT midline carcinoma uncovers a synergism between BETis and CDK4/6is. Genes Dev. 32, 1188–200. (2018).

    CAS  Article  Google Scholar 

  31. Shu S., Wu H. J., Ge J. Y., Zeid R., Harris I. S., Jovanovic B., et al. Synthetic lethal and resistance interactions with BET bromodomain inhibitors in triple-negative breast cancer. Mol Cell. 78, 1096–1113.e8 (2020)

Download references

Acknowledgements

We thank the patients who participated in this trial and their families. We are also grateful to all investigators and the study teams at the participating centres.

Author information

Authors and Affiliations

Authors

Contributions

Authors (J.DB., I.B., P.B., S.P-V., J.A.,T.K., A.S.) contributed to and were involved in the conception and design of the study, provision of study materials or patients (M.A., I.B., P.B., S.P-V., R.P., J.DB.), collection and assembly of data, data analysis and interpretation, and paper writing (M.A., I.B., P.B., S.P-V., R.P., J.A.,T.K., A.S., J.DB.). All authors (M.A., I.B., P.B., S.P-V., R.P., J.A., T.K., A.S., J.DB.) read and approved the final paper.

Corresponding author

Correspondence to Johann S de Bono.

Ethics declarations

Ethics approval and consent to participate

The protocol was approved by the institutional review boards and independent ethics committees of the participating centres and registered under the NCT03035591 identifier at the clinicaltrials.gov website. The study was performed in accordance with the Declaration of Helsinki and was conducted in compliance with the International Conference on Harmonization on Good Clinical Practice and written informed consent was required from all patients prior to enrolment.

Data availability

The datasets generated and/or analysed during the current study are not publicly available due proprietary restrictions but are available from the corresponding author on reasonable request.

Competing interests

All authors have completed the Unified Competing Interest form (http://www.icmje.org/coi_disclosure.pdf) and declare: I.B. reports scientific consultancy role for Orion Pharma. P.B. reports personal fees from Orion Pharma, during the conduct of the study; personal fees from BMS, MSD, Pfizer, Novartis, Oncorena, TILT Biotherapeutics, Faron Pharmaceuticals, Ipsen, Herantis Pharma, outside the submitted work; and stock ownership: TILT Biotherapeutics and Terveystalo. S.P-V. has received research funding from Merck KGaA, Boehringer Ingelheim and Institut Roche for unrelated research projects. SPV has participated to advisory boards for Merck KGaA. As part of the Drug Development Department (DITEP), SPV is principal investigator or sub-investigator of clinical trials from Abbvie, Agios Pharmaceuticals, Amgen, Argen-X Bvba, Arno Therapeutics, Astex Pharmaceuticals, Astra Zeneca, Aveo, Bayer Healthcare Ag, Bbb Technologies Bv, Blueprint Medicines, Boehringer Ingelheim, Bristol Myers Squibb, Celgene Corporation, Chugai Pharmaceutical Co., Clovis Oncology, Daiichi Sankyo, Debiopharm S.A., Eisai, Eli Lilly, Exelixis, Forma, Gamamabs, Genentech, Inc., Glaxosmithkline, H3 Biomedicine, Inc, Hoffmann La Roche Ag, Innate Pharma, Iris Servier, Janssen Cilag, Kyowa Kirin Pharm. Dev., Inc., Loxo Oncology, Lytix Biopharma As, Medimmune, Menarini Ricerche, Merck Sharp & Dohme Chibret, Merrimack Pharmaceuticals, Merus, Millennium Pharmaceuticals, Nanobiotix, Nektar Therapeutics, Novartis Pharma, Octimet Oncology Nv, Oncoethix, Onyx Therapeutics, Orion Pharma, Oryzon Genomics, Pfizer, Pharma Mar, Pierre Fabre, Roche, Sanofi Aventis, Taiho Pharma, Tesaro Inc, and Xencor. R.P. reports other from Celgene, during the conduct of the study. J.A., T.K. and A.S. are employees of Orion Corporation Orion Pharma. J.DB. has served on advisory boards and received fees from many companies including Astra Zeneca, Astellas, Bayer, Boehringer Ingelheim, Cellcentric, Daiichi, Genentech/Roche, Genmab, GSK, Janssen, Merck Serono, Merck Sharp & Dohme, Menarini/Silicon Biosystems, Orion, Pfizer, Qiagen, Sanofi Aventis, Sierra Oncology, Taiho, Vertex Pharmaceuticals. He is an employee of The ICR, which have received funding or other support for his research work from AZ, Astellas, Bayer, Cellcentric, Daiichi, Genentech, Genmab, GSK, Janssen, Merck Serono, MSD, Menarini/Silicon Biosystems, Orion, Sanofi Aventis, Sierra Oncology, Taiho, Pfizer, Vertex, and which has a commercial interest in abiraterone, PARP inhibition in DNA repair defective cancers and PI3K/AKT pathway inhibitors (no personal income). He was named as an inventor, with no financial interest, for patent 8,822,438. He has been the CI/PI of many industry sponsored clinical trials. JDB is a National Institute for Health Research (NIHR) Senior Investigator. The views expressed in this article are those of the author(s) and not necessarily those of the NHS, the NIHR, or the Department of Health. The remaining authors declare no competing interests.

Funding information

The study was sponsored by Orion Corporation, Orion Pharma, Espoo, Finland, the developer of ODM-207.

Additional information

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary information

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Ameratunga, M., Braña, I., Bono, P. et al. First-in-human Phase 1 open label study of the BET inhibitor ODM-207 in patients with selected solid tumours. Br J Cancer 123, 1730–1736 (2020). https://doi.org/10.1038/s41416-020-01077-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/s41416-020-01077-z

Further reading

Search

Quick links